212 results about "Millettia speciosa" patented technology

The invention discloses a radix millettiae speciosae extract and an application of the radix millettiae speciosae extract in preparing drugs for treating osteoporosis. The radix millettiae speciosae extract is obtained by drying the roots of radix millettiae speciosae and crushing into powder, then refluxing with ethanol and extracting, filtering an extracting solution, decompressing and recycling a solvent completely. The radix millettiae speciosae extract is discovered with a good efficacy in treating osteoporosis on a basis of efficacy test, so that the radix millettiae speciosae extract can be used for preparing drugs for treating osteoporosis, and an effective way is provided for the treatment of osteoporosis, and moreover, the preparation method is simple, and the cost is low.

The invention discloses a method for directly inducing cluster buds of milletia speciosa champ seeds and quickly breeding seedlings. According to the method, cluster buds of milletia speciosa champ seeds are directly induced by an explant; and the method for breeding seedlings comprises explant sterilization, primary induction, secondary multiplication, rooting and transplanting to obtain milletia speciosa champ seedlings. The method disclosed by the invention has the advantages of simplicity and convenience in operation, low production cost, fast multiplication and high survival rate of rooting and transplanting, facilitates large-scale industrial production and application, can quickly and continuously obtain a great quantity of high-quality milletia speciosa champ test-tube seedlings, and has high practicability.

The invention discloses a method for propagating Millettia Speciosa germchit by cuttage, including first-phase preparation and later management: 1) during the first-phase preparation, Millettia Speciosa tresses are selected and cut into stem segments for spare use; river sand and coco bran are evenly mixed according to certain proportion to obtain a substrate for spare use; 2) during the later management, Millettia Speciosa stem segments are dipped with root stimulate at the base parts and then stuck into the substrate; the stem segments are watered to promote the close contact between the base parts thereof and the substrate; the stuck stem segments are shaded for culture, with the moisture of the substrate and air humidity being guaranteed; after taking root, the stem segments are fertilized with urea solution until the germchit grows up. The invention has the advantages of simple process flow and low manufacturing cost, and, effectively utilizes the scrap Millettia Speciosa tresses; by optimizing the root stimulate and the substrate formula, the invention improves the culture conditions and increases the survival rate of the stuck stem segments. The method has high feasibility, simple operation, low cost input and high survival rate and achieves the dual purpose of recycled utilization of resources and batch production of the germchit.

The present invention relates to a plantation method of millettia speciosa champ, and belongs to the agriculture plantation field. The plantation method specifically comprises the following steps of selecting the land suitable for planting the millettia speciosa champ, applying base fertilizer prepared by mixing self-made farm manure, plant ashes and quicklime according to a certain ratio, and plowing and bedding, wherein the self-made farm manure is prepared by mixing millettia speciosa champ litters, corn stalks, wheat straws, coconut shells and soybean meal with pig, cattle and sheep manure and night soil; then selecting millettia speciosa champ seedlings, transplanting the millettia speciosa champ seedlings in the land, and covering a membrane; and fertilizing once a year, timely cutting tails and cutting flowers, paying attention to insect disease prevention, and harvesting the millettia speciosa champ after cultivating the millettia speciosa champ for three years. The plantation method is simple in operation and high in practicality, and is suitable for large scale systematic breeding, diseases and pests can be reduced and prevented, the plant survival rate is high, growth speed is fast, and production cost is low.

