47results about How to "Long breeding cycle" patented technology

The present invention belongs to the field of rice heterotic vigor utilizing technology. Based on the genetic feature that rice glutinous albumen of rice is controlled by the single recessive gene wx, mutagenesis method is adopted in treating hybrid rice parents to obtain corresponding glutinous property maintaining line and restoring line, the glutinous property maintaining line is made to breed and back-cross with original hybrid combination to breed the corresponding glutinous sterile line, and glutinous rice hybrid combination is further compounded and new commercial glutinous rice variety is cultured. Thus bred hybrid glutinous rice variety has the yield vigor and other excellent characters of original variety maintained, and has the advantages of high breeding efficiency, short period and low cost.

The invention relates to a rapid cultivating method for a high-yield and high-quality multiple resistant rice restorer. The method comprises the steps of selecting eight rice species having the characters of high yield, high quality, insect resistance and disease resistance as parents, hybridizing every two parents with different target characters to obtain four first-filial generations F1; hybridizing every two of the four F1 to obtain two composite cross generations F1; performing molecular marker-assisted selection on the two composite cross generations F1 single plants in a seedling stage, screening two composite cross generations F1 integrating target genes, carrying out matched hybrid to obtain multiple composite cross generations F1; performing molecular marker-assisted selection on the multiple composite cross generations F1 single plants in a seedling stage to obtain a single plant integrating all target genes, carrying out anther culture to obtain H0 generation anther-culture lines, screening diploid lines; performing molecular marker-assisted selection, agronomic characters, quality and resistance screening on H1 generations to obtain a target high-yield and high-quality multiple resistant rice restorer, carrying out combined test with a series of two-line sterile lines, and screening out advantageous combination.

The invention particularly relates to a method for breeding chickens under a forest and belongs to the technical field of poultry breeding. The breeding cycle of the free range chickens under the forest is long, the breeding cycle of cocks is not shorter than eight months, the breeding cycle of hens is not shorter than one year, mixed feed is not used, and only corn and rice serve as auxiliary feed; due to natural stocking, eggs are high in nutritive value, and edible chicken contains no growth hormones and is excellent in taste; scientific management and gradual adaptive stocking are carried out, and the survival rate of a chick flock is greatly increased; stocking starts to be carried out on the chickens in the breeding period, the meat of the chickens stocked in the forest is delicious, and the meat quality of the chickens is greatly improved; the chickens stocked in the forest can eat pests and grass, great benefit is achieved on preventing insect pests of the forest, chicken manure can increase fertilizer for the forest, and the breeding cost of the chickens is reduced.

The present invention relates to saline algae, provides one kind of low temperature tolerant saline algae and its ultraviolet mutagenesis breeding process and identifying method. The low temperature tolerant saline algae features its light illuminating growth at 3-10deg.c to reach (1.05-8.11)x10<6> cell / ml in the cell density 2.1-21 times higher than the contrast; the genetic similarity coefficient to wild strain of 0.680-0.910; and increased or decreased protein zone(s) compared with wild strain.

The invention discloses a breeding method of solkworn strain wherein the lavae is fluoride-resistant and the imago fin has no palea scale. The breeding method uses the Chinese race 'Jiang Song' and the Japanese race 'Hao Yue' as recurrent parents and the T6 is used as the fluoride-resistant high-grade object character donor parent and the gene mutation strain U13 is used as the no palea scale gene donor. The first hybridization generation obtained by hybridizing the recurrent parents with theU13 is hybridized with the T6 and then the obtained filial generation is continuously backcrossed with the recurrent parents and each generation is subjected to individual selection during breeding and the selected individual is marked by molecule to select the genotype thereof two silkworn strains 'JsFnlw' and 'HyFnlw' are selected after self-cross homozygosis for six generations, wherein the lavae is fluoride-resistant and the imago fin has no palea scale. The 'JsFnlw' and 'HyFnlw' have strong physical constitution and the lavae whole-instars fluoride-resistance rate is above 100mg / kg and the fluoride-resistance performance is higher than the Chinese race 'Jiang Song' and the Japanese race 'Hao Yue' by 50% and the imago fin has no palea scale, in addition the micro-dust produced during seed-production is reduced by 50% than the common silkworn strain and the practical character corresponds with the utility breed level.

