Product
Patsnap Eureka
For R&D, Patsnap Eureka makes reading and utilizing patents & technical documents easy.
Patsnap Eureka AIR
Designed for self-driven R&D workflows. Generate viable solutions, solve complex R&D challenges, empower your innovation with AI.
Patsnap Eureka Materials
Designed for material experts only. Revolutionize your material R&D, from search, analyze, to developing new materials.
Feature
TechResearch
Generate reliable direction feasibility study reports for your R&D in just a few steps.
TechSeek
Discover and master advanced knowledge NOW. Basics, ideas, possibilities, all at once.
TechMind
As an expert in R&D Theories, TechMind can generates customized viable solutions instantly.
TechRisk
Analyze your overall solution with one click, know your potential R&D risks in advance.
TechMonitor
Get weekly tech updates, stay abreast of the latest tech innovations and key insights.
Patents
Literature
Hiro is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
116 results about "Zona pellucida" patented technology
Efficacy Topic
Property
Owner
Technical Advancement
Application Domain
Technology Topic
Technology Field Word
Patent Country/Region
Patent Type
Patent Status
Application Year
Inventor
The zona pellucida (plural zonae pellucidae, also egg coat or pellucid zone) is a glycoprotein layer surrounding the plasma membrane of mammalian oocytes. It is a vital constitutive part of the oocyte. The zona pellucida first appears in unilaminar primary oocytes. It is secreted by both the oocyte and the ovarian follicles. The zona pellucida is surrounded by the corona radiata. The corona is composed of cells that care for the egg when it is emitted from the ovary.
Pluripotent stem cells derived without the use of embryos or fetal tissue
InactiveUS20030113910A1New breed animal cellsArtificial cell constructsPluripotential stem cellGerm layer
Owner:STEMA
Method of nuclear transfer
InactiveUS20080092249A1Increase the number of cellsImprove pregnancy rateRecombinant DNA-technologyFermentationNuclear transferSomatic cell
The present invention relates to nuclear methods and embryos developed therefrom. In particular, the present invention relates to a method of nuclear comprising the step of transferring a somatic cell nuclei into a zona pellucida-free, enucleated oocyte.
Owner:MONASH UNIV
Isolation of inner cell mass for the establishment of human embryonic stem cell (hESC) lines
InactiveUS7294508B2Easy to useAvoid possibilityMammal material medical ingredientsDead animal preservationGerm layerHuman embryonic stem cell line
A method for isolating an inner cell mass comprising the steps of immobilizing a blastocyst stage embryo having a zona pellucida, trophectoderm, and inner cell mass, creating an aperture in the blastocyst stage embryo by laser ablation, and removing the inner cell mass from the blastocyst stage embryo through the aperture. The aperture is through the zona pellucida and the trophectoderm. The laser ablation is acheived using a non-contact diode laser. The inner cell mass removed from the blastocyst stage embryo is used to establish human Embryonic Stem Cell lines.
Owner:RELIANCE LIFE SCI PVT
Isolation of inner cell mass for the establishment of human embryonic stem cell (hESC) lines
InactiveUS20030104616A1Ensure purityMinimize contaminationMammal material medical ingredientsArtificial cell constructsMidblastulaStem cell line
A method for isolating an inner cell mass comprising the steps of immobilizing a blastocyst stage embryo having a zona pellucida, trophectoderm, and inner cell mass, creating an aperture in the blastocyst stage embryo by laser ablation, and removing the inner cell mass from the blastocyst stage embryo through the aperture. The aperture is through the zona pellucida and the trophectoderm. The laser ablation is acheived using a non-contact diode laser. The inner cell mass removed from the blastocyst stage embryo is used to establish human Embryonic Stem Cell lines.
Owner:RELIANCE LIFE SCI PVT
Epitope minimal motif peptides of human zona pellucida protein-4 and extended short peptides and application thereof
The invention belongs to the technical field of biomedicine and biological detection, in particular to two linear epitope minimal motif peptides capable of being identified by human zona pellucida protein-4 (ZP4) monoclonal antibodies MA-1662 and MA-1671 on human ZP4 and extended eight peptides and application thereof. The invention provides two candidate epitopes which can be used for developing human immune birth control recombinant multi-epitope vaccines, and the amino acid sequences of the candidate epitopes are shown as SEQ ID No. 1 and marked as huZP4147-151 or shown as SEQ ID No.2 and marked as huZP4256-260; and the invention also provides candidate epitopes which can be used for developing novel high-specificity high-sensitivity recombinant multi-epitope detection antigens for diagnosing serum ZP antibodies of ZP autoimmune infertile women, and the amino acid sequences of the candidate epitopes are shown as SEQ ID No.3-SEQ ID No.6.
