42 results about "Ovarian cell" patented technology

The invention discloses a C-shaped aftosa subunit genetic engineering vaccine and a preparation method thereof. The C-shaped aftosa subunit vaccine has the main component of a mixture formed by C-shaped aftosa structure protein VP1 and VP3 according to the protein content ratio being 1 / 1, wherein the C-shaped aftosa structure protein VP1 and VP3 is eukaryotic expression products of C-shaped aftosa virus gene VP1 and VP3, in particular to products of C-shaped aftosa antigenic gene VP1 and VP3 expressed in a Chinese hamster ovarian cell (CHO / dhfr-cell) expression system.

Glycosylated Antitumor Ether Lipids (GAELs) kill cancer cells by a nonapoptotic pathway which is an attractive strategy to avoid resistance. To further optimize the antitumor effect, we prepared various analogs of di-, and tri-cationic GAEL analogs differing in the nature of the sugar (D-glucose or L-glucose), the anomeric linkage as well as position of the glycerolipid moiety. The di- and tri-cationic GAELs were synthesized and their in vitro anticancer properties were evaluated against drug resistant and aggressively growing cancer cell lines derived from human breast, prostate, pancreatic and ovarian cancers. The most potent dicationic GAEL analogs were also studied against cancer stem cells obtained from breast BT 474, prostate DU145 and ovarian A2780cp cell lines. Our results indicate that the number of positive charges, the position of the amino substituents and the nature of the sugar have significant effects on the anticancer activities of these compounds. The most active analog kill 50% of the cells at concentration range of 0.5-5 μM and 90% of the cells at the concentration of 1-10 μM depending on type of cancer cells.

The present invention provides a high-yielding baculovirus pupae ovary cell line of Oriental Armyworm. The name of the cell line is IOZCAS-Myse-1, and its preservation number is CGMCC No.17282. The present invention provides a method for constructing the O. orientalis pupal ovary cell line of the high-yielding baculovirus. The method comprises the following steps: step 1) obtaining the ovary tissue of the oriental armyworm; step 2) cultivating the ovary tissue of the oriental armyworm, until The proliferated cells are filled with the culture flask, wherein the tissue is closely attached to the bottom of the cell culture flask; step 3) take the newly proliferated single cell and continue to culture until the cell is passed down; the whole process is carried out under sterile conditions . The oriental armyworm pupal ovary cell line with high yield of baculovirus provided by the present invention can be used to replicate baculovirus for large-scale production of baculovirus or baculovirus insecticide; the cell line can also be applied to construct Baculovirus expression vector system for expressing proteins of commercial or scientific value.