The invention provides a method for tissue culturing and reproducing the millettia speciosa champ germchit. By using the tissue culture method and taking the fresh apical bud and the axillary bud of the wild millettia speciosa champ as the explant, the explant is disinfected and sterilized by the 75 percent alcohol and 0.1 percent mercuric chloride solution; after that, according to different periods, different culture medium formulas are adopted respectively for the bud induction culture, bud subculture proliferation and the rooting culture to obtain the container seedling of the millettia speciosa champ, wherein, the bud induction culture medium has a formula of MS+6-BA0.4mg.L-1+IBA0.2mg.L-1+3 percent white granulated sugar+0.35 percent agar, the formula of the bud subculture proliferation culture medium is MS+6-BA0.6mg.L-1+NAA0.5mg.L-1+3 percent white granulated sugar+0.35 percent agar, and the formula of the rooting culture medium is 1 / 2MS+ABT10.8mg.L-1+IBA0.4mg.L-1+3 percent white granulated sugar+0.35 percent agar; moreover, when the height of the container seedling is grown about 2 cm, the container seedling is again transplanted to a seedbed of a greenhouse for culturing; after reaching over 20cm, the container seedling is the grown seedling of the millettia speciosa champ germchit.

The invention discloses a method for rapid propagation of beautiful millettia root germchits by using test tube micro-cuttage, which comprises: beautiful millettia root seeds are inoculated into the culture medium for germination and cultivated until the seeds grow into seedlings; the seedlings obtained are cut into stem sections, inserted into the micro-cuttage culture medium and cultivated until the lateral buds come out, then, the lateral buds are cut into stem sections and inserted into a fresh micro-cuttage culture medium for cultivation; after being cut into stem sections, the lateral buds obtained are inoculated into a rooting medium and cultivated until the stem sections take root, thereby obtaining the beautiful millettia root germchits. The technique is simple and easy, has the advantages of simplicity in operation and high propagation rate, solves the problems of small seeds number, low germinating capacity and low propagation rate of germchits propagation by using beautiful millettia root seeds, and realizes long-term large-scale rapid propagation of beautiful millettia root germchits. The technique can obtain high quality beautiful millettia root test tube sprouts in large quantity quickly and continuously, and has strong practicability.

The invention discloses a rapid propagation method for suspension cell culture of Millettia speciosa Champ. According to the rapid propagation method, young and tender cotyledons taken from Millettia speciosa Champ pods 50-60 days after flowering are utilized as culture materials, subcultured callus tissue is obtained through inducing culture and subculture, and a stable Millettia speciosa Champ suspension cell culture fluid is obtained through the suspension cell culture. With the adoption of the method, stable Millettia speciosa Champ suspension cells with good quality and high propagation yield can be obtained, and a novel way and method are provided for development and utilization of effective components of the Millettia speciosa Champ and creation of new germplasm resources from the Millettia speciosa Champ with biotechnology methods such as somatic hybridization, genetic transformation, mutant induction and the like.

The invention discloses a preparing method for health care liquor with an immunity boosting function. The preparing method includes the following steps of 1 raw material processing, wherein raw materials include, by weight, 20-30 parts of beautiful millettia roots, 10-15 parts of barrenwort and 3-5 parts of medlar, the beautiful millettia roots are cleaned, sliced and smashed after being dried in the sun, the medlar and the barrenwort are cleaned, aired in a shady, cool and ventilating place and then smashed; 2 high-pressure processing, wherein the beautiful millettia roots obtained in the step 1 are put into a high-pressure tank, the high-pressure tank is pressurized until the pressure is 20-30 MPa, the pressure is uniform and kept for 10-20 min, and then normal pressure is recovered; 3 steam explosion processing, wherein the medlar and the barrenwort obtained in the step 1 are put into a steam explosion tank, and the explosion pressure is 3.0-3.5 MPa and kept for 150-200 s; 4 soaking, wherein the raw materials obtained from the step 2 and the step 3 are mixed evenly and put into a container, rice wine is poured into the container, and the container is capped and sealed to soak the raw materials; 5 postprocessing, wherein a soak solution obtained from the step 4 is taken out, filtered and subpackaged. The invention further relates to health care liquor obtained by adopting the method, and the health care liquor is golden, clear and transparent, and has a delicious mouthfeel and a slight smell of traditional Chinese medicines.