Owner:SHANGHAI INST OF PLANNED PARENTHOOD RES +1
Albumen microsphere conjugate for detecting acrosin activity and preparation method and application of albumen microsphere conjugate
InactiveCN106146642AAccurate evaluationRealize synchronous detectionSerum albuminFluorescence/phosphorescenceMicrosphereProtein labeling
The invention discloses an albumen microsphere conjugate for detecting acrosin activity and a preparation method and application of the albumen microsphere conjugate. The albumen microsphere conjugate is characterized in that monodisperse micron microspheres obtained by combining fluorescein and the acrosome reaction substrate, namely zona pellucida protein are prepared, the acrosin activity is detected by aid of change in fluorescence intensity, and the specific steps include preparation of monodisperse microspheres modified through surface carboxylation or amination, surface modificaction, fluorescence labeling of protein polypeptide, conjugation of fluorescent protein polypeptide and functional microspheres and detection of the acrosin activity. The albumen microsphere conjugate has the advantages that protease digestion on the microspheres is realized through the polymer microsphere technology and the protein labeling and conjugating technology, and enzymatic activity is evaluated; the albumen microsphere conjugate is mainly applied to flow detection of the acrosin activity, synchronous detection of an acrosome reaction and the acrosin activity can be realized, and acrosome functions can be evaluated more truthfully and accurately.
Owner:ZHEJIANG CELLPRO BIOTECH
Electroporation method for transfection of mammal embryo with siRNA (small interfering Ribose Nucleic Acid)
InactiveCN102329819AIncreased mortalityStrong penetrating powerFermentationGenetic engineeringCell membraneRoom temperature
The invention discloses an electroporation method for transfection of mammal embryo with siRNA (small interfering Ribose Nucleic Acid). The electroporation method comprises the following steps: (1) weakening an embryo zona pellucid; (2) preparing electroporation solution containing siRNA; and (3) conveying the embryo into the prepared electroporation solution containing siRNA, standing at the room temperature, and then conveying the embryo and the electroporation solution into an electric turn trough for electroporation. The electroporation method comprises the steps of weakening the embryo zona pellucid and complexing the siRNA, and then placing the embryo under the action of a pulsed electric field to increase the permeability and the membrane conductance of the embryo instantaneously, and thus the siRNA which is difficult to penetrate through a cell membrane under the normal physiological condition enters the cell. Statistic observation and analysis prove that the stable positive transfection efficiency of survival embryos exceeds 95%, and the practical effective utilization rate of the collected total embryo exceeds 70%. The electroporation method is a safe, simple and efficient transfection technology of mammal embryo with siRNA.
Owner:NORTHWEST A & F UNIV +1
Protein chip for female infertility detection and kit thereof
The present invention discloses a dedicated multi-index protein chip for female infertility detection and a kit thereof. The protein chip consists of a substrate, protein detection indexes arranged on the substrate in an array mode and a contrast coating for the detection indexes. The protein detection indexes cover ovary antigen (OAg), endometrium antigen (EAg), sperm antigen (SAg), zona pellucida antigen (ZAg), human chorionic gonadotropin antigen (HCG), trophoblast antigen (TAg) and cardiolipin antigen (CAg). The present invention overcomes the defects of the existing autoimmunological detection products on market, such as single detection function, lagged detection indexes and no integration. Besides the indexes of the product (patent application No: 200510102466.3), the protein chip also has the ACA index. The density of all indexes can be quantitatively detected, and the cross reaction of all indexes is eliminated. The present invention not only has high specificity and high sensitivity, but also has high accuracy and precision.
Owner:上海裕隆生物科技有限公司
Sterility, infertility six-index integral investigating reaction plate and protein chip kit
The invention relates to an integrated detection reaction orifice plate and protein chip reagent box for detecting six sterility indexes. And the reaction orifice plate comprises a substrate and reaction orifices on the substrate and the reaction orifices comprise 3-384 sample orifices, 1-300 negative contrast orifices, and 1-300 positive quality control orifices, where at the bottom of each reaction hole is solid carrier, coated with micro lattice of Sperm antigen (SAg), Zona pellucida antigen (ZPAg), Endometrium antigen (EMAg), Ovary antigen (OAg), Trophoblast antigen (TAg), and Human Chorionic Gonadotropin antigen (HCGAg) antigens or more. And the reaction orifice plate and reagent can simply and conveniently, rapidly and accurately implement simultaneously detection of six sterility indexes for many persons.
