Radiation resistant pantoea sp. W36-1 and application thereof in environmental engineering

Abstract

The invention discloses pantoea sp. W36-1, of which the preservation number is CGMCC No. is 3701, a flocculant prepared by the pantoea sp. W36-1 and application of the flocculant in removal of suspensions, dyeing agents and heavy metal in waste water. Extracellular products of the bacteria have an obvious absorption peak at 200 to 250nm, and polysaccharide solution can remove 54.89 percent of free groups in the bacteria. The method for preparing the pantoea sp. W36-1 comprises the following steps of: performing fermentation culture of the bacteria strain for 48 hours, centrifuging 100ml of fermentation solution at a rotation speed of 6,000r / min for 10 minutes, adding absolute ethanol, of which the volume is 1.5 times that of supernatant, into the supernatant, uniformly mixing the absoluteethanol and the supernatant standing the mixed solution at the temperature of 4 DEG C overnight, centrifuging the precipitate, washing the precipitate by 70-percent ethanol for 2 to 3 times, and thendrying the precipitate to obtain the finished product. The flocculent activity of the fermentation culture product of the pantoea sp. W36-1 is 81.7 to 83.15 percent, the yield of the pantoea sp. W36-1 is 6g / L and can be widely used for removing suspensions, dyeing agents, heavy metal and the like in waste water.

Description

technical field [0001] The invention belongs to the technical field of environmental engineering for the application of microbial strain resources. Specifically, it relates to the technical field of a new species of Pantoea and its application to environmental engineering. Background technique [0002] At present, among the technologies and methods of sewage treatment, the flocculation and sedimentation method is one of the most widely used and low-cost methods, and the key to this process is the selection and addition of flocculants with excellent performance. [0003] There are three main types of flocculants. One is low-molecular inorganic salts, such as aluminum salts and iron salts, which are used in large amounts, which can easily cause secondary pollution and affect human health; the other is synthetic polymer flocculants, which have fast flocculation speed and high efficiency, but are difficult to biodegrade. , toxic residual monomers and other issues; third, natur...

AI Technical Summary

Problems solved by technology

One is low-molecular inorganic salts, such as aluminum salts and iron salts, which are used in large amounts, which can easily cause secondary pollution and affect human health; the other is synthetic polymer flocculants, which have fast flocculation speed and high efficiency, but are difficult to biodegrade. , toxic residual monomers and other issues; third, natural organic polymer flocculants have the advantages of strong selectivity, non-toxicity, cheap price, easy degradation, no secondary pollution and regeneration, etc., and are currently the focus of flocculant research, but weak However, its flocculation activity limits its further popularization and application.

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