Epitope minimal motif peptides of human zona pellucida protein-4 and extended short peptides and application thereof

Abstract

The invention belongs to the technical field of biomedicine and biological detection, in particular to two linear epitope minimal motif peptides capable of being identified by human zona pellucida protein-4 (ZP4) monoclonal antibodies MA-1662 and MA-1671 on human ZP4 and extended eight peptides and application thereof. The invention provides two candidate epitopes which can be used for developing human immune birth control recombinant multi-epitope vaccines, and the amino acid sequences of the candidate epitopes are shown as SEQ ID No. 1 and marked as huZP4147-151 or shown as SEQ ID No.2 and marked as huZP4256-260; and the invention also provides candidate epitopes which can be used for developing novel high-specificity high-sensitivity recombinant multi-epitope detection antigens for diagnosing serum ZP antibodies of ZP autoimmune infertile women, and the amino acid sequences of the candidate epitopes are shown as SEQ ID No.3-SEQ ID No.6.

Description

technical field [0001] The invention belongs to the technical fields of bioengineering and immunology, and specifically relates to an epitope minimal motif peptide of human egg zona pellucida protein-4 that can be recognized by a specific monoclonal antibody, an extended short peptide thereof and applications thereof. Background technique [0002] Except for mice, the zona pellcida (ZP) of mammals including humans is composed of four glycoproteins (named ZP1, ZP2, ZP3 and ZP4, respectively). As one of the anti-fertility links, blocking the fertilization of human sperm and eggs to cause infertility is undoubtedly the most ideal way that will not cause controversy. Therefore, many laboratories in the world have been conducting research on ZP contraceptive vaccines for more than 40 years. Although many studies using mice and rabbits as animal models have confirmed the sufficient effectiveness of active immunization with ZP or a single ZP3 protein antigen for anti-fertility, al...

AI Technical Summary

Problems solved by technology

However, as far as a single target antigen is concerned, the above studies are limited by the number of identified linear epitopes in the past. Due to the limited number of combined epitopes, it is still difficult to achieve sufficient preventive or therapeutic effects of synthetic peptide vaccines at the level of linear antibodies.

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