48 results about "Callose" patented technology

The invention relates to an optionally foamed sealing composition for preformed seals which can swell when treated with water. The invention also relates to a method for the production thereof out of natural rubber and / or elastomers with a matrix comprised of natural rubber / elastomer components and particle-shaped water absorbing material stored therein. The water absorbing material is a combination of (A) polysaccharide(s) selected from cellulose, starch, starch derivatives removed from grafted starch, amylose, amylopectin, dextran, pectin, inulin, chitin, xanthan, alginic acid, alginates, carrageenan, pustulan, callose, laminarin, guluronic acid, pullulan, lichenin or mixtures of the same with (B) a highly water absorbent synthetic polymer selected from polymers based on (meth)acrylate, poly(meth)acrylic acid and the salts thereof, polyacrylamide, polyalcohols or copolymers of said synthetic polymers. The invention also relates to additional cross-linking and processing auxiliary agents and to property improving agents. It is possible to securely seal superstructures, substructures, tunnels and canals with the assistance of the inventive sealing compositions.

The invention belongs to the field of agricultural biotechnology, and specifically relates to a gene GhABC related to resistance to cotton verticillium wilt, and encoding protein and applications thereof. The nucleotide sequence of the gene GhABC related to resistance to cotton verticillium wilt is as shown in SEQ ID No.2, and the amino acid sequence of the encoding protein is as shown in SEQ ID No.1. After a gene silencing plant is inoculated with a spore suspension of cotton verticillium wilt pathogen Vd080, the silencing plant is more susceptible to disease. It is, by the mechanism research, found that when the synthesis of xylem and tannin in the silencing plant decreases, the ROS decreases and the expression of some defense genes is regulated down. The studies demonstrate that the GhABC is positively correlated with the resistance to cotton verticillium wilt, and the GhABC can be used as a candidate gene for cotton disease-resistance breeding.

The invention provides a method for quickly identifying cross-compatibility of rose hybrida. The method is characterized by comprising the following steps of: collecting and storing pollen, performing castration, performing artificial pollination, cutting a sample to be identified, fixing the cut sample in FAA stationary liquid, transferring the cut sample to a 70 percent ethanol solution after 24 hours, taking out for washing, placing in an 8mol / L NaOH solution, performing water bath at the temperature of 55 to 60 DEGC for 15 to 20 minutes, and then standing at the temperature of 20 to 28 DEGC for 3 to 3.5 hours, soaking into water for at least 1 hour, transferring to a 0.1 percent dye solution of aniline blue, dyeing for 2 to 3 hours, observing stigma callose deposition, pollen germination and growth of pollen tubes by using a fluorescence microscope to identify the cross-compatibility of rose hybrida. By the method, the cross-compatibility of rose hybrida can be identified within 30 to 48 hours; and moreover, the method is simple and easy to operate and can effectively reduce the waste of breeding resources and improve breeding efficiency.

A leaf sheath tissue section manufacturing method for enabling users to observe callose of rice lead sheath tissues belongs to the technical field of micro-structural slicing and includes: (1) preprocessing of materials; (2) sample fixing; (3) section freezing; (4) section unfolding; (5) dying; and (6) microscopic observation and photographing. The leaf sheath tissue section manufacturing method remarkably shortens time for sample fixing, slicing and dying, basically the whole process from preprocessing of samples to observation and photographing under a fluorescence microscope can be finished in about 12 hours, the section obtained through the lead sheath tissue section manufacturing method is complete in tissue structure, the images are clear, and simultaneously the leaf sheath tissue section manufacturing method effectively solves the problem that slicing with hands can lead the tissues of the materials to be fragile, thickness of the sections to be uneven, a paraffin slicing process is complex, the period is long and the like. Working efficiency is remarkably improved.

The present invention refers to a capsule (1) enclosing an agrochemical (7), a method for producing the said capsule (1), a packing method of any biological form of a vegetative source (4), such as seed and / or embryo and / or spore and / or callose and / or meristem and / or a combination of these of at least one culture, for the use of a capsule (1) enclosing any biological material of a vegetative source (4) and a cultivation method of a culture employing the said capsules (1).

The invention discloses the application of DCM1 protein and coding gene thereof in regulating male fertility of plants. The invention provides a method for cultivating a male sterile plant, comprisingthe following steps: inhibiting the expression of DCM1 gene in a target plant to obtain a male sterile plant; DCM1 gene encode DCM1 protein. The DCM1 protein is a protein composed of an amino acid sequence shown in SEQ ID NO: 1 in the sequence table. The inventors of the invention, through CRISPR-Knockout of Cas9 gene in wild-type rice can cause pollen abortion and produce male sterile lines. DCM1 protein regulates callose metabolism in rice pollen mother cells, thereby affecting the formation of pollen exine and pollen fertility. The invention provides a way for artificially controlling ricepollen fertility. It has important application value in the field of rice hybrid breeding.