The invention discloses a quality control method of a loins-strengthening and kidney-invigorating medicine. In the method, beautiful millettia root, rhizoma cibotii, kadsura coccinea, caulis spatholobi, parasitic loranthus and glossy privet fruit in the medicine are respectively identified by a thin layer chromatography; and the content of protocatechuic acid and the content of protocatechuic aldehyde in the medicine are measured via a liquid chromatography. In the quality control method established by the invention, the medicine material identifying method is mature and feasible, strong in specificity, negative and interference-free; the content measurement method is easy in operation, high in precision and good in reproducibility; and by adopting the quality control method disclosed by the invention, the quality of the medicine can be precisely and stably controlled to adapt to the industrial and stable production of the medicine.

The invention belongs to the field of medical healthy food, and relates to a millettia speciosa health tea bag. The preparation method for the healthy tea bag comprises the following steps: slicing a millettia speciosa material, baking to be cooked, baking till the slices are completely dried, and crushing the slices into powder for later use; breaking siraitia grosvenorii into fragments, baking till the fragments are completely dried, and crushing the fragments into powder for later use; uniformly mixing the millettia speciosa powder and the siraitia grosvenorii powder, bagging and sealing to obtain finished products. The healthy tea bag is simple in preparation method; millettia speciosa is adopted as the main raw material, siraitia grosvenorii is adopted as the additional raw material, and the characteristics that millettia speciosa and siraitia grosvenorii have the same medicine properties and the similar functions are utilized for scientific compatibility to balance the medicine properties and to supplement each other, so that the effects of the ingredients can be given full play, the comfortable taste is maintained, the effects of clearing heat, moistening lungs, relaxing tendons, activating collaterals and the like are enhanced, people's heath is invigorated, resistance to diseases is improved, and the health tea bag is suitable for people to drink for a long term.

The invention discloses a traditional Chinese medicine feed additive for livestock as well as a preparation method and an application of feed additive. According to the formula, the traditional Chinese medicine feed additive comprises following components in parts by weight: 100 parts of bagasse polysaccharide, 5-20 parts of houttuynia cordata, 5-20 parts of pericarpium citri reticulatae, 2-10 parts of millettia specisoa and 2-10 parts of areca-nuts. Compared with the prior art, the feed additive has the advantages that all raw materials are natural plants and do not contain any chemical additive; the traditional Chinese medicine feed additive for the livestock, which is prepared by taking the bagasse polysaccharide with a good mouth feel as the main raw material and matching the houttuynia cordata, the pericarpium citri reticulatae, the millettia specisoa, the areca-nuts and the like, has the effects of enhancing the animal immunologic function, improving the resistance and the stress resistance of the livestock to infectious diseases, promoting the animal growth and improving the feed conversion rate; the problem of agricultural wastes can also be solved and wastes are changed into valuable things.

The invention discloses a traditional Chinese medicine foot bath powder and a preparation method thereof. The foot bath powder comprises the following raw materials: lobelia, cnidium fruit, spatholobus stems, matrine,dictamni cortex, septemlobate kalopanax branchlet, buddleia officinalis maxim, beautiful millettia root, bladhia punctata nakai, campsis grandiflora, tuber fleeceflower stems, clematis root, refined sea salt and boric acid. The pharmacological effect of the traditional Chinese medicine foot bath powder is that hot water promotes the medicine to permeate into a human body, so that the medicine thoroughly going to the whole body main and collateral channels of the body through feet can be ensured, and adverse reaction caused by overmuch oral medicine can be prevented; efficacies of dredging the channel, improving the blood circulation, promoting the metabolization, adjusting a nervous system, improving the sleep quality, and preventing and curing all kinds of vascular diseases are realized after the medicine is used for a long time.