Owner:汪宁梅
Microfluidic channel embryo and/or oocyte handling, analysis and biological evaluation
InactiveCN1441652AEliminate processingEasy to collectBioreactor/fermenter combinationsBiological substance pretreatmentsChannel geometrySurvivability
Microfluidic embryo scaled channels (14) for handling and positioning embryos provide the opportunity to evaluate and treat embryos in improved manners. Fluid flow is used to move and position embryos within microfluidic channels and channel geometrics may be used to place embryos at specific locations. Surface properties and compliance (deformation) properties of embryos are evaluated as a predictor of viability. The microfluidic channels provide the opportunity for fine controls of pressure to conduct various evalutions at forces slightly below which damage to embryos is known to occur. Measurement of the distance and / or which embryos roll in a same pressure gradient microfluidic channel provides information, with healthy embryos traveling slower or a shorter distance as they demonstrate more stiction to channel walls. Positioned at a constriction (14a, 14b, 24, 26), health embryos also appear to deform less than unhealthy embryos that are more readily pulled into a constriction. In addition, healthy embryos appear to resume their shape better. Fluid from microfluidic channels is easily collected downstream without altering the embryo environment, providing a better opportunity for chemical analysis of fluid chemical analysis than convention manual handling and sampling techniques. Zona pellucida removal of mammalian embryos is achieved as embryos are moved through flow to a precise location where lysing agent can be washed over the embryo to achieve zona removal. Cumulus removal is realized with a series of constrictions to cut cumulus followed by fluid flows to remove cut cumulus from the embryo.
Owner:THE BOARD OF TRUSTEES OF THE UNIV OF ILLINOIS
Vibration type microinjection device
ActiveUS20090130743A1Automatic controlImprove accuracyBioreactor/fermenter combinationsBiological substance pretreatmentsElectricityNuclear membrane
A vibration type microinjection device capable of ensuring smooth piercing of membranes having different properties such as a zona pellucida, a cell membrane and a nuclear membrane included in a fertilized egg with high accuracy and efficiency is provided.A vibration type microinjection device comprises a vibrator (28) which is connected in series with a micropipette (8) and which has a piezoelectric actuator (29) installed in a housing, and a signal control device (21) for controlling an electric signal applied to the piezoelectric actuator (29), wherein vibration is applied in the longitudinal direction of the micropipette (8) via the vibrator (28) by inputting an electric signal to the piezoelectric actuator (29). By such configuration, smooth piercing of membranes having different properties such as a zona pellucida, a cell membrane and nuclear membrane included in a fertilized egg is realized with high accuracy and efficiency.
Owner:MIYAWAKI FUJIO
Serum-free separating and culturing method for sheep embryo stem cell
InactiveCN101914487ABroaden the field of studySignificant technological progressEmbryonic cellsGerm cellsSerum freeStem cell culture
The invention provides a serum-free separating and culturing method for a sheep embryo stem cell, comprising the following steps of: removing a zona pellucida of sheep blastula cultured in vitro with a Tyrode's Solution and then inoculating the sheep blastula to a serum-free culture solution of the sheep embryo stem cell and fixing by using a needle head; placing under the conditions that the temperature is 38.6DEG C and the saturated humidity is 5 percent CO2 for culturing; during the culturing, replacing the culture solution in a half quantity every 48 hours with the pH value of 6.8-7.2 and primarily culturing for 7-9 days; and carrying out the transfer culture once according to the ratio of 1:2-1:4 by adopting a conventional mechanical method, that is to say, transferring the sheep embryo stem cell cultured in vitro to the 16th generation. The serum-free culture solution of the sheep embryo stem cell is prepared from D-MEM / F-12+80ng / mL bFGF+3muMCHIR 99021+10mu L / mL N2+20mu L / mL B27+10mu L / mL NEAA+2mM L-Glutamine+0.2mM beta-Mercaptoethanol. Bared embryos are fixed at the bottom of a culture dish by using a No.32 needle head so as to avoid the damage to the cells in the blastula, and a baked embryo trophocyte is stripped off. The preferable inoculating amount of the baked embryos is 45-60 / groups. The method can ensure that the formation rate of primary colony of sheep embryo stem cells is improved to 33 percent (14 / 42).