The invention discloses a method for observing the deposition of callose of Agrostis stolonifera blade tissues based on a paraffin slice and an aniline blue fluorescence staining technology. The method comprises the following steps: 1, preparing a medicine; 2, making the paraffin slice sequentially through the steps of material drawing, fixation, dehydration, transparentizing, paraffin impregnation, embedding, slicing, slice flatting, slice drying, staining and slice sealing, staining the obtained slice by using a fluorescence staining agent, and observing and shooting the stained slice under a fluorescence microscope. The method for observing the deposition of callose of Agrostis stolonifera blade tissues based on the paraffin slice and the aniline blue fluorescence staining technology greatly shortens the experiment time of Agrostis stolonifera paraffin slice production, overcomes the difficulty in the production of the small and fine slice of an Agrostis stolonifera blade, breaks restriction brought by the material and environment factors, rapidly and highly-efficiently completes the paraffin slice production process, and finally obtains an excellent callose fluorescence microscopic observation result.

The invention discloses a method for dyeing and observing wheat tissue callose and belongs to the technical field of chemical plant tissue positioning. The method mainly comprises the following steps: (1) preparing a solution; (2) treating the material; (3) slicing; (4) dyeing materials; (5) producing sections; and (6) exciting through a laser excitation device, and observing and shooting through a fluorescence microscope. By adoption of a bare-handed slicing method, the wheat tissue slices which are manufactured in a bare-handed mode are dyed under the alkaline conditions by using the callose specifity fluorescent dye-water-soluble aniline blue, the sample slice manufacturing, dyeing and observing period is obviously shortened, and the callose is accurately positioned. The method is high in operability, the manufactured slices are conveniently observed, and the acquired image is clear.

The invention discloses an application of a Candidatus Liberibacter asiaticum effector as a target for screening anti-Candidatus Liberibacter asiaticum drugs, and finds that the Candidatus Liberibacter asiaticum flagellum matrix P-ring protein as the effector can cause significant callose deposition, and an inhibitor of the P-ring protein can be used for preventing and treating Candidatus Liberibacter asiaticum, and has a good application prospect in the field of prevention and treatment of Candidatus Liberobacter asiaticum. Meanwhile, the characteristics of small genome, simple culture, short life cycle and stable genetic manipulation of the saccharomyces cerevisiae are utilized, and the saccharomyces cerevisiae is used as a model organism for researching the effector of the bacterium CLas. Candidatus liberobacter asiaticum effector bacterium flagellum matrix P-ring protein is overexpressed in the saccharomyces cerevisiae, so that the inherent physiological function of the saccharomyces cerevisiae is disturbed, the phenotype of the saccharomyces cerevisiae is changed, and the proliferation of saccharomyces cerevisiae cells is inhibited. Meanwhile, transient expression in the Bensi tobacco leaves under agrobacterium tumefaciens mediation verifies and identifies that the gene can induce plant defense reactions such as local allergic necrosis of tobacco leaves and callose deposition.

The invention relates to a method for treating paddy rice tissues by using a prokaryotic expression product of a magnaporthe grisea effect protein gene and inducing generation of callose, belonging to the technical field of plant protection and biology. The method is an improvement on the prior art, comprises a prokaryotic expression technology which is the same as that of the conventional method, a protein purifying technology, the wavelength ranges of exciting light and emitted light of a used fluorescence microscope and an aniline blue dyeing method, and is characterized by comprising the following steps of: treating the root tissues of Nipponbare serving as a disease-resistant paddy rice variety with the prokaryotic expression product which is 1.0-10.0 mg / ml in concentration of the magnaporthe grisea effect protein gene is used for treating the root tissues for 12 hours; performing aniline blue dyeing; and directly observing the formation of callose under the fluorescence microscope. The method is easy, convenience, accurate and rapid; and a large amount of spontaneous fluorescence interference can be avoided effectively, and the situation of basic defense reaction of paddy rice can be obtained directly and qualitatively.

