Establishment of method for screening and identifying new species of different types of edible fungi

Abstract

The invention provides a new method for screening and identifying genetic breeding new species of edible fungi, which solves the problems of obvious screening mark shortage, a waste of time and heavy work load during identifying whether the edible fungi or the new species contains manganese peroxidase or lignin peroxidase or not and slow qualitative identification and monitoring in the prior art. The new method comprises the following steps of: using aniline blue as one of materials for preparing a solid culture medium; inoculating a strain of the edible fungi to the solid culture medium; viewing the color change condition of the aniline blue solid culture medium after culturing; and identifying whether the edible fungi contains the manganese peroxidase or the lignin peroxidase or the manganese peroxidase and the lignin peroxidase or not. The new method can directly, conveniently and flexibly qualitatively judge whether the edible fungi contains the manganese peroxidase or the lignin peroxidase or not, is especially suitable for qualitatively detect two different types of wood-rotting fungi or new fused strains of other fungi and has low detection cost.

Description

technical field [0001] The invention belongs to a qualitative identification method for manganese peroxidase or lignin peroxidase in edible fungi. Background technique [0002] Lignin peroxidase and manganese peroxidase can be used for pulp biological bleaching, degradation of organic pollutants (such as soil bioremediation, biological treatment of printing and dyeing wastewater and chemical wastewater), etc., and their applications are very extensive. There are more and more studies on the types and new varieties of edible fungi producing manganese peroxidase and lignin peroxidase. There is a lack of screening markers for varieties, and the screening of fusion strains is a lot of work, and identification is time-consuming and laborious, especially for the fusion strains of different types of edible fungi that contain manganese peroxidase and lignin peroxidase, rapid qualitative identification and monitoring cannot be performed. Contents of the invention [0003] The purp...

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Problems solved by technology

[0003] The purpose of the present invention is to provide a new method for screening and identification of new varieties of different types of edible fungi, to solve the lack of obvious screening in the identification of whether there are manganese peroxidase or lignin peroxidase in edible fungi or new varieties. Signs, time-consuming, heavy workload, and cannot be quickly qualitatively identified and monitored. This method can directly, conveniently and sensitively determine whether there is manganese peroxidase or lignin peroxidase in edible fungi, and is especially suitable for To qualitatively detect the fusion of two different types of wood rot fungi or other fungal strains, the detection cost is low

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