The invention belongs to the field of molecular breeding, and relates to a molecular breeding method for breeding a new anti-verticillium cotton seed. The method comprises the following steps: taking a chromosome segment introgression line 1L095 of sea-island cotton of which the collection number is CGMCC No.5574 as a female parent and a chromosome segment introgression line 1L154 of sea-island cotton of which the collection number is CGMCC No.5575 as a male parent to crossbreed a generation I seed, and obtaining F2 from the crossbred generation I seed, wherein each generation is collected bya single strain; and selecting by taking a molecular marker for assistance to obtain a novel multi-strain prevention cotton strain. Through the adoption of the method, the novel multi-strain prevention cotton strain-Nannong haidao V is bred. As the molecular marker is taken for assistance to select a target trait, the novel multi-strain prevention cotton strain can be bred in 2 to 3 years fast and effectively. The corresponding disease index of Nannong haidao V is 15.51 to 20.97 percent.

The invention provides an indoor culturing method for crayfish. Biological habits of the crayfish are fully known, deep and shallow culture water levels are designed in every indoor culture box, shelters are made, small bricks are placed, water changing and addition of floating plants and animals are carried out regularly, and the habitat of crayfish under natural conditions are created to make the crayfish live in an environment close to the natural state, so the survival rate of the crayfish is high, the feed utilization rate is improved, the growth rate is increased, the mutual killing ratein the eclosion period is reduced, and the quality and the yield are improved. The method belongs to the field of crayfish culturing.

The ultraviolet ray mutagenesis, selective breeding and identification method of cold tolerant Dunaliella includes: inoculating Dunaliella to culture medium; lighting and dark inducing for synchronized growth; mixing Dunaliella liquid with iodine solution or bromophenol blue solution to deactivate Dunaliella, injecting Dunaliella liquid to culture dish, mutagenesis under ultraviolet lamp before dark culturing, mixing with fresh culture liquid and painting the mixture to Dunaliella culture medium for low temperature lighting culture; inoculating single Dunaliella colony to the culture liquid and low temperature lighting culturing; sampling detection to comparing the low temperature lighting growth curves of cold tolerant Dunaliella mutant and wild prototype strain; extracting total DNA for RAPD comparison; calculating genetic similarity coefficient; extracting total protein and post-electrophoresis staining while record results; comparing protein electropherogram; and confirming the obtained cold tolerant Dunaliella mutant.

The invention relates to a precise combined feeding method for regulating water quality in aquaculture, and belongs to the technical field of the aquaculture. The precise combined feeding method for regulating the water quality in the aquaculture includes steps: using high and low protein feed in mixing mode during the whole aquaculture process, regulating a carbon-nitrogen ratio in the medium period of the aquaculture, and regulating the water quality in the later period of the aquaculture. By using the precise combined feeding method for regulating the water quality in the aquaculture, the water quality can be regulated well, bacilli and lactic acid bacteria in a water body become dominant bacteria, fishes can grow well and quickly by throwing the feed in the later period of the aquaculture, a culture cycle is shortened, yields and efficiency are increased, and a feed coefficient is reduced.

The invention discloses a propagation method of dendrobe seeds, which belongs to the technical field of artificial cultivation of dendrobe. The method comprises the following steps of: seeding in a seeding medium, performing propagation cultivation in a propagation medium, performing rooting cultivation in a rooting medium, and performing strong seedling cultivation in a strong seedling medium, so as to obtain cultivation seedlings. According to the propagation method, the technical problems of small base of explants, difficulty in disinfection, slowness in differentiation of original corm, long propagation cycle, low propagation multiple, difficulty in establishment of an asepsis system and the like of the prior art are effectively solved.

