A method for staining apoptotic cells

Abstract

The invention provides an apoptotic cell staining method. The apoptotic cell staining method includes the steps of (1), dewaxing sections to water and staining for 0.2-5 minutes by a staining solutionI, wherein the staining solution I comprises 0.1-1 g of acid fuchsin, 0.1-2 g of ponceau, 0.1-3 g of orange G and 300ml of 0.2% acetic acid; (2), washing by an acetic acid solution, treating for 3-8minutes by a phosphomolybdic acid solution, and washing by the acetic acid solution again; (3), staining for 10-30 minutes by a staining solution II and washing by the acetic acid solution again, wherein the staining solution II comprises 0.1-1 g of brilliant green and 100 ml of 0.2% acetic acid; (4), performing dehydration and transparency treatment prior to mounting. The apoptotic cell stainingmethod can identify apoptotic cells at different phases and displaying cell apoptosis statuses comprehensively, and is simple to operate, low in staining cost and suitable for popularization and application.

Description

Technical field [0001] The invention belongs to the field of cell identification, and specifically relates to a staining method for apoptotic cells. Background technique [0002] Apoptosis is the death of a single cell or a small group of cells in a living body. The plasma membrane of the dead cell is not broken, it does not cause the autolysis of the dead cell, and it does not cause an acute inflammatory reaction. The occurrence of apoptosis is related to gene regulation, and it is also called programmed cell death. Apoptosis is a basic physiological mechanism of the body, and it runs through the entire life activities of the body, including the development of embryos, the alternation of old and new cells, the normal degeneration and atrophy of certain tissues, or the spontaneous inhibition of tumor cells. [0003] At present, the conventional in situ identification technology of apoptotic cells is mainly a staining method in addition to observation by electron microscope. [0004...

AI Technical Summary

Problems solved by technology

TUNEL in situ hybridization staining is a commonly used staining method for apoptotic cells. However, this method has the following disadvantages: the tissue section needs to be digested during the staining process, which causes a certain degree of damage to the tissue structure of the section; it can only mark early apoptosis Cells: Reagents are expensive, the staining process requires high requirements, it is not easy to operate successfully, and false negative results often appear

Images