290 results about "Nuclear material" patented technology

This invention provides a method for deriving precursors to pluripotent non-embryonic stem (P-PNES) and pluripotent non-embryonic stem (PNES) cell lines. The present invention involves nuclear transfer of genetic material from a somatic cell into an enucleated, zona pellucida free human ooplastoid having a reduced amount of total cytoplasm. The present invention provides a new source for obtaining human and other animal pluripotent stem cells. The source utilizes as starting materials an oocyte and a somatic cell as the starting materials but does not require the use, creation and/or destruction of embryos or fetal tissue and does not in any way involve creating a cloned being. The oocyte never becomes fertilized and never develops into an embryo. Rather, portions of the oocyte cytoplasm are extracted and combined with the nuclear material of individual mature somatic cells in a manner that precludes embryo formation. Murine, bovine, and human examples of the procedure are demonstrated. Subsequently, the newly constructed P-PNES cells are cultured in vitro and give rise to PNES cells and cell colonies. Methods are described for culturing the P-PNES cells to yield purified PNES cells which have the ability to differentiate into cells derived from mesoderm, endoderm, and ectoderm germ layers. Methods are described for maintaining and proliferating PNES cells in culture in an undifferentiated state. Methods and results are described for analysis and validation of pluripotency of PNES cells including cell morphology, cell surface markers, pluripotent tumor development in SCID mouse, karyotyping, immortality in in vitro culture.

The invention provides a multifunctional nuclear shell structure drug carrier material and a preparation method thereof; the preparation method comprises the following steps: step one, adopting a solvent-thermal method for preparing monodispersed ferroferric oxide magnetic nanoparticles with grain diameter of about 60nm as ferromagnetic nuclear material of the nuclear shell structure; step two, adopting a sol-gel method for cladding an imporous silicon dioxide layer and a meso-porous layer outside ferromagnetic nucleus in sequence; step three, adopting the sol-gel method for loading a layer ofup-conversion fluorescent material NaYF4: Yb, Er on the material obtained in the step two, wherein the molar concentration of Yb occupies 17% of Y concentration, and the molar concentration of Er occupies 3% of Y concentration. In the invention, an inertia SiO2 layer is designed between the magnetic nucleus and post-functionalized rare earth luminescent material for separating magnetic material from a rare earth luminescent layer so as to prevent fluorescent quenching; up-conversion fluorescent powder with higher fluorescent efficiency is used as fluorescent material; and the sol-gel method with mild reaction condition and uniform dispersion is adopted for forming the nuclear shell structure.

Propulsion from combustion of solid propellant pellet-projectiles for providing a useful propulsion that has the advantages of both solid and liquid propulsion engines, and also can make use of either solid chemical propellants or fissionable nuclear material as the fuel. Preferred methods and systems can include a storage chamber for storing solid propellant pellets, a feeding system having a pellet feeding channel and a pellet feeding mechanism connected to the storage chamber and to a gun assembly, which is positioned along a longitudinal axis to eject the pellets at a certain velocity. A triggering system positioned between gun assembly and thrust chamber can initiate the propellant pellet-projectile, and a thrust chamber having a combustion chamber for combustion of propellant pellet-projectile with an exhaust nozzle. Additionally, an auxiliary power system can be used to power the components and various electrical and electronic systems that may be present in the invention for controlling the engine components. The gun assembly may include an ejector mechanism for ejecting the propellant pellets through at least one barrel. The triggering system can produce a medium creating an ambience for the initiation of propellant pellet-projectiles. Methods and systems can be used for space and rocket crafts, turbojets and ramjets.