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Method for extracting steroidal compounds from Scapharca subcrenata

A technology of steroidal compounds and extracts, applied in the field of marine natural product chemistry, can solve the problems of less research on anti-insect activity, and achieve the effect of simple preparation method, low pollution and simple operation

Inactive Publication Date: 2015-03-04
广西海洋研究所有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It is well known that steroids are a kind of common natural chemical components in nature, and are important raw materials for the production of hormone drugs, but there are few studies on their anti-insect activity. A basis has been established, which will broaden the medicinal field of cockles, which is the significance of this application

Method used

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  • Method for extracting steroidal compounds from Scapharca subcrenata
  • Method for extracting steroidal compounds from Scapharca subcrenata
  • Method for extracting steroidal compounds from Scapharca subcrenata

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Embodiment 1

[0020] The preparation method of clam extract:

[0021] Take 15kg of fresh cockles, wash, remove the shell, and take the meat, homogenate in a tissue masher, extract 4 times with 95% ethanol, each time for 1 day, concentrate the extract to obtain 71.2g of extract, and mix the extract with 500ml of water Fully suspend, then extract with the same volume of petroleum ether, ethyl acetate, and n-butanol, and concentrate under reduced pressure at 100–300hPa to obtain 38.5g of petroleum ether phase, 13.5g of ethyl acetate phase, and 17.3g of n-butanol phase and aqueous phase 414.0g, using silica gel column chromatography, gel column chromatography, high performance liquid and other means to separate and purify the n-butanol phase to obtain 3 pure compounds, respectively 8.5mg compound 1, 11.9mg compound 2 and 5.2 mg of compound 3, the physical and chemical properties and spectral data of the compound were analyzed, and compared with the literature, the three compounds were identifie...

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Abstract

The invention discloses a method for extracting steroidal compounds from Scapharca subcrenata. Currently, researches on Scapharca subcrenata are mostly focused on active polysaccharides, polypeptides, proteins and the like, and no technique for extracting the steroidal compounds from Scapharca subcrenata is disclosed yet at present. According to the method, by carrying out the technical steps of: (1) extracting alcohol-soluble extractives from the Scapharca subcrenata, (2) preparing Scapharca subcrenata extractants, (3) extracting the steroidal compounds, (4) analyzing physicochemical properties and spectrum data of the three pure compounds, and the like, normal butanol phases in extraction liquid in the alcohol-soluble extractives are separated and purified to obtain cholesterol, cholestanol and progesterone; after being tested, all the three steroidal compounds have good activities in killing of artemia, the fatality rates to artemia respectively are 65.0%, 58.3% and 60% when the concentrations are 50 micrograms per microliter, and the artemia killing rates rise with the increase of concentrations, so that the concentration dependency is expressed; and the development period of a new medicine can be greatly shortened by utilizing the method, and the method is significant to research and development of the new medicine.

Description

technical field [0001] The invention relates to a method for extracting steroidal compounds from cockles, belonging to the field of marine natural product chemistry. Background technique [0002] Hair clam (Scapharca subcrenata), also known as "hair clam, hemp clam", is a mollusc Phylumbranchia Scapharca clam family. It is distributed in the coastal areas of China, Korea and Japan, especially in the Bohai Sea and the East China Sea. Clams have high nutritional and medicinal value. They have the effects of resolving phlegm, softening firmness, dispelling blood stasis, and eliminating stagnation. They can treat diseases such as phlegm accumulation, stomach pain, noise, acid regurgitation, scarring, scrofula, and dental malnutrition. It is now widely used In the clinical treatment of gastric and duodenal ulcers, there are medicinal records in "Shen Nong's Materia Medica" and the main herbal medicines of the past dynasties. It is used in folks for anti-tumor, anti-anemia and ant...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J9/00C07J7/00A01P7/00
Inventor 徐艳孙雪萍童万平王志成
Owner 广西海洋研究所有限责任公司
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