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A kind of mixed bacteria producing biosurfactant and its screening method

A surfactant and biological surface technology, which is applied in the field of microorganisms for oil recovery in oil fields, can solve problems such as no breakthroughs, and achieve the effects of reducing screening costs, wide application, and strong selectivity

Inactive Publication Date: 2018-08-07
SUZHOU ZFA NEW ENERGY TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, how to screen mixed bacteria that produce biosurfactants has not yet made a breakthrough

Method used

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  • A kind of mixed bacteria producing biosurfactant and its screening method
  • A kind of mixed bacteria producing biosurfactant and its screening method
  • A kind of mixed bacteria producing biosurfactant and its screening method

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Embodiment Construction

[0042] Below in conjunction with the examples, the specific implementation of the present invention will be further described in detail. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0043] One, the screening method of the mixed bacteria that produces biosurfactant of the present invention, comprises the following steps:

[0044](1) Activation of bacterial strains: Transfer the strains to be screened for oil production to the slant medium, and cultivate them at 37°C for 2 days, wherein the slant medium is: 3g of beef extract, 10g of peptone, 5g of sodium chloride, 20g of agar, 1000mL of distilled water, pH 7.0, steam sterilization at 121°C for 20 minutes, the oil production strains to be screened in this specific example include the preservation number CCTCC AB 205091 and the name Pseudomonas stutzeri (Pseudomonas stutzeri), the preservation number is CCTCC S2013441 and the name It is a...

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Abstract

The invention relates to a mixed bacteria producing biosurfactant and a screening method thereof. The mixed bacteria is composed of three strains, named Pseudomonas stutzeri (Pseudomonas stutzeri) and the preservation numbers are CCTCC AB 205091, Composition of strains named Nocardioides ginsengagri with preservation number CCTCC S2013441 and Bacillus licheniformis with preservation number CCTCC AB 205141. 1. Activate and cultivate the selected oil production strains to be screened, conduct preliminary screening on the activated and cultured oil production strains to be screened by the blood plate method to obtain the primary screening fermentation liquid, and use the oil discharge ring method to re-screen the primary screening fermentation liquid after filtering. The mixed bacteria combines the advantages of the three strains and can produce biosurfactants used in oil recovery. The screening method of the mixed bacteria is widely applicable and effectively reduces the screening cost. In addition, the screening method has high accuracy and selectivity. Strong, convenient and easy-to-operate process, and compared with the prior art, the cycle is shorter.

Description

technical field [0001] The invention relates to a microorganism used for oil recovery in an oil field, in particular to a mixed bacteria producing biosurfactant and a screening method thereof. Background technique [0002] Enhanced oil recovery is a kind of production technology that enhances oil recovery by improving the physical and chemical properties of oil reservoirs and oil reservoir fluids. Oil recovery technology can be roughly divided into primary oil recovery, secondary oil recovery and tertiary oil recovery. The primary oil recovery is carried out by using the natural energy in the oil layer; the secondary oil recovery is to use certain technology to restore the pressure of the oil layer to carry out oilfield development. The common ones are Oil recovery by water flooding; after that, the technology of enhanced oil recovery belongs to tertiary oil recovery. At present, except the Middle East, the main oil fields of the world's major oil-producing countries have e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12Q1/04C12P19/44C09K8/584C12R1/38C12R1/10C12R1/01
CPCY02P20/59
Inventor 段浩
Owner SUZHOU ZFA NEW ENERGY TECH CO LTD
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