Screening method of high-performance hydrolytic bacterial strain of waste Baijiu distiller grains

Abstract

The invention discloses a screening method of a high-performance hydrolytic bacterial strain of waste Baijiu distiller grains. The screening method is characterized by comprising the following steps:step I, directly separating and culturing fungus from effective fungus that possibly hydrolyzes waste Baijiu distiller grains of relevant waste hydrolytic Baijiu distiller grains in a bacterial strainlibrary in a waste Baijiu distiller grains stacking environment; step II, performing the primary screening for the screened fungus successively by virtue of a sodium carboxymethylcellulose-Congo redculture medium, PDA-aniline blue culture medium, and a xylan-Congo red culture medium by adopting the single polymer which is difficult to hydrolyze in the waste Baijiu distiller grains as an exclusive carbon source; step III, culturing the bacterial strain in a waste distiller grain solution adopting the waste Baijiu distiller grain as an exclusive nutritional substance, and determining the capability of the bacterial strain for the waste distiller grains; step IV, performing the enzyme activity test for the composite bacterial strain in the waste Baijiu distiller grain solution, and screening the bacterial group with synergistic effect and high enzyme activity, thus obtaining the high-performance composite hydrolytic bacterial group; and step V, comparing enzyme activity and biomass hydrolytic capability of the high-performance composite bacterial strain with that of the disclosed mutant bacterial strain having the high-performance hydrolytic cellulose.

Description

technical field [0001] The present invention relates to the optimization research of the scheme of hydrolyzing liquor and discarding grains, in particular to the screening research of two groups of new high-efficiency hydrolyzing liquor and discarding grains compound bacterial groups. technical background [0002] Liquor distilled grains are the husks and residues of grains that are poor in nutrition after repeated fermentation and distillation to extract alcohol. That is, the content of distiller's grains is high in moisture and lignocellulose (cellulose, hemicellulose, lignin), and the content of crude protein and crude fat is low. Because the lost grains have less nutrients and lignocellulose is difficult to hydrolyze, it cannot be as effective as ordinary distiller's grains Therefore, the transformation of waste waste is greatly restricted. Moreover, the waste waste has high acidity and high moisture content, which is very easy to spoil and deteriorate, causing pollutio...

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Problems solved by technology

[0004] The technical problem to be solved by the present invention: In view of the situation of high acidity, low nutrition, low usable value, and environmental pollution of discarded grains, provide a kind of high-efficiency microbial group to improve the hydrolysis rate of discarded grains, reduce the storage space of waste residues, reduce the The technical solution for waste residue treatment costs promotes the maintenance of a good ecological environment, achieves sustainable recycling of liquor waste waste, and lays the foundation for the government's goal of zero emissions and pollution-free advocacy of enterprises

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