Capsule staining method for streptococcus pneumoniae
A Streptococcus pneumoniae and capsule staining technology, which is applied in the field of Streptococcus pneumoniae capsule staining, can solve the problems of high capsule shape requirements, high operation skills requirements, and high water content of bacterial capsules, and achieve high-efficiency strains The effect of low identification, skill and proficiency, and simple methods
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[0051] Embodiment 1, the effect identification of Streptococcus pneumoniae capsule staining method
[0052] The present invention selects 7 serotype strains (table 1) in the 23-valent pneumococcal polysaccharide vaccine to carry out the effect display of the Streptococcus pneumoniae capsule staining method, and the steps are as follows:
[0053] 1. Preparation of bacterial specimens:
[0054] Take the lyophilized strain of Streptococcus pneumoniae, add 200 μl of sterile saline to dissolve, and repeatedly blow and beat to mix. Take 100 μl on 10% sheep blood MH medium, and spread evenly with a glass triangular applicator. placed in 6% CO 2 In the incubator, cultured upside down at 37°C for 16h-18h, washed with physiological saline, and smeared for later use.
[0055] 2. Smear: Take a small amount of prepared bacterial specimen and apply it evenly on a clean glass slide, scrape it into a thin layer, and let it dry naturally (because the water content of the capsule is above 90...
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