Functional bacterium applicable to petroleum polluted soil repairing in positive column of direct current electric field and application thereof

A direct current electric field, polluted soil technology, applied in the field of soil environmental remediation, can solve the problems of weakening the overall remediation efficiency of electrokinetic enhanced microbial remediation technology, adverse effects on microbial abundance and activity, and accumulation of soluble inorganic ions, etc. Adaptability, make up for the lack of functional microorganisms, and improve the effect of application efficiency

Active Publication Date: 2020-07-24
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, not all oil-degrading functional microorganisms are able to adapt to the electric field conditions in the direction of the DC constant field strength, especially in the anode area of ​​the electrokinetic restoration area, where there is an environment of extreme acid and high redox potential. Due to the direction of the constant field strength of direct current, the content of soluble inorganic ions in the pole area gradually accumulates and increases, even reaching the level of saline soil, and some special ions such as chloride ions are oxidized by the anodic area. Oxidation products have a certain Microbial growth inhibition, these factors have obvious adverse effects on the abundance and activity of microorganisms in the anode area, thus weakening the overall remediation efficiency of electrodynamically enhanced microbial remediation technology to a certain extent

Method used

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  • Functional bacterium applicable to petroleum polluted soil repairing in positive column of direct current electric field and application thereof
  • Functional bacterium applicable to petroleum polluted soil repairing in positive column of direct current electric field and application thereof
  • Functional bacterium applicable to petroleum polluted soil repairing in positive column of direct current electric field and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Isolation, Screening and Identification of Variovorax sp.JWLB3 (Variovorax sp.JWLB3)

[0034] The source samples of the functional strains come from the long-term oil-contaminated soil around the oil well in an oilfield area. After the soil samples are collected, they are domesticated in a DC electric field. The domestication process is completed in a box with a length of 26 cm, a width of 12 cm, and a height of 10 cm. The box soil A direct current with a voltage difference of 24V is connected to the interior, and water is regularly replenished in the anode area of ​​the direct current electric field during the domestication process, and the initial concentration of petroleum hydrocarbons in the soil reaches 68g·kg -1 . The domestication period is 45 days, and the microorganisms in the oil-contaminated soil domesticated by the DC electric field are screened and cultivated in the inorganic salt screening medium for 10 days, wherein the total oil content in the ...

Embodiment 2

[0041] Example 2 Remediation of Variovorax sp.JWLB3 (Variovorax sp.JWLB3) to Petroleum Hydrocarbon Contaminated Soil under DC Electric Field Conditions

[0042] Inoculate JWLB3 (Variovorax sp.JWLB3) in beef jerky peptone medium, adjust the pH of the medium to 3.5, culture at 30°C and 180rpm for 3 days, and centrifuge at 8000rpm to collect the bacteria. The buffer solution was used to wash the bacteria, and the bacteria were resuspended in the aforementioned inorganic salt medium to prepare a bacteria suspension of Variovorax sp. JWLB3 bacteria. The oil-contaminated soil to be treated is sterilized by high temperature and high pressure steam to eliminate the indigenous microorganisms in the soil. The bacterial suspension of Variovorax sp.JWLB3 was inoculated in the sterilized contaminated soil with an oil content of 4.6%, and the bacterial microbial abundance in the treated soil after inoculation reached 3.54×10 8 CFU / g dry soil. The oil-contaminated soil mixed with functiona...

Embodiment 3

[0046] Example 3 The combined application of Variovorax sp.JWLB3 (Variovorax sp.JWLB3) and other functional bacteria to the remediation of petroleum hydrocarbon contaminated soil under DC electric field conditions

[0047] According to the record of the culture mode in embodiment 2 and the prior art, respectively aim at the phage genus JWLB3 (Variovoraxsp.JWLB3), Pseudomonas fluorescens (Pseudomonas fluorescens), Bacillus cereus (Bacillus cereus) and Bacillus licheniformis ( Bacillus licheniformis) were cultivated for use.

[0048] The bacterium solution of Variovorax sp.JWLB3 was mixed into the soil in the anode area, which accounted for 1 / 3 of the total length of the experimental device, and Pseudomonas fluorescens, Bacillus cereus and Bacillus licheniformis The bacillus (Bacillus licheniformis) was mixed into the soil in the middle zone and the cathode zone, which accounted for 2 / 3 of the total length of the experimental device.

[0049] The oil content of the polluted soi...

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Abstract

The invention belongs to the field of soil environment repairing, further relates to organic pollutant electric repairing and microbial repairing synergistic treatment technology and particularly relates to a functional strain applicable to petroleum polluted soil repairing in the positive column of a direct current electric field. The strain is Variovorax JWLB3 (Variovorax sp.JWLB3), is preservedin the China Center for Type Culture Collection on October 11, 2019, and has a preservation access number of CCTCC NO:M 2019811. The Variovorax JWLB3 is used for combining with the electric repairingtechnology and repairing petroleum polluted soil in each area applied by the direct current electric field, especially the positive column of the electric field. When a relatively proper water containing condition is kept, the functional strain can keep relatively high bioactivity under the conditions of high acidity, high soluble salt ions, high chlorine content, high redox potential and strongcurrent intensity of the electrically repaired positive column, and keep relatively good petroleum hydrocarbon degradation performance.

Description

technical field [0001] The invention belongs to the field of soil environment remediation, and in particular relates to the synergistic treatment technology of electrodynamic remediation and microbial remediation of organic pollutants, and specifically relates to a functional bacterial strain suitable for remediation of oil-contaminated soil in the anode area of ​​a DC electric field and its application. Background technique [0002] With the continuous development of the petroleum industry, the exploitation and demand of petroleum fossil fuels are increasing day by day, and the ensuing oil pollution problem is becoming more and more prominent. Oil-contaminated soil has become a hot and difficult issue in the environmental field. Various technical forms have been developed for the remediation of oil-contaminated soil, but each has its advantages and disadvantages. Taking into account restoration costs, economic benefits, ecological security, and restoration effects, microbia...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/08B09C1/10C12R1/01C12R1/39C12R1/085C12R1/10C12R1/385C12R1/32
CPCC12N1/20B09C1/085B09C1/10C12R2001/01C12N1/205
Inventor 郭书海王卅程凤莲吴波李凤梅
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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