Owner:新疆维吾尔自治区畜牧科学院中国-澳大利亚绵羊育种研究中心
Animal model with male reproductive disorders, as well as preparation method and application thereof
InactiveCN103361336ADoes not affect normal developmentDoes not affect hormone levelsMicrobiological testing/measurementRecombinant DNA-technologyUterusMature sperm
The invention provides an animal model with male reproductive disorders of a non-human mammalian animal, as well as a preparation method and application of the animal model, and further identifies the functions of a Prss37 gene and a protein. The Prss37 gene of the animal model is inactivated, thereby affecting the appearance of ADAM3 in mature sperms produced by the animal model, indirectly affecting the combination of the sperms-zona pellucida and the migration of the sperms from the uterus to the oviduct and resulting in the serious male reproductive disorders. The model, the Prss37 gene and the protein thereof can be used for screening ADAM3 ripening accelerators, as well as sterility infertility treatment agents or contraceptive medicaments.
Owner:上海南方模式生物研究中心 +1
Trophectodermal Cell-Specific Gene Transfer Methods
InactiveUS20090013418A1Easy to operateVirusesGenetically modified cellsGerm layerHereditary Mutation
The present inventors discovered that genes could be introduced specifically into trophectodermal cells with high efficiency, by infecting blastocysts with viral vectors carrying an arbitrary polynucleotide, or by using a nucleic acid transfection reagent in blastocysts, from which zona pellucida (extracellular matrix covering preimplantation early embryos to protect them from infection of viruses and the like) is removed. This method has no risk of infecting cells of the inner cell mass, which develops into a fetus in the future, with the introduced polynucleotide because the trophectoderm serves as a barrier. The present invention provides methods for introducing foreign genes into only placenta but not fetus, which enables rescue of genetically mutant animals from embryonic lethality due to placental abnormality and allows their birth. Furthermore, it is possible to analyze expression and effect of genes that regulate placental formation or placental function by using these methods.
Owner:OKABE MASARU +2
Method for evaluating cell quality
InactiveCN104531827AReliable evaluationImprove efficiencyMicrobiological testing/measurementCell engineeringComputer science
The invention belongs to the technical field of cell engineering, and particularly relates to a method for evaluating cell quality. The method comprises the following steps: A, acquiring useful information of morphological characters of an object cell; B, acquiring the elasticity modulus of zona pellucida of the object cell; C acquiring a bio-electrical signal fed back by the object cell; D, comprehensively evaluating the object cell according to the performed steps. The method for evaluating the cell quality by adopting the technical scheme can be used for effectively reliably evaluating the cell quality with high throughput and high efficiency.
Owner:XIAMEN UNIV
Method for producing somatic cell cloned bovine blastocyst
InactiveCN102766655AImprove fusion rateIncrease cleavage rateGenetic engineeringFermentationBovine oocyteCells oocytes
he invention discloses a method for producing somatic cell cloned bovine blastocysts. According to the method, to bovine ear limbal fibroblasts are used as nuclear transfer donorcells, and mature bovine oocytes cultivated in vitro are used as nuclear transfer receptor cells; the oocytes are denucleated by extrusion and removed with the zona pellucida; the oocytes and the donor cells are adhered in an arrangement way of oocytes-donor cells-oocytes and treated with electro-fusion to obtain reconstructed embryos; and the reconstructed embryos are activated and cultured in vitro to obtain the cloned blastocysts. The method can improve the production efficiency of the cloned bovine blastocysts.
Owner:INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
Epitope minimum motif peptide of human zona pellucida protein
The invention belongs to the biological engineering and immunology technology fields and specifically relates to a linear epitope minimum motif peptide of human zona pellucida (huZP) protein huZP1 and huZP3, and ZP chimeric peptides. In particular, the invention provides the minimum epitope amino acid sequence on the huZP1 and the huZP3 proteins for detecting ZP antibodies (Leading to the sterility or premature ovarian failure of women) and the 8-peptide sequence containing the epitope minimum motif and of truncated GST188 carrier protein fusion expression. The epitope minimum motif peptide of the huZP1 with immunogenicity can be identified by a rabbit anti-pig ZP antiserum and the 5-epitope minimum peptide motif of huZP3 can be identified by a rabbit antibody human ZP3 antiserum, to prove the value of the epitope minimum motif peptide when used as a single-epitope or multi-epitope peptide contraception antigen or when used as a single-epitope or multi-epitope fusion expression antigen for detecting the ZP antibodies of humans.