The invention discloses a cultivation method of high-temperature-resistant gelidium amansii and belongs to the technical field of gelidium amansii study and development application. High-temperature-resistant gelidium amansii can be obtained by accurate screening through gradient high-temperature stress, low-temperature culture and high-temperature screening after mutagenization, and the method ishigh in repeatability, reasonable and easy to implement and convenient and quick in application. Gelidium amansii obtained by cultivation can normally grow at 32 DEG C and is high in sea area breeding growth speed. High-temperature-resistant gelidium amansii mutant obtained by induced cultivation enables gelidium amansii breeding enterprises to realize gelidium amansii summer normal breeding without changing a breeding mode by the gelidium amansii breeding enterprises, breeding period is prolonged, year-round production bearing can be realized by the gelidium amansii enterprises, and economicbenefit of gelidium amansii industry is increased greatly.

The invention discloses a new soft rice waxy gene wx-C39 functional marker and application thereof, and belongs to the field of agricultural biological engineering. A molecular marker primer is WxC39-F:CGTCCCAGCTCGCCACCT and WxC39-R:CCACGCTGCACCGACCG, and if an amplification product is 173bp, it is indicated that wx-C39 homozygous genotype is carried, wherein the amylose content is about 5%; if 184bp is obtained through amplification, it is indicated that a homozygous genotype of Waxy is carried, wherein the amylose content is about 15%; if two bands are amplified simultaneously, it is indicated that a heterozygous genotype exists, wherein the amylose content is about 15%. The marker and a method can be used for filial generation genotype detection, and the soft rice breeding process can be accelerated conveniently. The method is high in accuracy, easy to operate, high in detection efficiency and low in cost, and has very high practical value.

The invention discloses a functional marker for detecting a broad-spectrum rice blast resistance gene PigmR and an application thereof. The marker includes a primer pair GMR-3 and / or prime pair Actin1-1, GMR-3 is designed according to the functional sequence of PigmR gene and completely co-separated with PigmR gene. The sample which could amplify PigmR gene contained 98 bp of PigmR gene and couldnot amplify the PigmR gene, which indicated that PigmR gene was not carried. Actin1-1. False negative results caused by the failure of PCR amplification of PigmR gene product in the process of excluding samples and experimental operation, the amplification product size is 146 bp. The marker and the method disclosed by the invention can be used for gene detection of variety resources and hybrid progeny, which is favorable for speeding up the breeding process of rice blast resistance. The method has the advantages of high accuracy, simple operation, high detection efficiency and low cost, and has strong practical value.

The invention relates to a cultivation method of a rhopilema esculentum offspring seed. The cultivation method comprises the following steps: according to a model for gradually cultivating a rhopilema esculentum seedling, taking the stage from scyphistoma to butterfly-like body as a temporary first-class rhopilema esculentum culturing seedling, taking the rhopilema esculentum offspring seed with the shed diameter from 1-1.5cm to 3-5cm as a temporary second-class rhopilema esculentum culturing seedling, and taking the rhopilema esculentum with the shed diameter from 3-5cm to 10-20cm as a temporary third-class rhopilema esculentum culturing seedling. The cultivation method is characterized in that the temporary first-class rhopilema esculentum culturing seedling can provide the offspring seed to the temporary second-class rhopilema esculentum culturing seedling, the temporary second-class rhopilema esculentum culturing seedling can provide the offspring seed to the formed rhopilema esculentum culturing pond and the temporary third-class rhopilema esculentum culturing seedling, when the survival rate of the temporary second-class rhopilema esculentum culturing seedling in the formed rhopilema esculentum culturing pond is low, the temporary third-class rhopilema esculentum culturing seedling can replenish the offspring seed, and can provide the large-scale rhopilema esculentum seedling to the rhopilema esculentum forming culturing pond in the middle of August. In the cultivation method, according to the model for gradually cultivating the rhopilema esculentum seedling, the culturing cycle of the formed rhopilema esculentum is prolonged, multiple seedlings can be cultivated in one year, and the economic benefit of the rhopilema esculentum is improved in cultivation.