The invention provides cassava rice which consists of raw powder and a mixture. The raw powder comprises the following components in percentage by weight: 30-60 percent of a component A, 30-60 percent of a component B and 5-10 percent of a component C, wherein the component A is cassava starch; the component B is one or a mixture of broken rice meal, corn starch and soybean meal; and the component C is one or a mixture of chestnut powder, yam flour, beautiful millettia root powder and banana meal. The mixture is a proper amount of edible oil, gelatin and edible alcohol. The cassava rice takes the cassava starch as a main raw material, is additionally provided with other side crops, the chestnut powder, the yam flour, the beautiful millettia root powder and the like an auxiliary mode, is convenient to eat, reasonable in formula, fine and smooth in mouthfeel, is easily cured and less prone to breakage in a cooking process, is enriched in vitamins, amino acid and trace metal elements, can improve the nutritive value of cassava and make non-food crops become principle foods, does not have odor and is neat in appearance and good in mouthfeel.

The invention discloses a traditional Chinese medicine for treating neurodermatitis. The traditional Chinese medicine for treating neurodermatitis comprises: by weight, 30 to 40 parts of Chinese angelica, 30 to 50 parts of divaricate saposhnikovia root, 20 to 30 parts of fineleaf schizonepeta herb, 30 to 40 parts of rehmannia root, 30 to 40 parts of radix sophorae flavescentis, 15 to 25 parts of periostracum cicadae, 20 to 30 parts of rhizoma atractylodis, 20 to 30 parts of rhizoma anemarrhenae, 20 to 30 parts of millettia speciosa root, 20 to 30 parts of cortex phellodendri, 20 to 30 parts of caulis lonicerae, 20 to 30 parts of broom cypress fruit, 30 to 40 parts of gypsum, 20 to 30 parts of leapedeza bicolor. turcz. and 30 to 40 parts of talcum, wherein Chinese angelica is utilized as a monarch drug; divaricate saposhnikovia root and fineleaf schizonepeta herb are utilized as minister drugs; rehmannia root, radix sophorae flavescentis, periostracum cicadae and rhizoma atractylodis are utilized as assistant drugs; and rhizoma anemarrhenae, millettia speciosa root, cortex phellodendri, gypsum, caulis lonicerae, broom cypress fruit, leapedeza bicolor. turcz. and talcum are utilized as guide drugs. The traditional Chinese medicine for treating neurodermatitis has the advantages of convenience of preparation and use, safety, no side effect and low treatment cost. Therefore, the traditional Chinese medicine for treating neurodermatitis is an ideal drug for treating neurodermatitis.

The invention discloses millettia speciosa champ instant tea which comprises the following components in parts by weight: 80-120 parts of millettia speciosa champ dry paste powder, 550-650 parts of powdered sugar, 250-350 parts of maltodextrin, 10-30 parts of dwarf lilyturf turber dry paste powder, or 1-10 parts of chrysanthemum dry paste powder, or simultaneously comprises 10-30 parts of dwarf lilyturf turber dry paste powder and 1-10 parts of chrysanthemum dry paste powder. The millettia speciosa champ instant tea takes the traditional Chinese extract as a main component and is concentrated in nutritive and health-care components and favorable in mouthfeel and solubility; and meanwhile, the millettia speciosa champ instant tea is convenient for carrying and drinking.

The invention discloses a making method of a radix millettiae speciosae candy. The making method is characterized in that the radix millettiae speciosae candy is prepared from the following raw materials in parts by weight: 6-7 parts of radix millettiae speciosae, 2-3 parts of flos farfarae, 3-4 parts of chrysanthemum, 0.5-1 part of ginger, 3-4 parts of lucid ganoderma, 2-3 parts of grapestone extract, 200-300 parts of bee pollen and 110-118 parts of moringa pulp. Being prepared by adding traditional Chinese medicines in homology of medicine and food on the basis of the traditional hard candy, the radix millettiae speciosae candy has the advantages of unique taste, cough prevention and curing, face and skin beautifying and the like.