Owner:SHANGHAI INST OF PLANNED PARENTHOOD RES +2
Human zona pellucida proteins and methods of their use in diagnosing male infertility
InactiveUS7148021B2Cell receptors/surface-antigens/surface-determinantsPeptide/protein ingredientsPhysiologyDiagnosis laboratory
Reagents, methods, and kits are described that are useful for fertility testing and that in many cases provides much faster, convenient and rapid determination of male infertility. The reagents described include properly glycosylated human ZP3, ZP2, ZP1, glycosylated peptides thereof, fusion proteins such as green fluorescent protein—ZP3, non-covalent complexes of ZP2 with ZP3, fusion protein of ZP2–ZP3, solid phase materials such as agarose beads coated with binding agents such as ZP3, and other artificial zona. Methods are provided that, in many cases convert a complex biological event into a well defined biochemical binding event based on one or more of the reagents. Such methods are much easier to set up and monitor, allowing more convenient and inexpensive diagnostic testing for male fertility. The acrosome reaction is detected in other embodiments by virtue of quantitating one or more released substances. Kits are further provided that contain one or more reagents useful for testing at a diagnostic laboratory or other facility.
Owner:TRINITY BIOMEDICAL TECH CORP +1
Method for detecting chromosome abnormality of embryos by aid of blastocyst cultivation solution without zona pellucida
The invention provides a method for detecting chromosome abnormality of embryos by the aid of blastocyst cultivation solution without zona pellucida. The method which is particularly an in-vitro non-therapeutic method for detecting the chromosome abnormality of the embryos by the aid of the blastocyst cultivation solution includes steps of (a), providing the cultivation solution from blastocyst cultivation systems; (b), carrying out gene detection on the cultivation solution so as to authenticate whether chromosomes of the embryos are abnormal or not. The zona pellucida of the embryos in the blastocyst cultivation systems is removed before the embryos are cultivated, the embryos are cultivated in the blastocyst systems for 3-6 days, and preferably, the cultivation solution is separated from the cultivation systems after 4 days. The method has the advantages that contamination and interference risks due to redundant sperms and parent-source granular cells in IVF (in-vitro fertilization) and ICSI (intracytoplasmic sperm injection) technical procedures can be eliminated, and whether the chromosomes of the embryos are abnormal or not can be accurately authenticated.
Owner:XUKANG MEDICAL SCI & TECH (SUZHOU) CO LTD
Human oocyte zona pellucida protein multi-epitope chimeric peptide antigen and preparation method thereof
InactiveCN101696239AMicroorganism based processesAntibody medical ingredientsEscherichia coliPeptide antigen
The invention belongs to the technical field of immunology and bioengineering and particularly relates to human oocyte zona pellucida protein multi-epitope chimeric peptide (ZPCP) and a preparation method and an application thereof. A ZPCP molecule designed by the invention is combined with three proteins of huZP (huZP1, huZP2 and huZP3), nine linear B cell epitopes (BCE) and four wide-adaptability helper T cell epitopes (TCE) and comprises a section of pig ZP4 (pZP4), N terminal 25 peptide and a beta turn forming peptide (GPSL). The ZPCP molecule has the advantages that an amino acid residue sequence of the ZPCP molecule or a DNA coding sequence thereof can be highly expressed in a escherichia coli; a targeting expression protein with the electrophoresis uniformity higher than 95 percent can be conveniently obtained; the ZPCP molecule can generate a high level anti ZPCP antibody in an induction way in a rabbit body as an immunogen, including anti each embedded BCE antibody which can be detected in serum.
Owner:SHANGHAI INST OF PLANNED PARENTHOOD RES +2
Pharmaceutical composition for treating or preventing ovarian cancer
The invention relates to therapeutic and prophylactic treatment of ovarian cancer and metastases thereof. More specifically, the invention relates to immunogenic polypeptides comprising at least a portion of an ovarian tissue cell-associated protein or immunologically active variants thereof and to nucleic acids encoding such polypeptides and to the use thereof in immunotherapeutic methods of treatment. Said immunogenic polypeptides are provided by the zona pellucida (ZP) glycoproteins. ZP glycoproteins and fragments thereof that can induce a CD8+ and / or CD4+ T cell response as well as nucleic acid sequences encoding them can suitably be used in the present immunotherapeutic strategies.
