Arylsulfatase and preparation method and applications thereof

Abstract

An arylsulfatase and a preparation method and applications thereof, relate to an arylsulfatase with a new gene sequence and preparation thereof. Provided is arylsulfatase Ary423 originated from the Flammeovirga Pacifica H2. Preparation methods of arylsulfatase gene Ary423 and arylsulfatase Ary423 are also provided. Gene Ary423 is cloned, recombinant plasmid carrying with the gene Ary423 is constructed and then converted to the escherichia coli BL21, the positive clones are selected and cultured in a culture medium, then the cultured positive clones are re-suspended in a lysis buffer, after the processes of cracking and centrifugation, the supernatant is collected and homogeneously mixed and purified with Ni-NTA Agarose. Arylsulfatase Ary423 can stably express in escherichia coli, recombinase maintains high catalysis efficiency in a relatively wide temperature range and pH range, so that the shortages of low vigor of arylsulfatase and tedious processes of separating and purifying in the prior art are overcome.

Description

technical field [0001] The invention relates to an arylsulfatase with a new gene sequence, a preparation method thereof, and an application thereof in an agar gel extraction process. Background technique [0002] Agar gum is a natural polysaccharide colloid extracted from red algae seaweed, which is widely used in many aspects such as food industry, pharmaceutical industry, daily chemical industry, and biological engineering. Gel strength is the main index to measure the quality of agar products, and the sulfate group is the key factor that hinders the formation of agar gel molecules. In the process of producing agar, the crude agar molecules in seaweed have a high content of sulfate groups, which need to be treated with alkali (NaOH) to reduce the sulfate groups (SO 4 2- ) content, thereby improving the gel strength of agar products, so alkali treatment is an essential process in the industrial production of agar. In the alkaline treatment process, about 400kg of NaOH is...

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Problems solved by technology

In 2004, Lim JM et al. (Lim JM, Jang YH, Kim HR, Kim JK, Nam SW. Overexpression of arylsulfatase in E.coli and its application to desulfation of agar, J Microbiol Biotechnol.2004,14:777–782.) It was found that the arylsulfatase from Pseudoalteromonas carrageenovora ATCC43555 strain can degrade the substrate p-nitrophenyl sulfate (pNPS), and its gene can degrade SO on agar after expression in E. coli 4 2- The ability to increase the gel strength and decrease the conductivity of agar

At present, there is no patent on arylsulfatase, and there is no patent on sulfatase related to the treatment of agar.

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