The invention discloses an anti-oxidation moisturizing sleeping mask containing peony seed oil and a preparation method thereof.The sleeping mask is prepared from, by mass, 0.5%-20% of peony seed oil, 0.1%-5% of oil-soluble vitamin E, 0.1%-5% of an emulsifier, 1%-10% of rose hydrosol, 0.5%-20% of chamomile extract, 1%-10% of glycerin, 0.5%-3% of lower-molecular hyaluronic acid, 5%-15% of aloe gel, 0.05%-5% of an antibacterial agent and the balance deionized water.After peony seed oil, rose hydrosol, chamomile extract, aloe gel, lower-molecular hyaluronic acid and other substances are mixed, the sleeping mask suitable for modern women is formed; the sleeping mask has the advantage of being free of washing, and thus use is quite convenient.With natural substances serving as main raw materials, the mask has the advantages of being mild without irritation and easy to absorb and is high in moisturizing performance and good in anti-oxidation effect; after the mask is used, the skin is smooth and fine, moist and compact, crystal clear and full of vitality.