Owner:PANTARHEI BIOSCI
Enhancement of mammalian embryo development
InactiveUS20060160213A1Improved implantPromote embryonic developmentOrganic active ingredientsArtificial cell constructsMammalProstaglandin analog
A method of enhancing in vitro development of a mammalian embryo is disclosed which comprises supplementing the culture medium with a prostaglandin, or a prostaglandin analog, in an amount effective to promote complete hatching of the embryo (i.e., freeing of the embryo from the zona pellucida). The quality of human blastocysts is enhanced in vitro by culturing with a prostacyclin agonist, Iloprost. The in vivo implantation potential and live birth potential of an in vitro fertilization embryo is thereby enhanced and establishment of a viable pregnancy is facilitated.
Owner:BOARD OF RGT THE UNIV OF TEXAS SYST
Cell nuclear transfer
The present invention discloses methods for cell nuclear transfer that comprise for example modification of zona pellucida of an oocyte, and / or sectioning of oocytes into several parts. The present invention also discloses methods for producing a genetically modified non-human mammal. Genetically modified non-human mammals obtainable by the disclosed methods are also within the scope of the present invention. Disclosed are also methods for cryopreservation of cells.
Owner:AARHUS UNIV
Isolation of Inner Cell Mass for the Establishment of Human Embryonic Stem Cell (hESC) Lines
InactiveUS20070048864A2Preventing possibility of transmissionSafely be commercial scaleArtificial cell constructsMammal material medical ingredientsGerm layerStem cell line
A method for isolating an inner cell mass comprising the steps of immobilizing a blastocyst stage embryo having a zona pellucida, trophectoderm, and inner cell mass, creating an aperture in the blastocyst stage embryo by laser ablation, and removing the inner cell mass from the blastocyst stage embryo through the aperture. The aperture is through the zona pellucida and the trophectoderm. The laser ablation is acheived using a non-contact diode laser. The inner cell mass removed from the blastocyst stage embryo is used to establish human Embryonic Stem Cell lines.
Owner:RELIANCE LIFE SCI PVT
Special culture medium and method for culturing porcine trophoderm stem cells
ActiveCN104946581AAvoid pollutionImprove the safety of useEmbryonic cellsGerm cellsHeterologousCell-Extracellular Matrix
The invention discloses a special culture medium and method for culturing porcine trophoderm stem cells. The special culture medium is composed of bFGF, activin A, Y27632, Knockout SR and beta-mercaptoethanol. The culture method comprises the following steps: previously coating a stem cell culture dish with an extracellular matrix; digesting porcine blastula with proteinase to remove the zona pellucida, transferring into the stem cell culture dish, and adding the special culture medium; and culturing until a trophoderm stem cell cloning group with the total cell count of 1000-2000 is formed, digesting into unicells, transferring into a new extracellular-matrix-coated culture dish, and carrying out cell subculture. The special culture medium has the advantages of definite components and high use safety, and avoids the pollution of heterogenous cells. The culture method is simple and efficient, and has the advantages of high cloning formation rate, low apoptosis rate, high proliferation and subculture capacity, high safety and high stability when being used for porcine trophoderm stem cell culture.
Owner:WENS FOOD GRP CO LTD +1
Method for removing mouse oocyte nuclei by adopting zona pellucida solution cavity method
The invention discloses a method for removing mouse oocyte nuclei by adopting a zona pellucida solution cavity method. The method comprises the following steps of: performing central slight dripping and peripheral slight dripping in a plastic utensil; making an acid Tyrode's solution react with a zona pellucida under a microscope; forming a pore on the zona pellucida 1-6 seconds later by dissolving till the zona pellucida becomes thin and soft and cytoplasm protrudes outwards; absorbing a first polar body and surrounding cytoplasm out; releasing an oocyte; and denucleating and observing integrality by adopting trypan blue dyeing, wherein the denucleation success rate is over 99.1 percent. In the preparation method, used equipment is simple and the denucleation operation can be completed under a micromanipulator; a reagent has the advantages of simple components, easiness of preparation, low cost, low difficulty of operation, easiness of comprehension, soft denucleation operation, small mechanical damage, quick denucleation and high efficiency; the average denucleation time of each oocyte is 1-6 seconds, the survival rate of each denucleated oocyte is over 97 percent and the success rate of denucleation is over 99 percent; and the method is suitable for researching mouse nucleus transplantation in a Piezo-free laboratory and early events relevant to embryonic development.
