Artificially synthesized cationic peptide and applications thereof in preparing anti-tumor drug

Abstract

The invention discloses an artificially synthesized cationic peptide (named as AIK) and applications thereof in preparing an anti-tumor drug. The amino acid sequence of the cationic peptide is shown as SEQ ID NO.1. A research shows that the AIK has an obvious inhibition effect on various solid tumor cells, especially leukemic cells, cells of pulmonary giant cell cancer and hepatoma ascites cells, the anti-tumor activity is possibly combined an anionic acceptor on the surface of tumor cells so as to induce the tumor cells to be subjected to apoptosis and necrosis, and the anti-tumor activity is higher than that of the anti-tumor peptide molecules reported by other researchers. More importantly, the artificially synthesized 25-peptide drug molecules have higher specificity on the tumor cells. After the anti-tumor drug is locally administrated by subcutaneous tumor-bearing mice, the subcutaneous growth of liver cancer cells H22 of the tumor-bearing mice can be obviously inhibited and toxic and side effects are lower than chemotherapeutic drugs amethopterin. The artificially synthesized cationic peptide can provide theoretical basis and experimental materials for further researching and developing polypeptide anti-tumor new drugs and small-size targeted anti-tumor new drugs.

Description

technical field [0001] The invention relates to an artificially synthesized cationic peptide and its use in preparing new anticancer drugs, in particular to an artificially synthesized cationic 25 peptide and its use in inducing tumor cell apoptosis. The invention belongs to the field of biotechnology. Background technique [0002] Malignant tumors are a type of disease that seriously threatens human health and life. Although the existing anti-tumor drugs have certain curative effects on most tumors, they still have low treatment efficiency, poor selectivity, large toxic and side effects, and easy production of tumor cells. issues of drug resistance. Therefore, it is still an urgent task to find anti-tumor drugs with high efficiency, low toxicity and strong specificity from different ways. Anti-tumor polypeptide drugs have small molecular weight (generally less than 50 amino acids), high activity, easy penetration and absorption, and low immunogenicity. Its structure is s...

AI Technical Summary

Problems solved by technology

[0013] In order to overcome the shortcomings of existing polypeptide anticancer drugs such as low antitumor activity, large molecular weight, and poor tissue penetration, the inventors of the present invention discovered a synthetic The cationic 25 peptide named as AIK, its amino acid sequence is shown in SEQ ID NO.1, its isoelectric point (pI) is 12.96, and its theoretical molecular weight (MW) is 3456.35Dalton

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