Epigallo-catechin gallate (EGCG) with high purity and preparation method thereof
A technology of epigallocatechin and gallate, which is applied in the field of pharmaceutical compositions with epigallocatechin gallate as the main component, epigallocatechin gallate and its preparation, can solve technical problems Difficulty in large-scale industrial production, inability to carry out industrial production, and inability to use industrial production to achieve good market advantages, low production costs, and good stability
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Embodiment 1
[0035] Take 10 kg of crude tea polyphenols (with an EGCG content of 40.4%), add 100 L of purified water, soak and stir at 45°C for extraction twice, each time for 0.5 hours, combine the extracts, filter, and discard the residue;
[0036] After the extract was concentrated to 50L under reduced pressure at 60°C, 50L ethyl acetate was added for liquid-liquid extraction twice, the ethyl acetate layer was combined and recovered under reduced pressure at 60°C to a thick paste, and 50L purified water was added to dissolve to obtain tea Polyphenol sample solution.
[0037] Pass the above tea polyphenol sample solution through a tandem chromatographic column equipped with 100-200 mesh polyamide (front column) and HPD-100 macroporous adsorption resin (back column), add 100L of purified water to fully elute, and remove In series, add 100L of 20% ethanol to the back column (HPD-100 macroporous adsorption resin column) for desorption and elution, collect the eluate, and obtain an epigalloc...
Embodiment 2
[0041] Take 10 kg of crude tea polyphenols (with an EGCG content of 45.1%), add 50 L of ethanol (concentration: 10%), soak and stir at 45°C for 3 times, each time for 1 hour, combine the extracts, filter, and discard the residue;
[0042] After the extract was concentrated to 50L under reduced pressure at 55°C, 50L ethyl acetate was added for liquid-liquid extraction three times, the ethyl acetate layers were combined and recovered under reduced pressure at 50°C to a thick paste, and dissolved in 100L purified water to obtain tea Polyphenol sample solution.
[0043] Pass the above-mentioned tea polyphenol sample solution through a tandem chromatography column equipped with 30-100 mesh polyamide (front column) and D101 macroporous adsorption resin (rear column) respectively, add 80L of purified water for sufficient elution, and release the tandem column. Add 70L of 30% ethanol to the back column (D101 macroporous adsorption resin column) for desorption and elution, and collect ...
Embodiment 3
[0047] Take 50 kg of crude tea polyphenols (with an EGCG content of 35.4%), add 300 L of purified water, soak and stir at 40°C for extraction twice, each time for 1 hour, combine the extracts, filter, and discard the residue;
[0048] After the extract was concentrated to 300L under reduced pressure at 65°C, 300L ethyl acetate was added for liquid-liquid extraction twice, the ethyl acetate layer was combined and recovered under reduced pressure at 50°C to a thick paste, and 600L purified water was added to dissolve to obtain tea Polyphenol sample solution.
[0049] The above-mentioned tea polyphenol sample solution is passed through the tandem chromatographic columns respectively equipped with model HPD-100 macroporous adsorption resin (front column) and D101 macroporous adsorption resin (back column), after adding 500 L of purified water to fully elute, Remove the series connection, add 400L of 25% ethanol to the back column (D101 macroporous adsorption resin column) for deso...
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