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Application of Callerya speciosa endophyte RH5 in promotion of growth of Callerya speciosa

A technology of cattle vigorous and endophyte, applied in application, plant growth regulator, plant growth regulator and other directions, can solve the problem of long growth cycle of cattle, and achieve the effect of improving yield and quality

Active Publication Date: 2020-09-18
GUANGXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the growth cycle of Niu Dali is long, and it usually takes 6-8 years to harvest. How to shorten the planting cycle of Niu Dali and improve the yield and quality of medicinal materials has become a major problem to be solved urgently

Method used

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  • Application of Callerya speciosa endophyte RH5 in promotion of growth of Callerya speciosa
  • Application of Callerya speciosa endophyte RH5 in promotion of growth of Callerya speciosa
  • Application of Callerya speciosa endophyte RH5 in promotion of growth of Callerya speciosa

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Experimental program
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Embodiment

[0024] 1. Isolation and purification of strains

[0025] Test material: Calleryaspeciosa (Champ.ex Benth) Schot collected from Guangxi Guangze Health Industry Co., Ltd.

[0026] NA medium: Each liter of NA medium contains 3 g of beef extract, 1 g of yeast extract, 5 g of peptone, 10 g of sucrose, and 15 g of agar, and its pH is 7.0.

[0027] Rinse the fresh and healthy burdock root with a length of 5-6cm with running water, and then move it to the ultra-clean workbench. Sterilize with 0.1% mercuric chloride solution for 8-10 minutes, wash with sterile water for 4-5 times after sterilization, and dry the surface moisture with sterile filter paper for later use.

[0028] Scrape off the sterilized beef root skin, cut into 1cm-long root segments, put them into a sterile mortar, add 5mL of sterile water for grinding, and transfer the grinding liquid to a sterile centrifuge tube after the grinding is complete. Set aside for 10 minutes, take 0.5mL supernatant for serial dilution of...

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Abstract

The invention discloses application of Callerya speciosa endophyte RH5 in promotion of the growth of Callerya speciosa. The classification name of Callerya speciosa endophyte RH5 is Burkhoideria sp. RH5, and is submitted to the Guangdong Province Microbial Collection on May 29th, 2020, and the preservation number is GDMCC NO: 61030. The endophyte RH5 is added during the growth of Callerya speciosa, and the strain is capable of promoting the growth of Callerya speciosa and improving the yield and quality of the Callerya speciosa medicinal material.

Description

technical field [0001] The invention relates to the field of biotechnology, and more specifically relates to the application of a bovine vigorous endophyte RH5 in promoting the growth of bovine vigorous. Background technique [0002] Plant endophytes refer to fungi or bacteria that live in the tissues and organs of healthy plants for a certain period or all periods. Ubiquitously present in higher plants, endophytic bacteria are found in woody, herbaceous, monocotyledonous and dicotyledonous plants. During the long-term co-evolution process, a stable mutualistic symbiotic relationship has been formed between endophytes and host plants. The host provides the nutrients needed for growth and a relatively stable living environment for endophytes. Endophytes live in plants and largely avoid various severe competitive pressures in the external environment; while endophytes The metabolites produced in its own life process can promote the growth and development of host plants and i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/20A01P21/00A01G22/40A01C21/00C05F11/08C12R1/01
CPCA01N63/20A01G22/40A01C21/005C05F11/08C12R2001/01C12N1/205
Inventor 黄荣韶黄英李良波谭勇姚绍嫦黄鼎
Owner GUANGXI UNIV OF CHINESE MEDICINE
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