The invention discloses a method for loading a bioactive protein on a hydrophobic stent material. Through the effect of bonding with the hydrophobic end of an amphiphilic protein HFBI, the bioactive protein is loaded onto the degradable aliphatic polyester porous fiber stent material, the bioactive protein is then bonded with the hydrophobic end of a hydrophobin, and thereby a novel bioactive protein-loaded tissue engineering stent material is prepared. The stent material remarkably improves the hydrophilic property of hydrophobic materials, the biocompatibility is good, and the invention proves that the stent material has the effect of promoting cell migration and vascularization. The method can be used for loading a macromolecular protein on the surface of a hydrophobic material, and while the biocompatibility of the material is improved, the activity of the loaded protein is kept, so that the hydrophobic material can play a corresponding biological role.