The invention relates to a cultivation method of a rhopilema esculentum offspring seed. The cultivation method comprises the following steps: according to a model for gradually cultivating a rhopilema esculentum seedling, taking the stage from scyphistoma to butterfly-like body as a temporary first-class rhopilema esculentum culturing seedling, taking the rhopilema esculentum offspring seed with the shed diameter from 1-1.5cm to 3-5cm as a temporary second-class rhopilema esculentum culturing seedling, and taking the rhopilema esculentum with the shed diameter from 3-5cm to 10-20cm as a temporary third-class rhopilema esculentum culturing seedling. The cultivation method is characterized in that the temporary first-class rhopilema esculentum culturing seedling can provide the offspring seed to the temporary second-class rhopilema esculentum culturing seedling, the temporary second-class rhopilema esculentum culturing seedling can provide the offspring seed to the formed rhopilema esculentum culturing pond and the temporary third-class rhopilema esculentum culturing seedling, when the survival rate of the temporary second-class rhopilema esculentum culturing seedling in the formed rhopilema esculentum culturing pond is low, the temporary third-class rhopilema esculentum culturing seedling can replenish the offspring seed, and can provide the large-scale rhopilema esculentum seedling to the rhopilema esculentum forming culturing pond in the middle of August. In the cultivation method, according to the model for gradually cultivating the rhopilema esculentum seedling, the culturing cycle of the formed rhopilema esculentum is prolonged, multiple seedlings can be cultivated in one year, and the economic benefit of the rhopilema esculentum is improved in cultivation.

The invention discloses an additive and application thereof. The additive is prepared from the following components in parts by mass: 8 to 12 parts of glucurolactone and 3 to 6 parts of an acidifying agent, and the acidifying agent is prepared from the following components in parts by mass: 1.2 to 2.4 parts of formic acid, 1.2 to 2.4 parts of ammonium formate, 0.18 to 0.42 part of acetic acid, 0.18 to 0.42 part of propionic acid and 0.18 to 0.42 part of sorbic acid. The exogenous glucurolactone is used as an additive and is matched with the acidifying agent, the glucurolactone is mainly used for relieving the fatty liver, and the acidifying agent is used, so that the stability of the glucurolactone can be improved, and the content of active ingredients which play a role of the glucurolactone can be increased; and the acidifying agent can effectively reduce harmful bacteria in the feed, kill the harmful bacteria in intestinal tracts, reduce toxicity of the harmful bacteria and increase the content of beneficial bacteria in the intestinal tracts when being added to the feed for the laying hens, and by combined action of the glucurolactone and the acidifying agent, the fatty liver syndrome of the laying hens is effectively prevented and treated, and the production performance of the laying hens in the later egg laying period is improved, so that the breeding cycle of the laying hens is prolonged.

The invention belongs to the field of molecular breeding, and relates to a molecular breeding method for synchronously improving the length, strength, and fineness of cotton fiber through polymerizing chromosome segment introgression lines. The method comprises the following steps: taking two introgression lines, namely IL080-D6-1 and 1047 (IL019-A2-6 chromosome segment introgression line), as the non-recurrent parents, respectively subjecting the non-recurrent parents to carry out hybridization with a recurrent parent (Xinjiang 0768) for one time, backcrossing two generations, in BC2F1 making strains containing different chromosome segments intercross with each other so as to obtain double-segment integrated F2 segregation population, identifying the double-segment homozygous strains through each two molecular labels on two segments, carrying out field tests under four circumstances, and keeping on selfing for two generations so as to obtain a double-segment homozygous novel cotton breed, whose fiber length, fiber strength and fineness have been prominently improved. A high-quality and high-yield novel cotton species, named "Nannong 08-66", has been cultured by utilizing the method provided by the invention.