The invention discloses a tissue culture method for inducing and proliferating hairy roots of beautiful millettia roots. By using cotyledons of beautiful millettia roots as explants to induce hairy roots, the method comprises the following steps: sterilizing the explants; carrying out activated culture of agrobacterium; carrying out induced culture of the hairy roots; carrying out proliferative culture and the like. The method disclosed by the invention has the advantages that (1) the cotyledons of beautiful millettia roots are taken as explants for conversion; (2) the pre-culture time, concentration of bacterium, infecting time and co-culture time suitable for inducing the hairy roots of beautiful millettia roots are determined; (3) the optimal bacteriostatic agent for bacteriostatic clture of the explants is determined; (4) the optimal pre-culture, co-culture, bacteriostatic-culture and sub-culture proliferation media are determined; and (5) the whole set of method disclosed by the invention is simple and convenient to operate, low in production cost, high in induction success rate and quick in proliferation rate, can be used for obtaining the hairy roots of beautiful millettia roots and culturing the hairy roots of beautiful millettia roots on a large scale so as to extract main chemical components such as polysaccharide of beautiful millettia roots, and has very good application prospect.

The invention belongs to the technical field of agricultural planting, and particularly relates to a beautiful millettia root cultivation method which particularly includes the steps: (1) field preparation; (2) seedling culture; (3) transplantation; (4) management. Seedlings are cultured through seeds, the germination rate of the seeds is increased by the aid of compound sodium nitrophenolate solution in the seedling culture process, slurperons are implanted among beautiful millettia root nursery stocks during transplantation, the insect pest rate of the nursery stocks is decreased, ethephon solution is sprayed at the flowering stage of beautiful millettia roots to inhibit growth of flowers, and fertilizers are applied to improve the yield of the beautiful millettia roots.

The invention discloses a planting method for interplanting dragon fruits with millettia speciosa. The method includes the steps of soil preparation, determination of planting specifications, seedlingtreatment, determination of a planting method, determination of planting time, determination of a fertilization method, field management and the like for planting the dragon fruits and millettia speciosa. According to the planting method, short stem crops are suitable for being planted between dragon fruit rows, and introduction of millettia speciosa better solves the problem that the row spacingas an interplanting space is large; the roots of the dragon fruits in surface soil is in the range of 20 cm, so that the depth of an absorption area is not very high; potato blocks of millettia speciosa grow downwards and make full use of the soil layer, upper and lower structures are not contradictory or are very suitable for growth of both the dragon fruits and millettia speciosa so that not only can the time for getting benefits be shortened, but also there are other benefits. In addition, millettia speciosa is planted between the dragon fruit rows so that not only can the loss of water and fertilizer in an orchard be reduced, but also growth of weeds can be prevented, and the two crops use the space for interplanting at different times, so that the field yield is increased, and the production and income are increased.

The invention belongs to the technical field of agricultural planting. A cultivation method of millettia speciosa champ comprises the following steps: a. collecting and treating seeds; b. carrying out tissue culture; c. sowing and breeding seedlings; d. transplanting; e. carrying out water and fertilizer management; f. pruning the seedlings. The cultivation method of the millettia speciosa champ takes the seeds as a material and realizes tissue culture speed propagation of the millettia speciosa champ in a way of bud propagation; the seedlings are obtained by the steps of primary culture, proliferative secondary culture, strong seedling cultivation and rooting culture; the obtained seedlings do not have the phenomenon of dehydration. The millettia speciosa champ cultivated by the method is good in growth and development, high in plant height and strong as well as developed in seedling root system, has thick and strong rattans, and is rapid in growth, more in tuberization, easy to manage and high in disease resistance.

The invention belongs to the field of plant cultivation and relates to a millettia speciosa artificial cultivation method. The millettia speciosa artificial cultivation method includes: soaking acquired millettia speciosa seeds in compound sodium nitrophenolate solution prior to sowing the soaked seeds into nutrition bags, and transplanting millettia speciosa to a field after the seeds bud; and after the field is ripped, applying ferment organic fertilizers as base fertilizers, erecting a rattan post beside each seedling, keeping the field moist, guaranteeing nutrition supply, keeping 4-7 flower buds in the front of a flowering branch, removing rest flower buds in the back of the flowering branch, and spraying fruit retaining agents and insecticide and bactericide after the flower buds bear fruit. The millettia speciosa artificial cultivation method is simple in process, is capable of shortening growth cycle of the millettia speciosa without influencing medicinal value of the millettia speciosa and by means of sprouting and field management and increasing seed fruit bearing rate and provides a guarantee for large-area artificial cultivation of the millettia speciosa.

