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Coccid slide specimen manufacturing method

A technology for making glass slides and specimens of scale insects, which is applied in the field of biological specimen making, can solve the problems of difficult coloring of specimens, low success rate of preparation, and great toxicity to human body, and achieves the effects of clear observation and identification, high quality and clear specimens.

Inactive Publication Date: 2018-10-16
河南林业职业学院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the traditional method of preparing scale insect slide specimens, there are problems that the specimens are not easy to be colored and the success rate of making slides is low, and xylene is often used as a transparent agent. Xylene has been included in the list of three types of carcinogens by the International Agency for Research on Cancer of the World Health Organization Among them, it is more harmful to the human body, unsafe, and not environmentally friendly.

Method used

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  • Coccid slide specimen manufacturing method

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Effect test

Embodiment 1

[0021] A method for preparing a scale insect slide specimen, comprising the following steps:

[0022] S1, collecting live scale insects and storing them at -10°C to obtain untreated scale insects;

[0023] In S2 and S1, the scale insect to be treated made a hole in the epidermis of the scale insect, then soaked in NaOH solution with a mass concentration of 5% for 1 hour, and squeezed to dissolve the internal substances of the scale insect body, and retained the scale insect shell to obtain Soften scale insects;

[0024] If the scale insects to be treated in S2 belong to wax scales with a higher degree of ossification, put them in a metal bath at 45°C for 0.5h before soaking;

[0025] S3, wash the softened scale insects in S2 with distilled water-acetic acid solution, the washing time is ≥ 1 hour, and then soak them in acid fuchsin solution for 2 hours to obtain dyed scale insects;

[0026] Wherein, the distilled water-acetic acid solution is prepared according to the followi...

Embodiment 2

[0033] A method for preparing a scale insect slide specimen, comprising the following steps:

[0034] S1, collecting live scale insects and storing them at -5°C to obtain untreated scale insects;

[0035] In S2 and S1, scale insects to be treated pierce 2 holes in the skin of the scale insects, then soak in NaOH solution with a mass concentration of 8% for 6 hours, and squeeze the internal substances of the scale insects to dissolve, retain the scale insect shell, and obtain softening scale insects;

[0036] If the scale insect to be treated in S2 belongs to the scale insect with a higher degree of ossification, put it in a metal bath at 50°C for 1 hour before soaking;

[0037] S3, distilled - acetic acid solution water to wash the softened scale insects in S2, the washing time ≥ 1h, and then put into the acid fuchsin solution for 24h to obtain the dyed scale insects;

[0038] Wherein, the distilled water-acetic acid solution is prepared according to the following method: 2 ...

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Abstract

The invention belongs to the technical field of biological specimen manufacturing and in particular relates to a coccid slide specimen manufacturing method which comprises the following steps: collecting live coccid, carrying out low-temperature treating, and soaking into a 5-8% NaOH solution to carry out softening treatment; flushing, dyeing with an acid fuchsin solution, soaking with 50%, 70% and 100% alcohol solutions in sequence, finally soaking in clove oil, and drying specimens. The method provided by the invention is simple, convenient and safe, and a coccid slide specimen is clear, complete, uniform in coloring and high in quality.

Description

technical field [0001] The invention belongs to the technical field of biological specimen preparation, and in particular relates to a method for preparing a scale insect slide specimen. Background technique [0002] Scale insects belong to the order Hemiptera and the superfamily Scaloidaceae. According to the statistical report of scale insect net, there are more than 8,000 species of scale insects recorded in the world at present; there are 1,155 species of scale insects in 14 families, 302 genera and 1,155 species recorded in my country. Scale insects are mostly small in size and live in hiding. They are often covered with shells or waxy secretions for protection. Their vitality and fecundity are extremely strong, and they are prone to outbreaks. Most species of scale insects are important agricultural and forestry pests. They often gather on the surface of plants and suck juice to cause damage, causing branches and leaves to wither, growth potential to decline, and even...

Claims

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Application Information

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IPC IPC(8): G01N1/30
CPCG01N1/2813G01N1/30G01N2001/302
Inventor 孙丹萍刘玉卿王朝阳
Owner 河南林业职业学院
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