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Preparation method and application of low-temperature efficient corn straw degrading bacterial agent

The technology of corn stalk and degrading bacteria is applied in the field of preparation of low-temperature and high-efficiency corn stalk degrading bacteria agent, which can solve the problems of inappropriate use and the like, and achieve the effects of low cost and simple method.

Active Publication Date: 2021-05-18
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the growth process of microorganisms is greatly affected by the environment. Most of the straw-degrading microorganisms found through screening at present only have the ability to partially degrade straw at room temperature or high temperature, and are not suitable for use in low-temperature environments in northern winter.

Method used

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  • Preparation method and application of low-temperature efficient corn straw degrading bacterial agent
  • Preparation method and application of low-temperature efficient corn straw degrading bacterial agent
  • Preparation method and application of low-temperature efficient corn straw degrading bacterial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: Fermentation of bacterial liquid of the present invention

[0043] (1) Sources and types of bacteria:

[0044] The two bacterial strains were isolated from the soil of the Science and Technology Park of the Vocational and Technical College of Inner Mongolia Agricultural University;

[0045] Two single bacterial strains are: Pseudomonas and Acinetobacter,

[0046] The Pseudomonas is preserved in the General Biological Center of the China Microorganism Culture Collection Management Committee. The address of the preservation unit is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing. The preservation date is: August 11, 2020, and the classification is named. : Pseudomonas sp., the deposit number is CGMCC No.20521;

[0047] The Acinetobacter is preserved in the General Biological Center of the China Microbial Culture Collection Management Committee. The address of the preservation unit is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing. The...

Embodiment 2

[0061] Embodiment 2: bacterial agent preparation

[0062] (1) Test material

[0063] Bacterial classification: with embodiment 1

[0064] Carrier: corn stalk powder (through 60 mesh sieve)

[0065] Protective agent: starch

[0066] (2) Preparation method of bacterial agent

[0067] Mix 7.5g of the carrier (corn husk straw powder) and protective agent (starch) in an aluminum box culture dish with a diameter of 12cm and a height of 2cm. After closing the lid, fix it with a rubber band and place it in a high-temperature sterilization pot (temperature : 121°C, time: 20min) after sterilization for later use.

[0068] After the fermentation of the bacterial liquid, the number of viable bacteria reached 1.45×10 10 . Dispense the fermented bacterial liquid into the above-mentioned aluminum box petri dish with sterilized fillers under aseptic conditions, so that the ratio of the bacterial liquid to the filler (the carrier and the protective agent are collectively referred to as t...

Embodiment 3

[0074] Embodiment 3: Study on field application effect of bacterial agent

[0075] (1) Preparation of corn stalks:

[0076] Harvested corn stalks. Cut the straw into pieces with a length of about 5cm, and ensure the integrity of the inner pulp and outer skin of the straw, and then pack each 30g of straw into red gauze bags with a pore size of 5mm, and prepare to be buried in the ground.

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Abstract

The invention relates to the field of microorganisms, and particularly discloses a preparation method and application of a low-temperature efficient corn straw degrading bacterial agent. The bacterial agent is prepared from two single-plant bacteria, wherein the two single-plant bacteria are Pseudomonas sp. and Acinetobacter sp. and the preservation numbers are CGMCC No.20521 and CGMCC No.20522 respectively. According to the preparation method, the specific-composition bacteria genus is used as a bacteria source sample, a culture medium adapted to the bacteria source sample is used to perform fermentation propagation, the bacterial liquid fermented under the optimal fermentation condition cooperates with a suitable carrier and a protective agent, and a freeze drying technology is adopted to prepare the powdery bacterial agent. The bacterial agent can degrade corn straw under the low temperature condition, the preparation method is simple, the cost is low, the production period is short, and the problems that agricultural production is inconvenient due to the fact that straw cannot be degraded under the low-temperature condition after straw returning to the field in the north can be solved.

Description

technical field [0001] The invention relates to the field of configuration of microbial inoculants, in particular to a preparation method and application of a low-temperature and high-efficiency corn stalk degrading inoculant. Background technique [0002] Straw returning is one of the effective measures to improve soil fertility. It is to mechanically pulverize field crop straws and then return them to the field in situ. It has the advantages of reducing the amount of chemical fertilizers, increasing organic matter, improving soil structure, and reducing environmental pollution. However, in the cold and arid regions of northern my country, the temperature drops quickly after harvest and enters winter, and the low temperature in winter makes the returning straw not fully degraded, which affects a series of problems such as the sowing quality, seedling emergence rate and agricultural operation in the next spring, which seriously affects the popularization of this technology. ....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04C05F17/20C05F17/50C05G3/80C12R1/38C12R1/01
CPCC12N1/20C12N1/04C05F11/00C05F11/08C05F17/20C05F17/50C05G3/80Y02W30/40
Inventor 高聚林张赛楠于晓芳韩升才青格尔胡树平马达灵屈佳伟王志刚孙继颖
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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