The invention belongs a breeding technology of shellfishes and particularly relates to a breeding method of cultivating hybridized scallops of purple scallops and bay scallops in the south and raisingthe hybridized scallops in the north in order to achieve large-scale cultivation of the scallops. According to the breeding method, the young hybridized scallops of the purple scallops and bay scallops are cultivated in the south during autumn and then transported to northern sea areas for follow-up raising in next spring. A new hybridized species selected from the first generation of the hybridized scallops of the purple scallops and bay scallops for breeding is adopted as the breeding scallops, the young scallops are cultivated in the south during autumn and then transported to the northernsea areas for follow-up raising, and therefore the commercial scallops with large specification are cultivated. Through establishment of a breeding pattern, the breeding time of the hybridized scallops of the purple scallops and bay scallops is prolonged, the breeding range is expanded, the specification of the bred scallops can be effectively increased, the value of scallop products is higher, and the quality and safety of the scallop products are effectively ensured. The yield of high-quality marine protein is increased, the income of fishermen is increased, and a new way to healthy sustainable development of the scallop breeding industry is created.

The invention discloses a method for breeding meloidogyne hapla by using eupatorium adenophora Spreng., and belongs to the technical field of biology. The method comprises the steps of collecting or purchasing the meloidogyne hapla or oocysts for cold storage for use, preparing a culture material of the eupatorium adenophora Spreng., preparing a planting medium, and conducting inoculation. The method has the advantages that the eupatorium adenophora Spreng. is high in vitality and adaptability, capable of surviving easily, easy to plant, insensitive to most kinds of meloidogyne spp. and high in specific correspondence to the meloidogyne hapla, and the single meloidogyne hapla is easily obtained; the cost of breeding the meloidogyne spp. by the eupatorium adenophora Spreng. is low; compared with other crops, the breeding cycle is longer, and living bodies of the meloidogyne hapla can be continuously obtained; repeated use can be achieved after one-time inoculation.

The invention discloses a three-step cultivation method for improving the seedling emergence rate and early fruit bearing of cherry hybrids. The method comprises following steps:1) hybrid seed collecting; 2) hybrid seed disinfecting and storing; 3) accelerating germination of hybrid seed; 4) sowing and seeding raising for hybrid seeds; 5) transplanting for hybrid seeds; 6) field hybrid seedling root-irrigation, spraying foliar fertilizer and growth regulator; 7) hybrid seed field planting and cutting, controlling vertical growth and promoting new branches; 8) pinching and promoting flower buddifferentiation. The invention accelerates the growth of plants by artificially breaking dormancy, seedlings of the hybrid seeds in the same year and spraying plant growth regulators and insecticides,fungicides and foliar fertilizers; planting hybrid seedlings and cutting, controlling erect growth, and promoting new shoots; pinching, promoting flower bud differentiation, achieving advancing of the first flowering time of hybrid seedlings, and shortening the cross-breeding cycle of Chinese cherry.

The invention belongs to the field of red alga culture and particularly relates to an asparagus and kappaphycus alvarezii sinking-rope raft culture device and an assembling method. The culture device comprises seedling ropes, a cover net and a matched processing device. The processing device includes a hard horn-shaped open section and a hard cylindrical section with two open ends. A cover net protection part is added, achievement is promoted through a matched processing facility, asparagus and kappaphycus alvarezii plant loss in the culture process can be effectively reduced, an asparagus culture period can be prolonged, and culture benefits can be increased.

The invention discloses a functional marker AS-ALS for anti-imazethapyr herbicide genotype detection and an application of the functional marker AS-ALS. The marker comprises a primer combination F1N (AS-ALS-F1 and AS-ALSn-R2-2) and a primer combination F1M (AS-ALS-F1 and AS-ALSm-R2-1), the F1N can amplify a stripe sample, namely, an ALS-G genotype, the F1M can amplify a stripe sample, namely, an ALS-A genotype, both the F1N and the F1M can amplify a stripe sample, and namely, a heterozygous genotype of the ALS-G and the ALS-A. The marker and a method can be used for genotype detection of variety resources and filial generation and are beneficial to accelerating an herbicide-resistant breeding progress. The method is high in accuracy and detection efficiency, simple to operate and low in cost and has a high practical value.

