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Sterility, infertility six-index integral investigating reaction plate and protein chip kit

An integrated detection, infertility technology, applied in the field of in vitro clinical tests and biochips, can solve the problems of labor-intensive, high-cost, time-consuming, etc.

Inactive Publication Date: 2006-11-29
汪宁梅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, the serological detection of infertility is the indirect method of immunoenzyme labeling (ELISA), which can detect the above-mentioned antibodies related to infertility in the human body. These antibodies exist in the blood of the human body or in parts of the human reproductive tract, such as In seminal plasma and cervical mucus, but ELISA reagents can only detect a single indicator at a time, which is time-consuming, labor-intensive, and costly for multi-indicator detection, and it is difficult to meet the clinical diagnosis market with large samples

Method used

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  • Sterility, infertility six-index integral investigating reaction plate and protein chip kit
  • Sterility, infertility six-index integral investigating reaction plate and protein chip kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Preparation of detection reaction plate

[0040] Take a 96-well plate and set 42-84 sample wells, 1-8 negative control wells, and 1-8 positive quality control wells. A solid phase carrier (NC membrane or PDVF membrane) is placed at the bottom of each reaction well, and the solid phase carrier is coated with sperm antigen (SAg), zona pellucida antigen (ZPAg), endometrial antigen (EMAg), Ovarian antigen (OAg), trophoblast antigen (TAg), human chorionic gonadotropin antigen (HCGAg) protein microarray of six antigens ( figure 1 ).

Embodiment 2

[0042] kit preparation

[0043] Prepare a test kit containing six indicators of infertility integrated detection, which contains a reaction well plate (8-200 people) of embodiment 1, 1 bottle of washing concentrate, 1 bottle of enzyme-labeled working solution, and detection solution A One bottle, one bottle of detection solution B, one bottle of positive control, and one bottle of negative control.

[0044] Washing Concentrate, Enzyme Label Working Solution, Detection Solution A, Detection Solution B

Embodiment 3

[0046] Integrated detection of six indicators of infertility

[0047] Test each blood sample according to the following method:

[0048] 1. Add diluted blood to be tested or negative control, positive control substance in the corresponding reaction well, 60-200ul / well.

[0049] 2. 30°C-42°C heat preservation and shaking reaction for 30-60 minutes.

[0050] 3. Wash 4-30 times with lotion, 1-10 minutes each time, vibrate.

[0051] 4. Add enzyme-labeled working solution 50-100ul to each well.

[0052] 5. Wash 4-6 times with lotion, 1-10 minutes each time, vibrate.

[0053] 6. Add detection solution 20-100ul / well, so that the detection solution is evenly distributed at the bottom of the well.

[0054] 7. CCD detection, exposure for 3-15 seconds depending on the intensity of the luminescent signal.

[0055] Result judgment:

[0056] According to the CutOff value of different indicators, refer to the negative control and positive quality control blank to determine the negative...

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Abstract

The invention relates to an integrated detection reaction orifice plate and protein chip reagent box for detecting six sterility indexes. And the reaction orifice plate comprises a substrate and reaction orifices on the substrate and the reaction orifices comprise 3-384 sample orifices, 1-300 negative contrast orifices, and 1-300 positive quality control orifices, where at the bottom of each reaction hole is solid carrier, coated with micro lattice of Sperm antigen (SAg), Zona pellucida antigen (ZPAg), Endometrium antigen (EMAg), Ovary antigen (OAg), Trophoblast antigen (TAg), and Human Chorionic Gonadotropin antigen (HCGAg) antigens or more. And the reaction orifice plate and reagent can simply and conveniently, rapidly and accurately implement simultaneously detection of six sterility indexes for many persons.

Description

technical field [0001] The invention belongs to the field of in vitro clinical testing and biochip technology. Specifically, it is a method suitable for six indicators of infertility: sperm antibody (ASAb), anti-zona pellucida antibody (AZP), and anti-endometrium antibody (EMAb). ), anti-ovarian antibody (AOA), anti-trophoblast antibody (ATAb), anti-human chorionic gonadotropin antibody (HCGAb) integrated detection reaction plate and kit. Background technique [0002] According to the prediction of the World Health Organization, in the 21st century, infertility will become the third largest disease after tumors and cardiovascular and cerebrovascular diseases. Authoritative data show that the incidence of unwanted infertility among married couples is 7% to 46%. Although there are differences in different regions, it is generally considered to be between 8% and 15%. [0003] With the development of medical science and the continuous improvement of detection methods, people p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/547G01N33/52
Inventor 汪宁梅
Owner 汪宁梅
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