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Method for evaluating cell quality

A quality evaluation and cell technology, applied in the field of cell engineering, can solve the problem that cells cannot be measured in real time, and achieve the effect of reliable evaluation

Inactive Publication Date: 2015-04-22
XIAMEN UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Most of the existing methods and device systems study a certain aspect separately, and then use other experimental methods to analyze the results, which cannot be measured in real time for cells.

Method used

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  • Method for evaluating cell quality

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Embodiment Construction

[0020] The present invention will be further described in conjunction with the accompanying drawings and specific embodiments.

[0021] The present invention proposes a method for evaluating cell quality, comprising the steps of:

[0022] A, the step of obtaining useful information on the morphological characteristics of the subject cells; specifically including:

[0023] A1, to obtain the morphological images of the target cells through the micro-object optical photography system;

[0024] A2. Use the image processing system to obtain the useful characteristic parameter information of the morphological characteristics of the above-mentioned morphological image, such as the parameter information of the shape geometry of the target cells (such as embryonic cells), the parameter information of the color, the parameter information of the degree of lightness, and the blastomere. The parameter information of size uniformity (consistency), the parameter information of cytoplasmic g...

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Abstract

The invention belongs to the technical field of cell engineering, and particularly relates to a method for evaluating cell quality. The method comprises the following steps: A, acquiring useful information of morphological characters of an object cell; B, acquiring the elasticity modulus of zona pellucida of the object cell; C acquiring a bio-electrical signal fed back by the object cell; D, comprehensively evaluating the object cell according to the performed steps. The method for evaluating the cell quality by adopting the technical scheme can be used for effectively reliably evaluating the cell quality with high throughput and high efficiency.

Description

technical field [0001] The invention belongs to the technical field of cell engineering, in particular to an evaluation method of cell quality, specifically a method for obtaining a cell quality evaluation system through comprehensive evaluation based on detected cell morphology, elastic modulus, and membrane potential. Background technique [0002] With the development of assisted reproductive technology (ART), the application of in vitro fertilization (in vitro fertilization, IVF), embryo transfer and its derivative technologies has improved the conception and pregnancy rate, but at the same time, the multiple embryo pregnancy rate and its The various complications brought about have also been significantly improved. In order to avoid multiple embryo pregnancy and multiple embryos, the Ministry of Health of China stipulates that women aged ≤35 years old should not transfer more than 2 embryos in the first cycle of IVF; for multiple embryo pregnancy, fetal reduction surgery...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02
Inventor 谢瑜刘翀曾凤
Owner XIAMEN UNIV
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