The invention provides a pond culture system and method for controlling continuous emergence of shrimp seeds in spring, summer and autumn for procambarus clarkii Girard. The pond culture system comprises a pond main body, a water intake and drainage device, an oxygenation device and an anti-escape device. The method comprises the following steps of 1) constructing the pond culture system; 2) preparing a pond; 3) throwing in parent shrimps and carrying out management; (4) carrying out management in spring; and 5) carrying out management of emergence of the shrimp seeds in summer and autumn. According to the method, through an ecological regulation and control method, the shrimp seeds of the procambarus clarkii Girard come out from caves after June, so that the requirement for the shrimp seeds of procambarus clarkii Girard culture in the next half year can be met, and therefore the breeding period of the procambarus clarkii Girard is prolonged, and the method is high in operability and has a relatively large popularization value.

The invention relates to molecular markers for identifying early- and late-maturing characteristics of double-cropping Zizania latifolia and application and an obtainment method thereof, which belongto the technical field of molecular biology. The molecular markers for identifying early- and late-maturing characteristics of double-cropping Zizania latifolia are molecular markers UeS1, UeS2, UeS3,UeS4, UeS5, UeS6, UeS7 and UeS8. According to the invention, by screening early- or extremely late-maturing varieties by means of the SNP (Single Nucleotide Polymorphism) markers, an excellent double-cropping Zizania latifolia variety can be selected in advance, the workload is reduced, and the efficiency of screening is increased.

The invention discloses a native chicken breeding method which includes the steps: (1) building and managing native chicken breeding rooms; (2) managing feeding and disease prevention at stages afternative chickens enter the breeding rooms; (3) replacing the breeding rooms of the native chickens in a way leading manner of food, and increasing the breeding rooms to perform normal feed management.The method has the advantages that native chicken breeding cycle is long, various feed is mixed to feed the native chickens, the native chickens are naturally stocked, so that nutritive values of eggsare high, edible chicken does not contain any growth hormone, is better in taste, baby chickens are scientifically bred, growth is scientifically managed, the survival rate of the baby chickens is greatly increased, chickens in the replacement period are stocked in a forest, so that chicken is delicious and greatly improved, the chickens stocked in the forest can eat bugs and grass, pest controlof the forest is effectively achieved, chicken manure increases fertilizers for the forest, chicken breeding cost is reduced, vaccines are injected every month, and diseases of the chickens are avoided.

The invention belongs to the technical field of aquaculture, and particularly relates to a special feed for ictalurus punetaus and a high-yield and high-efficiency culture model thereof. The special feed for the ictalurus punetaus has the advantages that the ictalurus punetaus can digest and absorb the feed well; the growth speed of the ictalurus punetaus is increased; the feed coefficient is reduced; the stability of the feed in water is high; aquaculture water quality is not prone to pollute; feed nutrition is balanced; a liver-protecting and gallbladder-protecting additive is added, so that the health of the ictalurus punetaus is protected, the survival rate and digestibility are increased, and the growth speed is high; and after eating the feed, the ictalurus punetaus has a stable body color and has no yellow-flesh risk; and the high-yield and high-efficiency culture model has the advantages that diseases are reduced; diseases can be effectively controlled through low-density culture; the death rate can be reduced; with the use of animal protection products such as a substrate improver and a disinfection product, the diseases are reduced; two batches of ictalurus punetaus are harvested in one year, the yield and efficiency are increased, the culture period is shortened, and the fund pressure of a culturist is relieved.

The invention discloses a method for cultivating rice field eels in circulating water of net cages in soil-based greenhouses, which comprises the following steps: 1) selecting a breeding area; 2) building a breeding greenhouse and a water storage pool; 3) erecting net cages. The invention realizes the control of the rice field eel breeding conditions through the sunshade heat preservation shed, the net cage, the breeding shed and the water storage pool, and then achieves the purpose of stably increasing production. Compared with pure pond cage culture, the present invention has the advantages of controllable culture conditions, long culture cycle, less disease and easy management, and does not require huge initial investment like industrial culture of rice field eel, and is a low-cost and high-yield rice field eel Breeding facilities have the advantages of increasing eel breeding cycle, high and stable output, less disease and easy management.