Method for rapidly and specifically detecting curdlan content

A curdlan, specific technology, applied in the measurement of color/spectral characteristics, weighing by removing certain components, etc., can solve the problems that cannot be quantitatively analyzed with curdlan, so as to promote the level of fermentation control and solve the process cumbersome effect

Inactive Publication Date: 2017-09-05
GUANGXI UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

[0004] Studies have shown that β-1,3-D-glucan can be exclusively stained by aniline blue, which is applied to staining and identification of curdlan producing bacteria by adding aniline blue to solid medium, but because aniline blue is only A stable blue color appears only under neutral and acidic conditions, while curdlan can only be dissolved under alkaline conditions, which makes the aniline blue staining method only applicable to the cultivation of curdlan-producing bacteria on solid plates for a long time Based on the primary screening and cannot be used for quantitative analysis of curdlan

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  • Method for rapidly and specifically detecting curdlan content
  • Method for rapidly and specifically detecting curdlan content

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Embodiment 1

[0026] A method for rapid and specific detection of curdlan content, using commercially available curdlan as a standard product to prepare a standard curve, comprising the following steps:

[0027] (1) Preparation of aniline blue solution: Accurately weigh 0.5 g of aniline blue solid, add deionized water and stir, after fully dissolving, use a volumetric flask to set the volume to 100 mL, and prepare a 0.5% water-soluble aniline blue solution;

[0028] (2) Preparation of curdlan standard alkali solution: Dry the commercially available curdlan standard product to constant weight, accurately weigh 0.25g in a beaker, add 60mL 0.1mol / L NaOH solution and magnetically stir until the polysaccharide is completely dissolved, then Dilute to 100mL with 0.1mol / L NaOH solution to obtain 0.25% curdlan standard alkali solution;

[0029] (3) Preparation of curdlan standard curve: Take a series of curdlan standard alkali solutions of different volumes with 0.25% curdlan standard alkali solutio...

Embodiment 2

[0032] A method for rapid and specific detection of curdlan content, extracting pure curdlan from fermentation broth to prepare a standard curve and applying it to colorimetric determination of curdlan content in fermentation broth, comprising the following steps:

[0033] (1) Preparation of curdlan fermentation broth: Taking the strain Agrobacterium radiobacter A-15 preserved in the College of Life Science and Technology of Guangxi University as an example, transfer from the inclined plane to seed culture based on 32°C, 220r / min shaker culture for 18h, and then 12% of the inoculum size was transferred to the fermentation medium, and cultured on a shaking table at 32°C and 240r / min for 120h to obtain curdlan fermentation liquid;

[0034] Described seed culture medium is 0.3% (NH 4 ) 2 HPO 4 , 2% Dextrose Monohydrate, 0.15% KH 2 PO 4 , 0.1% MgSO 4 , 0.1% corn steep liquor, pH7.2;

[0035] Described fermentation medium is 5% glucose, 0.03% (NH 4 ) 2 HPO 4 , 0.2% KH 2 P...

Embodiment 3

[0049] A method for rapid and specific detection of curdlan content, the specific detection of curdlan comprises the following steps:

[0050] (1) Preparation of aniline blue solution: Accurately weigh 0.5 g of aniline blue solid, add deionized water and stir, after fully dissolving, use a volumetric flask to set the volume to 100 mL, and prepare a 0.5% water-soluble aniline blue solution;

[0051] (2) Preparation of curdlan standard alkali solution: Dry the commercially available curdlan standard product to constant weight, accurately weigh 0.25g in a beaker, add 60mL 0.1mol / L NaOH solution and magnetically stir until the polysaccharide is completely dissolved, then Dilute to 100mL with 0.1mol / L NaOH solution to obtain 0.25% curdlan standard alkali solution;

[0052] (3) Preparation of standard alkaline solution of other types of polysaccharides: Dry the commercially available soluble starch, sodium carboxymethyl cellulose, dextran standard and the levan sample made by Guangx...

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Abstract

The invention belongs to the technical field of food and fermented product detection, and particularly relates to a method for rapidly and specifically detecting curdlan content, and an application of the method. The method for rapidly and specifically detecting the curdlan content includes the steps: preparing aniline blue solution; preparing curdlan standard alkali solution; making a curdlan standard curve; measuring the content of a curdlan sample. According to the method, the content of curdlan in various solution such as fermented solution can be rapidly detected, change of the yield of the curdlan in the fermentation process can be rapidly tracked, fermentation production strategies are correspondingly adjusted, and the production capacity of different strains in the liquid fermentation process is rapidly compared, so that curdlan strain breeding and fermentation control level is promoted.

Description

technical field [0001] The invention belongs to the technical field of detection of food and fermented products, and in particular relates to a method for rapid and specific detection of curdlan content and an application thereof. Background technique [0002] Curdlan is a glucan polymerized by β-1,3-D glycosidic bonds, which can be fermented by microorganisms using carbon sources such as glucose and sucrose. Curdlan is widely used in food, medicine, feed, health care and other fields, and is a high value-added product. [0003] At present, the content of curdlan is mainly determined by phenol-sulfuric acid colorimetric method, Congo red colorimetric method and extraction weighing method. Among them, the principle of the phenol-sulfuric acid colorimetric method for the determination of polysaccharides is to first hydrolyze the polysaccharides into monosaccharides such as glucose under the action of concentrated acid, and then quickly generate furfural derivatives and then u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N5/04G01N21/31
CPCG01N5/04G01N21/31
Inventor 杨辉李文强朱萍梁海秋周河治
Owner GUANGXI UNIV
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