The invention discloses a millettia speciosa champ polysaccharide, a preparation method and application thereof in antibacterial aspect. The method includes the steps of: (1) cleaning millettia speciosa champ root tubers, performing slicing, drying, crushing and sieving, and then carrying out hot water reflux extraction to obtain a millettia speciosa champ polysaccharide crude extracted liquid; (2) removing pigment from the millettia speciosa champ polysaccharide crude extracted liquid by macroporous resin, removing residual protein by a Sevag reagent, and then conducting alcohol precipitationto obtain millettia speciosa champ crude polysaccharide; and (3) adding water into the millettia speciosa champ crude polysaccharide to prepare a millettia speciosa champ crude polysaccharide solution, then adding the millettia speciosa champ crude polysaccharide solution into a DEAE-52 cellulose anion exchange column, sequentially conducting elution with distilled water and a 0.2mol / L NaCl solution, and respectively collecting eluent to obtain millettia speciosa champ polysaccharides MSCP1 and MSCP2. The millettia speciosa champ polysaccharide prepared according to the invention has certaininhibition effect on gram-negative bacteria, especially escherichia coli and salmonella, therefore the millettia speciosa champ polysaccharide can be used for developing antibacterial materials or antibacterial agents.

The invention relates to Radix Millettiae Speciosae-Morinda citrifolia compound enzyme and a preparation method thereof and an application thereof in the preparation of a facial mask. The preparationmethod of the Radix Millettiae Speciosae-Morinda citrifolia compound enzyme comprises the following steps: (1) preparing Morinda citrifolia enzyme: chopping fresh Morinda citrifolia fruits, putting the chopped Morinda citrifolia fruits into a sealed sterile tank, and performing natural fermentation at room temperature for one year to obtain the Morinda citrifolia enzyme; and (2) preparing the Radix Millettiae Speciosae-Morinda citrifolia compound enzyme: chopping fresh Radix Millettiae Speciosae roots, uniformly mixing the chopped Radix Millettiae Speciosae roots with the Morinda citrifolia enzyme prepared in the step (1), putting the mixture into a sealed sterile tank, and performing natural fermentation at room temperature for one month to obtain the Radix Millettiae Speciosae-Morinda citrifolia compound enzyme.

The invention discloses a health-preserving millettia speciosa-Chinese wolfberry fruit tea. The health-preserving millettia speciosa-Chinese wolfberry fruit tea is composed of millettia speciosa, morinda officinalis, liquorice roots and Chinese wolfberry fruits. The preparation technology of the health-preserving millettia speciosa-Chinese wolfberry fruit tea comprises the following steps: (1), mixing the morinda officinalis, the liquorice roots and the Chinese wolfberry fruits, soaking the mixture in water, decocting the mixture over slow fire for 1-2 hours, filtrating the decoction, and obtaining a filtrate; (2), adding water into the filter residues, decocting the mixture over slow fire for 1-2 hours, filtrating the decoction, obtaining a filtrate, combining the filtrates, and carrying out concentration; (3), adding the millettia speciosa into the concentrated filtrate, stewing the mixture away from fire until the filtrate is completely absorbed by the millettia speciosa, and stopping stewing; (4), drying the millettia speciosa, and stir-frying the dried millettia speciosa until the outer surfaces of the millettia speciosa are golden in color, so that the health-preserving millettia speciosa-Chinese wolfberry fruit tea is prepared. The health-preserving millettia speciosa-Chinese wolfberry fruit tea disclosed by the invention has good effects of resisting oxidation, enhancing immunity, nourishing the kidney, invigorating Yang, strengthening the tendons, reinforcing the bones, expelling wind and removing dampness; moreover, the health-preserving millettia speciosa-Chinese wolfberry fruit tea is obvious in healthcare effects and free of side effects after drinking. The preparation technology disclosed by the invention is capable of more effectively amplifying functions of the health-preserving millettia speciosa-Chinese wolfberry fruit tea; and the prepared health-preserving millettia speciosa-Chinese wolfberry fruit tea is convenient to carry and drink as well as low in production cost; and the technological processes are simple and easy to operate.