A method of treating cotton comprising: (i) contacting cotton with an aqueous wash liquor comprising water and an endo-β-1,3-glucanase enzyme; (ii) optionally rinsing the cotton; and (iii) drying the cotton. The wash liquor may contain surfactant. The cotton may be contacted with the aqueous wash liquor with agitation. The method may be for removal of callose from cotton. The method may be for the manufacture of a cotton-containing fabric having improved whiteness maintenance, soil repellency, reduced malodour, anti-wrinkle benefits, and / or improved drying.

The invention discloses a meloidogyne graminicola translation elongation factor Mg-eFF1A and the application thereof to the control of plant diseases. A genomic sequence of the Mg-eFF1A is as shown inSEQ ID NO.1, and an amino acid sequence is as shown in SEQ ID NO.2. The Mg-eFF1A is firstly discovered, the fact that the Mg-eFF1A can be secreted to the exterior of body of meloidogyne graminicola,and the Mg-eFF1A can activate an immune system of a host during invasion of plant-parasitic nematodes, induce the fundamental immune response of paddies and disease resistance to pathogen, activate PTI immune response, such as pathogenesis-related gene expression, callose accumulation and MAP kinase phosphorylation, of the paddies, and enhance the resistance of the paddies to meloidogyne graminicola and magnaporthe grisea. In addition, researches find that the Mg-eFF1A can induce plant OsCERK1 expression during invasion of the meloidogyne graminicola, and the researches open a direction and strategy with huge potential for the prevention and control of the plant-parasitic nematodes.

The present invention relates to a polypeptide, or a fragment thereof, capable of enhancing callose biosynthesis and / or accumulation, wherein at least one of the conserved amino acid residues selected from the group consisting of residue corresponding to R84 of SEQ ID NO: 1, residue corresponding to R1926 or SEQ ID NO: 1 and residue corresponding to P189 of SEQ ID NO: 1, of the polypeptide or a fragment thereof, is modified by a mutation selected from the group consisting of substitution and deletion.

The invention pertains to the technical field of agricultural biotechnology, and specifically relates to application of a Verticillium Wilt resistance-related gene GhSDH1-1. The gene GhSDH1-1 has a nucleotide sequence shown as SEQ ID No. 1. Being inoculated with a Verticillium Wilt pathogen Vd080 spore suspension, gene GhSDH1-1 silencing plants become more susceptible; and it is found by mechanismresearches that, in the silencing plants, synthesis of callose decreases, reactive oxygen species (ROS) reduces, and some defense genes subject to down-regulation. In addition, arabidopsis thaliana subjected to GhSDH1-1 gene transgenosis shows relatively strong resistance to Verticillium Wilt. Thus, the gene GhSDH1-1 is positively correlated with Verticillium Wilt resistance of cotton, and can beapplied in disease resistance breeding of the cotton.

The invention discloses a method for detecting generation of plant immunoreaction. The existing purification expression through prokaryotic expression products and the callose and reactive oxygen observation method are remained the same; the prokaryotic expression products of rice blast mycoprotein genes are adopted; 24 rice single-gene varieties containing different resistance genes, root organizations and sheaths of rice are processed according to the concentration of 5.0 mg / ml; the DAB dyeing and the aniline blue dyeing are implemented after 24 hours; the forming conditions of callose and reactive oxygen are directly observed through a fluorescence microscope; and the generation of the plant immunoreaction is judged through the forming conditions of the callose and the reactive oxygen. The method uses the prokaryotic expression products of purified effect protein genes to process the rice root organizations, and observes the generation of the callose and the reactive oxygen of the root organizations through the fluorescence microscope, so that the generation condition of the rice immunoreaction can be directly obtained, and the rice blast mycoprotein capable of leading a plurality of rice single-gene varieties containing different resistance genes and susceptible varieties to generate the immunoreaction is synchronously obtained.

The invention discloses a recombinant vector, a host cell and an application of an ustilaginoidea virens effector protein, and belongs to the technical field of biology. The recombinant vector is usedfor expressing an ustilaginoidea virens effect protein, and is obtained by inserting a coding gene of the ustilaginoidea virens effect protein to a position between EcoR I and Xho I restriction enzyme cutting sites of a pET-32a vector. The amino acid sequence of the ustilaginoidea virens effect protein is shown in the formula of SEQ ID NO: 1 in a sequence table and the nucleotide sequence of thecoding gene of the ustilaginoidea virens effect protein is shown in the formula of SEQ ID NO: 2 in the sequence table. According to the invention, the ustilaginoidea virens effect protein obtained byin vitro expression is sprayed on arabidopsis thaliana leaves so that the contents of active oxygen and callose in the arabidopsis thaliana can be obviously improved and the disease resistance of thearabidopsis thaliana to pathogenic bacteria Pseudomonas syringae pv.tomato (Pst) DC3000 can be improved. A novel way is provided for improving the plant resistance and inducing the plant defense reaction, and the recombinant vector has a wide application prospect in agricultural production.

