Method for observing deposition of callose of Agrostis stolonifera blade tissues based on paraffin slice and aniline blue fluorescence staining technology

Abstract

The invention discloses a method for observing the deposition of callose of Agrostis stolonifera blade tissues based on a paraffin slice and an aniline blue fluorescence staining technology. The method comprises the following steps: 1, preparing a medicine; 2, making the paraffin slice sequentially through the steps of material drawing, fixation, dehydration, transparentizing, paraffin impregnation, embedding, slicing, slice flatting, slice drying, staining and slice sealing, staining the obtained slice by using a fluorescence staining agent, and observing and shooting the stained slice under a fluorescence microscope. The method for observing the deposition of callose of Agrostis stolonifera blade tissues based on the paraffin slice and the aniline blue fluorescence staining technology greatly shortens the experiment time of Agrostis stolonifera paraffin slice production, overcomes the difficulty in the production of the small and fine slice of an Agrostis stolonifera blade, breaks restriction brought by the material and environment factors, rapidly and highly-efficiently completes the paraffin slice production process, and finally obtains an excellent callose fluorescence microscopic observation result.

Description

technical field [0001] The invention relates to a rapid detection technology for microscopic tissue sections and histochemical components of callose, in particular to a method for observing callose deposition in creeping bentgrass leaf tissues based on paraffin sections and aniline blue fluorescent staining. Background technique [0002] Callose is a linear β-1,3 glucan with 1,6 branches, and becomes a jelly-like structure that becomes thicker and shorter after heating. During plant development, callose can be found in many places, including cell plates, pollen, pollen tubes, seeds, leaves and stems, epidermis, sieve plates, plasmodesmata, secondary walls during xylem formation, etc. Callose has the physical and physiological properties of rapid polymerization and depolymerization. In addition, as part of the stress response, it is synthesized rapidly and in large quantities when plants are injured, infected by pathogenic bacteria, or subjected to various stresses, in respo...

AI Technical Summary

Problems solved by technology

However, the conventional paraffin sectioning method takes a long time, and it takes at least one week to make a permanent slice specimen from taking the material and fixing it, and it is limited by many factors in practical application, and some harmful substances to the human body are involved in the slice making process. The use of drugs and long-term experiments will endanger the health of experimenters and cause environmental pollution

The most important thing is that the growth status of creeping bentgrass cannot be detected in time because of the long experiment period and remedial measures can be taken in time for the disease