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Bacillus subtilis, screening method thereof, application of bacillus subtilis, biological preservative, preparation method thereof and fruit and vegetable preserving method

A technology of Bacillus subtilis and biological preservatives, applied in the field of bioengineering, can solve the problems of not obvious respiratory inhibition effect, no effective antiseptic effect, and reduced antiseptic effect, and achieve wide range of action, strong inhibitory effect, and good heat resistance Effect

Inactive Publication Date: 2016-11-23
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But it only has significant antagonism effect to pear postharvest pathogen-black spot, does not have the effect of effectively antiseptic to other perishable fruits, and it has the shortcoming that broad spectrum is not good, simultaneously this bacterial strain is to the fruit and vegetable produced The respiratory inhibition effect is not obvious, which further reduces the antiseptic effect

Method used

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  • Bacillus subtilis, screening method thereof, application of bacillus subtilis, biological preservative, preparation method thereof and fruit and vegetable preserving method
  • Bacillus subtilis, screening method thereof, application of bacillus subtilis, biological preservative, preparation method thereof and fruit and vegetable preserving method
  • Bacillus subtilis, screening method thereof, application of bacillus subtilis, biological preservative, preparation method thereof and fruit and vegetable preserving method

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Embodiment 1

[0034] Embodiment one: a kind of bacillus subtilis, described bacillus subtilis is bacillus subtilis (Bacillus subtilis) Cy-29, is preserved in the Microbiology Center of China Committee for Culture Collection of Microorganisms, and is preserved in China General Microbiology Culture Collection Management Center, The date of deposit is July 12, 2013, and the deposit number is CGMCC 7916.

[0035] The present invention extracts and screens out a kind of Bacillus subtilis Cy-29 from the soil, which is originally derived from a soil sample of an orchard. The sample is serially diluted, separated and purified to obtain a strain that has an inhibitory effect on plant pathogenic fungi, and The strains were identified. Bacillus subtilis (Bacillus subtilis) Cy-29 and the antibacterial substances produced by its fermentation have a strong broad-spectrum antifungal effect, and have a good inhibitory effect on a variety of plant growth pathogenic fungi and fruit and vegetable storage fung...

Embodiment 2

[0042] Embodiment two: a kind of Bacillus subtilis, described Bacillus subtilis is Bacillus subtilis (Bacillus subtilis) Cy-29, is preserved in China Common Microorganism Culture Preservation Management Center, preservation date is July 12, 2013, preservation number for CGMCC 7916. The cells of Bacillus subtilis (Bacillus subtilis) Cy-29 are short rod-shaped, Gram staining is positive, and spores exist. On nutrient agar, colonies appear rough, low raised, with irregular edges, matt and opaque.

Embodiment 3

[0043] Embodiment three: a kind of Bacillus subtilis, described Bacillus subtilis is Bacillus subtilis (Bacillus subtilis) Cy-29, preserved in China Common Microbial Strain Preservation Management Center, preservation date is July 12, 2013, preservation number for CGMCC 7916. The cells of Bacillus subtilis (Bacillus subtilis) Cy-29 are short rod-shaped, Gram staining is positive, and spores exist. On nutrient agar, colonies appear rough, low raised, with irregular edges, matte and opaque. The 16S rDNA sequence of the Bacillus subtilis is 1589bp long, and its sequence is shown in SEQ ID No.1.

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Abstract

The invention relates to the technical field of biological engineering, in particular to a bacillus subtilis, a screening method thereof, an application of the bacillus subtilis, a biological preservative, a preparation method thereof and a fruit and vegetable preserving method. The bacillus subtilis Cy-29 is collected in China General Microbiological Culture Collection Center on December 7th, 2013, and the collection number of the bacillus subtilis is CGMCC 7916. The biological preservative comprises the bacillus subtilis Cy-29. The fruit and vegetable preserving method includes a method for fermenting a product by the bacillus subtilis Cy-29. By planting and growth of the bacillus subtilis Cy-29 and / or antagonism of antibacterial substances generated by the bacillus subtilis Cy-29, respiration of fruits is effectively inhibited, the rotting rate of the fruits is obviously reduced during cold storage, and strong inhibiting effects to various plant pathogenic fungi are achieved.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a bacillus subtilis, its screening method and use, a biological preservative, its preparation method, and a fruit and vegetable antiseptic method. Background technique [0002] In agricultural production, plant diseases are one of the main causes of crop yield loss and quality degradation, most of which are caused by fungi. According to reports, 10%-30% of fresh fruits are lost to post-harvest rot every year in developed countries, while in developing countries that lack refrigeration storage and transportation facilities, the fruit rot rate exceeds 40%, and most of the fruit rots are caused by fungi. In the actual production process, plant disease control mainly depends on chemical pesticides, which play an important role in protecting agricultural production. However, its long-term widespread use will lead to a series of problems such as pathogenic bacteria developing d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00A23B7/155A23B7/154C12R1/125
CPCA01N63/00A23B7/154A23B7/155A01N63/10C12N1/20C12N1/205C12R2001/125
Inventor 胡欣洁
Owner SICHUAN AGRI UNIV
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