Tissue culture method for inducing and proliferating hairy roots of beautiful millettia roots

Abstract

The invention discloses a tissue culture method for inducing and proliferating hairy roots of beautiful millettia roots. By using cotyledons of beautiful millettia roots as explants to induce hairy roots, the method comprises the following steps: sterilizing the explants; carrying out activated culture of agrobacterium; carrying out induced culture of the hairy roots; carrying out proliferative culture and the like. The method disclosed by the invention has the advantages that (1) the cotyledons of beautiful millettia roots are taken as explants for conversion; (2) the pre-culture time, concentration of bacterium, infecting time and co-culture time suitable for inducing the hairy roots of beautiful millettia roots are determined; (3) the optimal bacteriostatic agent for bacteriostatic clture of the explants is determined; (4) the optimal pre-culture, co-culture, bacteriostatic-culture and sub-culture proliferation media are determined; and (5) the whole set of method disclosed by the invention is simple and convenient to operate, low in production cost, high in induction success rate and quick in proliferation rate, can be used for obtaining the hairy roots of beautiful millettia roots and culturing the hairy roots of beautiful millettia roots on a large scale so as to extract main chemical components such as polysaccharide of beautiful millettia roots, and has very good application prospect.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, in particular to a tissue culture method for inducing and proliferating bovine hairy roots. Background technique [0002] Niu Dali is a plant of the genus Millettia speciosa Champ. in the leguminous family, also known as Dali potato and mountain lotus root. ) local medicinal materials. The root of Niu Dali is used as medicine, which is sweet and flat. Return lung, kidney channel. It has the effect of tonifying deficiency and nourishing the lungs, strengthening tendons and activating collaterals. It has been clinically proven to have good curative effect on chronic diseases such as lumbar muscle strain, rheumatoid arthritis, lung deficiency cough, tuberculosis, chronic bronchitis, and chronic hepatitis. The folks of the Zhuang people are often used for the treatment of lumbago and leg pain, fever (rheumatic bone pain), tuberculosis, chronic hepatitis, and chronic gastritis. [0003] M...

AI Technical Summary

Problems solved by technology

In order to solve the contradiction between supply and demand, people try to expand the drug source by artificial cultivation. Some successful research reports have been found so far, but there are still many problems restricting its large-scale production. It is very unstable, the survival rate of cutting seedlings is low, and it is very difficult for tissue culture seedlings to take root, etc.

Moreover, the growth cycle of cattle vigorously is long (generally 3-4 years before the production is formed), and it takes a long time to breed or raise seedlings by conventional methods. Due to the small reproduction coefficient and large seed consumption, the development speed is very slow and the production cost increases. Moreover, serious degradation due to damage by diseases and insect pests has seriously affected the yield and quality of medicinal materials.