Owner:HENAN UNIV OF SCI & TECH
Piezoelectric ultrasonic cutting system and method for oocyte zona pellucida
ActiveCN108456641AAvoid damagePlay an auxiliary supporting roleBioreactor/fermenter combinationsBiological substance pretreatmentsSurface layerEngineering
The invention discloses a piezoelectric ultrasonic cutting system and method for oocyte zona pellucida. The piezoelectric ultrasonic cutting system comprises an oocyte posture adjusting chip, an oocyte holding device and an oocyte ultrasonic cutting scalpel, wherein the oocyte posture adjusting chip is used for performing posture adjustment and bottom fixation and holding on oocytes; the oocyte holding device comprises a bent holding micro-needle and a micro-needle clamp used for clamping the bent holding micro-needle; the end of the bent holding micro-needle is provided with a single-side inwards recessed arc holding needle head; a bayonet is formed inside the bent holding micro-needle, and a notch is formed in the end of the bent holding micro-needle; the oocyte ultrasonic cutting scalpel is used for cutting the oocyte zona pellucida from the notch position. According to the piezoelectric ultrasonic cutting system disclosed by the invention, the defect that the oocytes are damaged due to longitudinal conduction of ultrasonic energy can be effectively overcome, the zona pellucida is cut from a tangential direction of the oocyte surface layer by the oocyte ultrasonic cutting scalpel, and damage of the oocytes can be reduced.
Owner:SUZHOU UNIV
External detection method for sperm-oocyte interaction
InactiveCN101482555AReduce consumptionImprove accuracyBiological testingFluorescence/phosphorescenceFluoresceinAcrosome reaction
The invention provides a sperm-oocyte interaction vitro detection method, comprising: a. extracting sperms by a swim-up method or density gradient method; b. mixing the sperms and oocyte extracted from the step a according a certain proportion in the culture medium and setting a blank control of the oocyte in the culture medium; c. removing the sperms which are bonded with the surface of the oocyte in the step b and detecting the number of sperms bonded with the transparent area of the oocyte under an inverted phase contrast microscope; d. separating the oocyte from the sperms bonded with the transparent area of the oocyte and respectively collecting the separated sperms and the oocyte; e. coating the sperms in the blank control of the oocyte and sperms separated by the step b on the glass slide, drying and fixing the sperms using the fixation fluid, cleaning the fixed sperms and adding fluorescein-agglutinin label, re-cleaning the sperms after reaction, detecting the sperm acrosome reaction rate under a fluorescence microscope; f. detecting the number of sperms penetrating into the oocyte separated by the step d under the inverted phase contrast microscope. The detection method reduces the consumption of the label resource and the detection cost and time and increases the detection efficiency.
Owner:刘瑜
Method for enucleating mammal oocyte
ActiveCN109321518AShow exactly where you areReduce exposure timeFermentationGenetic engineeringFluorescenceCytoplasm
The invention belongs to the technical field of embryo engineering, and particularly relates to a method for enucleating mammal oocyte. The method comprises the following steps: obtaining cumulus oocyte compositelex; removing cumulus granulose cells at the periphery of an oocyte transparent zone in the cumulus oocyte compositelex, and leaving oocyte; incubating the obtained oocyte to nucleate; putting the nucleated oocyte into culture solution liquor to treat; debugging a fluorescent lamp; cleaning the oocyte treated by the culture solutionliquor, and transferring the cleaned oocyte into enucleatingstoning liquid drops; and enucleating under fluorescence. The method is simple and convenient to operate, greatly reduces loss of cytoplasm, ensures that each constructed clone embryo loses a little cytoplasm and achieves 100% enucleating, and improves developmental potentiality of the embryos.
Owner:WENS FOODSTUFF GRP CO LTD
Method for slaking oocyte transparent tape
The present invention relates to method of digesting the zona pellucida of oocyte. The method is to set oocyte inside glutathione solution for digesting. The method of digesting the zona pellucida of oocyte in glutathione solution has simple operation, short digesting period, less damage to cell and low cost.
Owner:CHINA AGRI UNIV
Who we serve
- R&D Engineer
- R&D Manager
- IP Professional
Why Patsnap Eureka
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com