The invention discloses a preparation method of health-care mooncakes. The preparation method comprises the following steps of preparing an extracting solution, preparing mooncake filling, preparing ice skins, preparing ice skin mooncakes and the like. According to the preparation method disclosed by the invention, beautiful millettia root and an extracting solution obtained by boiling traditional Chinese medicinal materials are used for preparing the mooncake filling, and beautiful millettia powder and glutinous rice flour are used for preparing the ice skins, thereby preparing the health-care mooncakes for tonifying deficiency and moistening lung.

The invention discloses a preparation method and use of polysaccharide of radix millettiae speciosae, relates to a natural product polysaccharide, and aims to solve the problems of low yields and unsatisfied purities as required for drugs and health care product additives for extraction processes of polysaccharide of radix millettiae speciosae in the prior art. The preparation method comprises thefollowing steps: (1) subjecting radix millettiae speciosae powder to supercritical CO2 extraction to obtain a radix millettiae speciosae extract; (2) soaking the extract in water of equal weight, wherein a complex enzyme accounting for 0.5-1.0% of the raw materials by mass is added to the water, and proportions of the components in the complex enzyme are as follows: cellulase:xylanase:protease =(1-3):(3-1):(2-4); (3) subjecting the enzymolysis mixture from the step (2) to continuous countercurrent extraction with water to obtain an extract; (4) after filtering the extract to remove impurities, adsorbing the extract by using a macroporous resin, filtering the extract, retaining the entrapped liquid, and concentrating the entrapped liquid in vacuum to 10-20% of the original volume; and (5)drying: drying the entrapped concentrate, thereby obtaining the polysaccharide of radix millettiae speciosae. The polysaccharide of radix millettiae speciosae obtained by the preparation method can used in production of medicaments for treating acute lung injury.

The invention relates to a pharmaceutical composition for treating liver cirrhosis and a preparation method of the pharmaceutical composition. The pharmaceutical composition is prepared from the following traditional Chinese medicine raw materials in parts by weight: kadsura coccinea, erythrina variegate, radix ilicis asprellae, golden cypress, ovate leaf holly bark, arillus longan parasite, pyrus pyrifolia parasite, water banyan parasite, buddleja asiatica, radix millettiae speciosae, root of arrowleaf abelmoschus, rhizome of perny false fairybells, livistona chinensis, alpinia speciosa, Xiaochangfeng (a medicine for treating hernia), kadsura pepper stem, pericarpium citri reticulatae, salvia miltiorrhiza, cablin potchouli herb, rhizoma cyperi, gynostemma pentaphyllum and herba selaginellae doederleinii. With application of the pharmaceutical composition, various symptoms of patients with the liver cirrhosis can be effectively relieved.

The invention belongs to the technical field of plant tissue culture, and discloses a tissue culture method for increasing the survival rate of seed seedlings of millettia speciosa champ. The tissue culture method comprises five steps of sterilization of an explants, primary culture, secondary culture, rooting culture and seedling hardening. According to the tissue culture method disclosed by theinvention, the sterilization of the explants is carried out by adopting a plant treatment solution, chemical reagents such as mercury bichloride are prevented from being used, the environment is protected, and cultured seed seedlings of the millettia speciosa champ are strong in adaptability and higher in survival rate.