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Thermophilic aerophilic thiamine-decomposing bacillus, microbial inoculum and application of microbial inoculum

A bacillus and sulfur-resolving technology, applied in the field of microorganisms, can solve problems such as low decomposition efficiency

Active Publication Date: 2021-01-22
江苏进化树生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current use of bacteria to decompose lignin has the problem of low decomposition efficiency

Method used

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  • Thermophilic aerophilic thiamine-decomposing bacillus, microbial inoculum and application of microbial inoculum
  • Thermophilic aerophilic thiamine-decomposing bacillus, microbial inoculum and application of microbial inoculum
  • Thermophilic aerophilic thiamine-decomposing bacillus, microbial inoculum and application of microbial inoculum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: the isolation culture of soil microorganism LDH-C

[0033] 1. Sample collection: In June 2018, I went to Yichun City, Heilongjiang Province, and took soil samples within 20cm of forest rotting wood. I took soil samples from the surface layer, 5cm and 10cm depths, and collected the soil with a sealed bag. After the collection, I used it in the experiment The three soil samples were mixed indoors, and the mixed soil samples were divided into three parts, which were used as three replicates. After being quick-frozen in liquid nitrogen, they were stored in a -80°C refrigerator for later use.

[0034] 2. Soil enrichment and separation

[0035] Accurately weigh 1g of the mixed soil sample, put it into a 250ml Erlenmeyer flask filled with 100ml of double distilled water, put it into a shaker, shake it at 120rpm / min for 30 minutes, let it stand for 1 hour, and take the supernatant according to 10% of the inoculum was inoculated in the MSM medium with guaiacol (1m...

Embodiment 2

[0042] The 16SrDNA sequence analysis of embodiment 2LDH-C

[0043] The strain LDH-C was sent to Shanghai Passino Biotechnology Co., Ltd. for 16SrDNA sequencing analysis, so as to identify the strain.

[0044] Bacterial species identification results: Blast sequence comparison of the sequenced strains on NCBI showed that the sequence similarity between strain LDH-C and Aneurinibacillus thermoaerophilus strain DSM 10154 was 99.86%, and the strain LDH could be identified accordingly -C belongs to Aneurinibacillusthermoaerophilus in molecular phylogenetic taxonomy.

[0045] The 16SrDNA sequence of strain LDH-C is shown in SEQ ID NO.1, image 3 A phylogenetic tree based on the 16S rDNA sequence of the strain LDH-C. from image 3 It can be seen that the strain closest to LDH-C is Aneurinibacillus thermoaerophilus strain DSM 10154, indicating that LDH-C belongs to Aneurinibacillus thermoaerophilus in molecular phylogenetic taxonomy.

Embodiment 3

[0046] Example 3 Alkali lignin decomposition ability of lignin decomposing bacteria LDH-C.

[0047] The liquid-cultured lignin-decomposing bacteria LDH-C was inoculated at a 10% inoculum size in a liquid medium with alkali lignin as the only carbon source (adding alkali lignin 3g / L on the basis of MSM medium) for three cultures. For each repetition, the culture medium inoculated with the same amount of sterile water was used as a blank, and cultured in a constant temperature incubator at 30, 40, 50, and 60°C for 24 hours.

[0048] Drawing of alkali lignin standard curve: Weigh alkali lignin, prepare alkali lignin solutions with different concentration gradients (addition amount of alkali lignin is 0.040625, 0.08125, 0.1625, 0.325, 0.75, 1.5, 3g / L), centrifuge Take the supernatant, and measure the absorbance value of different concentrations of alkali lignin at 280nm. Taking the alkali lignin concentration as the abscissa and the absorbance as the ordinate to make a graph, obt...

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Abstract

The invention relates to thermophilic aerophilic thiamine-decomposing bacillus, a microbial inoculum and application of the microbial inoculum. The bacillus can well grow at the temperature of 30-60 DEG C. In an aniline blue decoloration test, a decoloration ring with the diameter of 5cm can be generated within 24h. The microbial inoculum has the advantages of high lignin degradation rate, high temperature resistance, short growth period and the like when being applied to lignin degradation, and particularly can be used for strain resources of high-temperature straw compost.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a thermophilic aerophilic thiamine-lytic bacillus, a bacterial agent and an application thereof. Background technique [0002] Lignin is a renewable resource second only to cellulose in nature, and it is one of the main components of plant cell walls, accounting for 15%-35%. The decomposition of lignin has a crucial impact on the earth's carbon cycle, but because of its complex chemical structure and heterogeneity, it has been treated as a waste and has not been well utilized. At the same time, its decomposition and value-added become a huge challenge. Lignin is a part of lignocellulose. In the vascular tissue of the cell wall of higher plants, lignin, hemicellulose and cellulose fibrils are closely intertwined and embedded through covalent and non-covalent bonds to form lignocellulose. . Lignin is a three-dimensional network of phenolic, non-crystalline pol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F17/20C12R1/01
CPCC12N1/20C05F17/20C12R2001/01C12N1/205Y02W30/40
Inventor 王伟东
Owner 江苏进化树生物科技有限公司
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