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Method for quickly identifying cross-compatibility of rose hybrida

An identification method and affinity technology, which is applied in the field of plant reproductive biology, can solve the problems of wasting breeding resources, reducing breeding efficiency, and infertility in hybridization, and achieve the effects of reducing the waste of breeding resources, improving breeding efficiency, and strong operability

Inactive Publication Date: 2012-07-11
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The hybrid incompatibility of rose has the following manifestations: during cross-pollination, 1) the pollen grains cannot germinate on the stigma; 2) although the pollen grains can germinate on the stigma, the pollen tube cannot pass through the stigma or reach the style Embryo sac; 3) Although the pollen tube can reach the embryo sac, the sperm released by it cannot fuse with the egg cell to form a zygote, which eventually leads to infertile hybridization
In the process of conventional cross-breeding, it is only possible to identify whether the parents are compatible by observing whether the ovary expands after pollination, which usually needs to be observed about 15-20 days after pollination, which not only wastes a lot of breeding resources, It also reduces breeding efficiency, which is not conducive to the development of high-quality rose flower industry

Method used

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  • Method for quickly identifying cross-compatibility of rose hybrida
  • Method for quickly identifying cross-compatibility of rose hybrida
  • Method for quickly identifying cross-compatibility of rose hybrida

Examples

Experimental program
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Effect test

Embodiment 1

[0035] The Chinese rose in the present embodiment 1 refers to Rosaceae Rosa ( Rosa The cultivars (cultivar) in Linnaeus) were subjected to the following steps:

[0036] 1) Collection and preservation of pollen: Select the vigorously growing plants, select the flowers to be opened, and mark them, place the anthers picked from the male parent flowers in a ventilated place at 20°C to dry in the shade, and store the scattered pollen at 4°C ,stand-by;

[0037] 2) Detasseling: At 15:00 in the afternoon, select the flowers of the female plant that will bloom the next day for detasseling, and then bagging, because the stamens are not yet fully developed at this time, and pollen will not be scattered during emasculation;

[0038] 3) Artificial pollination: At 9:00 in the morning, first remove the bagging on the female plant, dip a proper amount of scattered pollen with a brush, smear pollination on the stigma of the female parent, then bag it, keep it for 30 hours, and put it on the...

Embodiment 2

[0048] The Chinese rose in the present embodiment 2 refers to Rosaceae Rosa ( Rosa The cultivars (cultivar) in Linnaeus) were subjected to the following steps:

[0049] 1) Collection and storage of pollen: Select the vigorously growing plants, select the flowers to be opened, and put them on a sign, place the anthers picked from the male parent flowers in a ventilated place at 25°C to dry in the shade, and store the scattered pollen at 4°C ,stand-by;

[0050] 2) Detasseling: At 17:00 p.m., select the flowers that will bloom the next day on the female parent plant for detasseling, and then bagging, because the stamens are not yet fully developed at this time, pollen will not be scattered during detasseling;

[0051] 3) Artificial pollination: At 10:00 in the morning, first remove the bagging on the female plant, use a brush to dip in an appropriate amount of scattered pollen, smear pollination on the stigma of the female parent, then bag it, keep it for 48 hours, and put it on...

Embodiment 3

[0061] The Chinese rose in the present embodiment 3 refers to Rosaceae Rosa ( Rosa The cultivars (cultivar) in Linnaeus) were subjected to the following steps:

[0062] 1) Collection and preservation of pollen: Select the vigorously growing plants, select the flowers to be opened, and mark them, place the anthers picked from the male parent flowers in a ventilated place at 22°C to dry in the shade, obtain scattered pollen, and store at 4°C ,stand-by;

[0063] 2) Detasseling: At 15:30 in the afternoon, select the flowers that will bloom the next day on the female plant for detasseling, and then bagging, because the stamens are not yet fully developed at this time, pollen will not be scattered during detasseling;

[0064] 3) Artificial pollination: At 9:40 in the morning, first remove the bagging on the female plant, dip a proper amount of scattered pollen with a brush, smear pollination on the stigma of the female parent, then bag it, keep it for 38 hours, and put it on the ...

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Abstract

The invention provides a method for quickly identifying cross-compatibility of rose hybrida. The method is characterized by comprising the following steps of: collecting and storing pollen, performing castration, performing artificial pollination, cutting a sample to be identified, fixing the cut sample in FAA stationary liquid, transferring the cut sample to a 70 percent ethanol solution after 24 hours, taking out for washing, placing in an 8mol / L NaOH solution, performing water bath at the temperature of 55 to 60 DEGC for 15 to 20 minutes, and then standing at the temperature of 20 to 28 DEGC for 3 to 3.5 hours, soaking into water for at least 1 hour, transferring to a 0.1 percent dye solution of aniline blue, dyeing for 2 to 3 hours, observing stigma callose deposition, pollen germination and growth of pollen tubes by using a fluorescence microscope to identify the cross-compatibility of rose hybrida. By the method, the cross-compatibility of rose hybrida can be identified within 30 to 48 hours; and moreover, the method is simple and easy to operate and can effectively reduce the waste of breeding resources and improve breeding efficiency.

Description

[0001] technical field [0002] The invention relates to the field of plant reproductive biology, in particular to a rapid identification method for hybrid compatibility of roses. Background technique [0003] Rose (Rosa hybrida) is the largest ornamental plant in the world. So far, there are more than 35,000 varieties of rose in the world. Most rose varieties are bred through long-term distant and close hybridization. With the implementation of the 1991 text of the UPOV Convention, hybrid breeding will become the only means of cultivating new varieties of roses. It is very important to study the hybrid compatibility of paired parents for the selection of rose varieties, especially the application research of breeding core germplasm resources, and use distant hybridization to screen resistant varieties. Hybrid compatibility of roses is controlled or affected by genetic kinship, genetic genes, chromosomal ploidy, etc. On the basis of molecular breeding methods to study gene...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 刘红明王其刚唐开学张颢
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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