The occurrence of diseases is an important factor restricting crop high yield. The fields with diseases usually have yields reduced by 20%-30%, the fields with serious diseases have the yields reduced by 50%-60%, and serious economic losses are caused. According to the invention, an over expression vector of OsNDPK1 gene (with the gene serial number of Os07g0492000) is constructed, and an over expression mutant of the OsNDPK1 gene is obtained through agrobacterium transformation. Experiments show that: the OsNDPK1 gene can improve resistance of plants to bacteria PstDC3000 and fungus botrytis cinerea BC22, can regulate generation of callose in plant bodies, and can induce expression of anti-disease genes NPR1 and PR1. Analysis and identification of OsNDPK1 gene functions provide theoretical basis and production and practice bases for obtaining of new varieties of anti-disease transgenic plants.

A method of treating cotton comprising: (i) contacting cotton with an aqueous wash liquor comprising water and an endo-β-1,3-glucanase enzyme; (ii) optionally rinsing the cotton; and (iii) drying the cotton. The wash liquor may contain surfactant. The cotton may be contacted with the aqueous wash liquor with agitation. The method may be for removal of callose from cotton. The method may be for the manufacture of a cotton-containing fabric having improved whiteness maintenance, soil repellency, reduced malodour, anti-wrinkle benefits, and / or improved drying.

The invention discloses a method for improving the double-flower rate and regulating the flowering period of paphiopedilum callose. The method comprises the following step of injecting a gibberellin solution into the base part of a paphiopedilum callose plant with 4-5 leaves before flowering. On the basis of improving the cultivation technology, the gibberellin (GA3) injection method is adopted, the double-flower rate of the paphiopedilum callose can be greatly increased, the flowering period is 1-2 months earlier, and the flowering rate and the flowering uniformity are improved. Reports on paphiopedilum flowering period regulation by adopting a gibberellin injection method do not exist at home and abroad, and the method can fill the blank at home and abroad and has a positive promotion effect on promoting the development of the paphiopedilum industry in China. The method is feasible and practical in technology and has high application value.

A mutant male-sterile soybean line designated msMOS is disclosed. This shows genic male sterility at a new locus designated 'msMOS'. Male sterility appears to be the result of impaired tetrad callose degeneration due to an absence of functional callase enzyme.

Cleaning compositions comprising endo-β-1,3-glucanase enzyme and cleaning adjunct. Methods of treating surfaces including fabrics by contacting the surface with an aqueous was liquor having the cleaning composition therein. The compositions and methods are particularly for cleaning cotton fabrics. The compositions and methods are particularly for removal of soils containing callose, curdlan, pachyman, scleroglucan or schizophyllan. The compositions and methods are particularly for improving whiteness of a fabric, improved soil removal from a fabric, for malodour removal from a fabric, for anti-wrinkle benefits and / or for improved drying of a fabric.

The invention belongs to the field of plant biotechnology and relates to a kit and method for marking a microfilament cytoskeleton of a pollen tube after apple pollination. The invention firstly provides a kit for marking the microfilament cytoskeleton of the pollen tube after the apple pollination, comprising a style fixer reagent A, a style washing liquid reagent B, a microfilament dye reagent Cand a callose immunofluorescence reagent D. Then the kit is applied for marking the microfilament cytoskeleton according to the following steps: 1) taking a pollinated style; 2) fixing the style by adopting the style fixer reagent A; 3) washing the style by adopting the style washing liquid reagent B; 4) dyeing the style by adopting mixed solution of the microfilament dye reagent C and the callose immunofluorescence reagent D; and 5) dropwise adding a fluorescence-quenching-resistant agent to the style, and observing the microfilament cytoskeleton of the pollen tube in the style under a laserscanning confocal microscope. The kit and the method which are provided by the invention realize rapid, sensitive, accurate and simple observation on a structure and an arrangement mode of the microfilament cytoskeleton in an apple pollen tube.

Plasmodesmal resident components are identified, characterized and isolated. Compositions comprising these components are described and methods of use thereof for plasmodesmal flux modulation and targeting are enabled. In a first embodiment, a novel plasmodesmal receptor-like protein, referred to herein as pldp1, reveals signals sufficient for targeting to plasmodesmata via the secretory pathway. In the second embodiment a novel plasmodesmal protein that is anchor into the external face of the plasma membrane and binds to callose is provided.

