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Strain belonging to genus mitsuaria and microbial pesticide using said strain

A technology of Songjiang bacteria and strains is applied in the field of plant growth regulators and bacterial plant disease control agents, which can solve the problems of inability to establish and use biological pesticide control methods, insufficient control effects, and unclear effectiveness.

Active Publication Date: 2020-05-01
GIFU UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With regard to R. solanacearum, control by using microbial pesticides of strains of the genus Pseudomonas, Ralstella, Acinetobacter, and Pythium has been studied so far ( For example, see Non-Patent Document 1 for details on pesticides using Acinetobacter GEBT349 strain, but even these biopesticides have insufficient control effects
In addition, attempts have been made to control bacterial wilt by injecting compost containing various microorganisms, but its effectiveness is not yet clear, and there are many cases of control failures
Furthermore, these methods require immobilization of antagonistic bacteria in the soil, rhizosphere, and plant body, and it is often difficult to show a stable effect in soil under extremely diverse conditions. In addition, the immobilization of strains that do not show pathogenicity in plants and multiplication itself are often difficult, which is one of the main reasons why it is impossible to establish a control method using biopesticides

Method used

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  • Strain belonging to genus mitsuaria and microbial pesticide using said strain
  • Strain belonging to genus mitsuaria and microbial pesticide using said strain
  • Strain belonging to genus mitsuaria and microbial pesticide using said strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] (Confirmation test 1 for the control effect of tomato bacterial wilt disease)

[0082]Tomatoes (variety: Ponderosa) were grown to the 4th compound leaf stage in plastic pots with a diameter of 9 cm (the bottom layer was 150 g of seedling soil, the middle layer was 20 g of river sand, and the upper layer was 150 g of seedling soil) filled with seedling soil. Then, after mixing 30 ml of the suspension of chitosan-solvating Matsue bacteria TWR114 strain and 70 ml of sterilized water, it was treated in a plastic tank by supplying water from the bottom, and stored in a greenhouse at 30° C. for 3 days. Thereafter, 100 ml of the suspension of Ralstonia solanacearum of tomato was inoculated by the bottom surface water supply treatment similarly. As a comparison, only the suspension of Ralstonia solanacearum of tomato was subjected to bottom surface water supply treatment (no treatment zone). It should be noted that the TWR114 strain suspension was cultured with NB medium (meat...

Embodiment 2

[0088] (Confirmation test for control effect of tomato bacterial wilt 2)

[0089] Tomatoes (variety: Ponderosa) were grown to the 4th compound leaf stage in pots (9 cm x 9 cm) filled with horticultural soil. Then, 30 ml of the TWR114 strain suspension was perfused and stored in a greenhouse at 30° C. for 4 days. Thereafter, 50 ml of tomato solanacearum suspension was inoculated by the same perfusion treatment. As a comparison, only the tomato solanacear suspension was perfused (no treatment zone) and 10 ml of Acinetobacter (Acinetobacter) GEBT349 strain suspension described in Non-Patent Document 1 was carried out by spraying the stems and leaves ( control area). It should be noted that the TWR114 strain suspension and the GEBT349 strain suspension were used in NB medium (meat extract 0.5%, peptone 1.5%, sodium chloride 0.5%, potassium monohydrogen phosphate 0.5%, pH 7.0) Centrifuge at 27°C and 120rpm for 48 hours to collect bacteria (5000×g, 15 minutes), suspend them in st...

Embodiment 3

[0104] (Confirmation test for the control effect of potato bacterial wilt)

[0105] Potatoes (variety: Dejima) were grown to the 4th compound leaf stage in plastic pots with a diameter of 9 cm filled with horticultural soil. Then, 30 ml of the TWR114 strain suspension was poured into a plastic tank, and stored in a greenhouse at 30° C. for 4 days. Thereafter, 50 ml of the potato solanacear suspension was inoculated by the same perfusion treatment. As a comparison, the operation of perfusing only the suspension of Ralstonia solanacearum (no treatment area) and the operation of spraying 10 ml of Acinetobacter GEBT349 strain suspension on stems and leaves (control area) were also implemented. It should be noted that the TWR114 strain suspension and the GEBT349 strain suspension were used in NB medium (meat extract 0.5%, peptone 1.5%, sodium chloride 0.5%, potassium monohydrogen phosphate 0.5%, pH 7.0) Centrifuge at 30°C and 120rpm for 24 hours to collect bacteria (5000×g, 10 mi...

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Abstract

Provided herein is a non plant-pathogenic strain that can stably exhibit a bacterial plant disease control effect at the actual site of crop production, and that can be safely used as a biopesticide against plant. A bacterial plant disease control agent using such a strain is also provided, among others. In the present invention, viable bacteria of a strain belonging to genus Mitsuaria, which wasnot known to have a control effect against bacterial plant disease, or a culture containing the viable bacteria of such a strain are used as an active component, and the invention provides bacterial plant disease control agents such as a vegetable wilt disease control agent, and a canker control agent.

Description

technical field [0001] The present invention relates to bacterial strains belonging to the genus Mitsuaria, agents for preventing and controlling bacterial plant diseases, plant growth regulators, and the like using the strains and the like. Background technique [0002] The prevention and control of crop diseases (plant diseases) using pathogenic bacteria is a very important issue for agricultural personnel all over the world, and measures such as the control of cultivation and the use of fungicides and disinfectants are carried out among them. However, among the bacterial plant diseases, there are some diseases that are difficult to control even with the use of such tillage control, fungicides and disinfectants. [0003] For example, bacterial wilt of vegetables is a soil-borne disease caused by Ralstonia solanacearum, a bacterium that reproduces in the soil. If tomatoes, eggplants, green peppers, potatoes, etc. are infected with the bacterial wilt, the whole plant Wither...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01C1/00A01G7/00A01N63/20A01P3/00A01P21/00
CPCA01C1/00A01G7/00C12N1/20A01N63/20C12R2001/01C12N1/205A01H6/82
Inventor 清水将文C.M.马来克铃木聪美尾崎刚一
Owner GIFU UNIVERSITY
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