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A kind of sea wormwood polysaccharide with anti-oxidation and regulating intestinal flora effect and its preparation method and application

A technology for regulating intestinal flora and Artemisia japonica polysaccharide, applied in the field of polysaccharides, can solve the problems of high energy consumption, high reaction temperature, long reaction time, etc., and achieves high degradation efficiency, mild reaction conditions, and simple and easy-to-operate equipment. Effect

Active Publication Date: 2022-04-22
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method can prepare polysaccharides with both antioxidant and hypoglycemic activities, but this method has the disadvantages of long reaction time, high reaction temperature, and high energy consumption.

Method used

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  • A kind of sea wormwood polysaccharide with anti-oxidation and regulating intestinal flora effect and its preparation method and application
  • A kind of sea wormwood polysaccharide with anti-oxidation and regulating intestinal flora effect and its preparation method and application
  • A kind of sea wormwood polysaccharide with anti-oxidation and regulating intestinal flora effect and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038]1) After drying the Artemisia annua, crush it, pass through a 60-mesh sieve, mix the Artemisia Artemisiae powder with 95% ethanol at a mass volume ratio of 1g:3mL, add 95% ethanol, heat and reflux for 3 hours at a temperature of 70°C, and centrifuge After 20 minutes, the residue was separated, and the residue was repeated twice, and dried at 45°C for 24 hours;

[0039] 2) Mix the dry powder pretreated in step 1) with water at a mass-to-liquid ratio of 1 g: 20 mL, and extract at a temperature of 80° C. for 2 hours and 2 extraction times; centrifugal separation, Combine the extracts, concentrate under reduced pressure at 50°C to 1 / 4 of the original volume, and obtain the concentrated solution of Artemisia annua polysaccharide;

[0040] 3) Add Sevag reagent to the concentrated solution of Artemisia polysaccharides, the volume ratio of the concentrated solution of Artemisia polysaccharides to Sevag reagent (chloroform:n-butanol=3:1) is 4:1, shake for 30min, and centrifuge to...

Embodiment 2

[0045] 1) After drying the sea wormwood, crush it, pass through a 60-mesh sieve, add 95% ethanol according to the mass volume ratio of the sea wormwood powder to 95% ethanol, heat and reflux for 4 hours at a temperature of 70°C, and centrifuge for 20 minutes , the residue was separated, and the residue was repeated twice for the above operation, and dried at 45°C for 24h;

[0046] 2) Mix the dry powder pretreated in step 1) with water at a mass-volume ratio of 1g:20mL, and extract at a temperature of 80°C for 2 hours and 3 extraction times; centrifugal separation, Combine the extracts, concentrate under reduced pressure at 50°C to 1 / 4 of the original volume, and obtain the concentrated solution of Artemisia annua polysaccharide;

[0047] 3) Add Sevag reagent to the concentrated solution of Artemisia polysaccharides, the volume ratio of the concentrated solution of Artemisia polysaccharides to Sevag reagent (chloroform:n-butanol=3:1) is 4:1, shake for 30min, and centrifuge to r...

Embodiment 3

[0052] 1) After drying the sea wormwood, crush it, pass through a 60-mesh sieve, add 95% ethanol according to the mass volume ratio of sea wormwood powder and 95% ethanol, heat and reflux for 3 hours at a temperature of 70°C, and centrifuge for 20 minutes , the residue was separated, and the residue was repeated twice for the above operation, and dried at 50°C for 24 hours;

[0053] 2) Mix the pretreated dry powder in step 1) with water at a mass-volume ratio of 1 g: 20 mL, and extract at a temperature of 80° C. for 2 hours and 4 extraction times; centrifugal separation, Combine the extracts, concentrate under reduced pressure at 50°C to 1 / 10 of the original volume, and obtain the concentrated solution of Artemisia annua polysaccharide;

[0054] 3) Add Sevag reagent to the concentrated solution of Artemisia polysaccharides, the volume ratio of the concentrated solution of Artemisia polysaccharides to Sevag reagent (chloroform:n-butanol=3:1) is 4:1, shake for 30min, and centrif...

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Abstract

The invention discloses a sea wormwood polysaccharide with the functions of anti-oxidation and regulating intestinal flora, as well as a preparation method and application thereof. The preparation method includes hot water extraction, deproteinization, decolorization, alcohol precipitation, dialysis and degradation; wherein, the degradation process is to add the crude polysaccharide solution of Artemisia annua with a concentration of 5-8 mg / mL, and add H 2 o 2 The solution reaches a volume concentration of 1-5%, is placed in an ultrasonic field for radiation treatment, the ultrasonic frequency is 20-25kHz, and the power is 450-550W, and freeze-dried to obtain sea wormwood polysaccharide. Compared with the crude polysaccharide of sea wormwood, the average molecular weight of the sea wormwood polysaccharide of the present invention is 130-510kDa, the particle size is 400-500nm, and the viscosity is low, which can significantly increase the abundance of beneficial bacteria in the human intestinal tract, while reducing harmful bacteria The abundance of intestinal microorganisms increases by about 7%, and it can be used as a food additive in functional foods that regulate intestinal health.

Description

technical field [0001] The invention relates to the field of polysaccharides, in particular to a sea wormwood polysaccharide with the functions of anti-oxidation and regulating intestinal flora, its preparation method and application. [0002] technical background [0003] Artemisia annua, also known as "big-leaf seaweed", belongs to the Phaeiophyta Sargassum family and is widely distributed in warm temperate waters. Haihaozi is widely distributed in the Bohai Sea, Yellow Sea, East China Sea and other regions of China. It is dark brown and is a typical marine brown algae. It has a bitter, salty and cold taste and enters the spleen, kidney and lung. Functions and indications Cure diuresis, expel heat, painful nuclei, chronic bronchitis, etc., and have high medicinal value. Sea Artemisia contains a large amount of saturated and unsaturated fatty acids, and the content of unsaturated fatty acids is much higher than that of saturated fatty acids. Sea Artemisia is rich in variou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A23L33/125A61P1/00A61P39/06
CPCC08B37/006C08B37/0003A61P39/06A61P1/00A23L33/125A23V2002/00A23V2200/02A23V2200/3202A23V2250/50
Inventor 袁旦李超扶雄
Owner SOUTH CHINA UNIV OF TECH
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