The present invention relates to a polypeptide, or a fragment thereof, capable of enhancing callose biosynthesis and / or accumulation, wherein at least one of the conserved amino acid residues selected from the group consisting of residue corresponding to R84 of SEQ ID NO: 1, residue corresponding to R1926 or SEQ ID NO: 1 and residue corresponding to P189 of SEQ ID NO: 1, of the polypeptide or a fragment thereof, is modified by a mutation selected from the group consisting of substitution and deletion.

Provided herein is Bacillus amyloliquefaciens strain PMB05 used in the field of plant disease control. Inoculation of plants with the strain can increase immune responses induced by detection of fungi and / or bacteria in plant, for example, increase the production of ROS and deposition of callose. Also, the strain has the effect of promoting plant growth. Therefore, the strain and its culture filtrate of the present invention can boost plant disease resistance and promote plant growth, which can be extensively used to control plant diseases and have the potential of turning into a commodified biological control agent.

A method for producing non-cellulosic callose fiber by using plant protoplast, which imposes less burden to the environment with reduced energy consumption compared to conventional natural fibers is provided; it comprises the addition of an inorganic ion to a plant protoplast cultivation system, which leads the plant protoplast to produce non-cellulosic callose fiber.

Provided herein is Bacillus amyloliquefaciens strain PMB05 used in the field of plant disease control. Inoculation of plants with the strain can increase immune responses induced by detection of fungi and / or bacteria in plant, for example, increase the production of ROS and deposition of callose. Also, the strain has the effect of promoting plant growth. Therefore, the strain and its culture filtrate of the present invention can boost plant disease resistance and promote plant growth, which can be extensively used to control plant diseases and have the potential of turning into a commodified biological control agent.

The invention discloses a regulator for increasing rice dietary fibers and application thereof, and belongs to the technical field of crop regulators, the regulator comprises the following components by mass: 8-15% of a growth regulator, 3-6% of an organic matter, 1-3% of an inorganic salt, and the balance of water, totaling 100%; wherein the growth regulator is selected from at least one of compound sodium nitrophenolate, ethephon, chloromethylsilatrane, forchlorfenuron and diphenylurea sulfonic calcium; the organic matter is selected from at least one of chitin and compound amino acid; the inorganic salt is selected from at least one of magnesium sulfate, ferrous sulfate, potassium chloride, calcium nitrate, borax and potassium silicate; the invention further discloses a preparation method and application of the regulator. By applying the regulator, the dietary fiber content and disease resistance of rice can be remarkably improved, accumulation of reactive oxygen species (ROS) and callose of rice leaves can be promoted, and finally the purpose of reducing application of chemical fertilizers and pesticides is achieved.

The present invention "a trichoderma hydrophobin Thfb6 and its strain, plant ISR elicitor and excitation method" belongs to the field of molecular biology. The amino acid sequence of the Trichoderma hydrophobin Thfb6 is shown in SEQ ID NO.1. Based on the Trichoderma hydrophobin Thfb6, the present invention also provides a plant ISR elicitor and its stimulating method, a TMV resistance inducer and its inducing method. The trichoderma hydrophobin Thfb6 of the present invention can cause active oxygen bursts in plant leaves, induce cell extracellular alkalization, callose and phenolic substance accumulation and other early defense reactions, and improve stress resistance enzyme activities such as PAL, POD and PPO in leaves Improve and induce the expression of disease process-related protein gene PR‑1b, systemic resistance marker gene NPR1 and defense response key enzyme gene PAL, and induce tobacco to improve disease resistance to tobacco mosaic disease, and at the same time induce cucumber resistance to Fusarium wilt sex.

The invention belongs to the field of agricultural biotechnology, and in particular relates to the cotton Verticillium wilt resistance related gene GhABC and its encoded protein and application. The nucleotide sequence of the cotton verticillium wilt resistance-related gene GhABC of the present invention is shown in SEQ ID No.2, and the amino acid sequence of its encoded protein is shown in SEQ ID No.1. After the gene silencing plants are inoculated with the spore suspension of the cotton Verticillium dahliae pathogen Vd080, the silencing plants are more susceptible to the disease. Through mechanism research, it is found that the synthesis of xylem and callose in the silencing plants decreases, the ROS decreases, and the expression of some defense genes is down-regulated. It proved that GhABC is positively correlated with cotton resistance to Verticillium wilt, and GhABC can be used as a candidate gene for cotton breeding for disease resistance.