Epitope minimal motif peptides of human zona pellucida protein-4 and extended short peptides and application thereof

A technology of zona pellucida protein and epitope, which is applied in the fields of bioengineering and immunology, and can solve problems such as the preventive or therapeutic effect of synthetic peptide vaccines with limited number of combined epitopes

Inactive Publication Date: 2012-09-12
SHANGHAI INST OF PLANNED PARENTHOOD RES +1
View PDF2 Cites 19 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as far as a single target antigen is concerned, the above studies are limited by the number of identified linear epitopes in the past. Due to the limited n...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Epitope minimal motif peptides of human zona pellucida protein-4 and extended short peptides and application thereof
  • Epitope minimal motif peptides of human zona pellucida protein-4 and extended short peptides and application thereof
  • Epitope minimal motif peptides of human zona pellucida protein-4 and extended short peptides and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Fine epitope identification of mouse anti-human ZP4 monoclonal antibody MA-1671

[0021] Materials and methods:

[0022] 1. The human ZP4 gene clone was provided by the Laboratory of Cell and Developmental Biology, National Institutes of Health. Heat-inducible expression plasmids pSY621 and pXXGST-1 were constructed by the inventors of this patent [Shen Yun, Ying Kang, Xu Wanxiang, Xie Yi: Construction of high-efficiency expression vector pSY621 in Escherichia coli. Fudan Journal ( Natural Science Edition ) 2000; 39(3):313–317; Xu WX, et al. Minimal motif mapping of a known epitope on human zona pellucida protein-4 using a peptide biosynthesis strategy. J Reprod Immunol 2009; 81:9-16]. Escherichia coli BL21 (DE3) strain was preserved by the State Key Laboratory of Genetic Engineering, Fudan University.

[0023] 2. Mouse anti-huZP4 monoclonal antibodies MA-1662 and MA-1671 were prepared by the inventors (Bukovsky A, Gupta SK, et al. Production of monoclon...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of biomedicine and biological detection, in particular to two linear epitope minimal motif peptides capable of being identified by human zona pellucida protein-4 (ZP4) monoclonal antibodies MA-1662 and MA-1671 on human ZP4 and extended eight peptides and application thereof. The invention provides two candidate epitopes which can be used for developing human immune birth control recombinant multi-epitope vaccines, and the amino acid sequences of the candidate epitopes are shown as SEQ ID No. 1 and marked as huZP4147-151 or shown as SEQ ID No.2 and marked as huZP4256-260; and the invention also provides candidate epitopes which can be used for developing novel high-specificity high-sensitivity recombinant multi-epitope detection antigens for diagnosing serum ZP antibodies of ZP autoimmune infertile women, and the amino acid sequences of the candidate epitopes are shown as SEQ ID No.3-SEQ ID No.6.

Description

technical field [0001] The invention belongs to the technical fields of bioengineering and immunology, and specifically relates to an epitope minimal motif peptide of human egg zona pellucida protein-4 that can be recognized by a specific monoclonal antibody, an extended short peptide thereof and applications thereof. Background technique [0002] Except for mice, the zona pellcida (ZP) of mammals including humans is composed of four glycoproteins (named ZP1, ZP2, ZP3 and ZP4, respectively). As one of the anti-fertility links, blocking the fertilization of human sperm and eggs to cause infertility is undoubtedly the most ideal way that will not cause controversy. Therefore, many laboratories in the world have been conducting research on ZP contraceptive vaccines for more than 40 years. Although many studies using mice and rabbits as animal models have confirmed the sufficient effectiveness of active immunization with ZP or a single ZP3 protein antigen for anti-fertility, al...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07K7/06A61K39/00A61P15/18G01N33/68
Inventor 徐万祥顾少华赛提旭·古普塔贝纳·布罕达瑞山奇塔·乔德哈瑞王健季朝能谢毅唐海平
Owner SHANGHAI INST OF PLANNED PARENTHOOD RES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap