50 results about "Bovine oocyte" patented technology

The present invention provides an in vitro mature culture fluid containing tea-polyphenol for bovine ovocyte and its culture method. The described in vitro mature culture flud uses conventional culture fluid as matrix, in the described matrix 20-150 microgram / mL of tea-polyphenol, 100 IU / mL of penicillin, 100 microgram / mL of streptomycin and serum whose volume percentape content is 8%-20% are also contained. Said invention can make tea-polyphenol be used in the field of bovine ovocyte in vitro mature culture so as to raise early embryonic in vitro development rate after the bovine ovocyte in vitro fertilization.

The invention relates to a method of producing a protein of interest, comprising making a non-human transgenic mammal that produces said protein in its milk, obtaining said milk from the non-human transgenic mammal and purifying said protein of interest from the milk. Transgenic bovine animals were generated, which are able to produce human growth hormone in mammary glands. The method involves cloning of a genetic construct encoding hGH gene and beta casein promoter conveniently in an expression vector. It also includes transfection procedures into fetal bovine somatic cells, generally fibroblasts, and the nuclear transfer into enucleated bovine oocytes, generating thus transgenic embryos. The method also includes other procedures to generate transgenic embryos for the further expansion of the transgenic herd, such as the subcloning of transgenic female bovines, the superovulation of transgenic cows and their insemination with semen from a non-transgenic or a transgenic male bovine, and the superovulation of non-transgenic cows and their insemination with semen from a transgenic male bovine. Afterwards, transgenic embryos give rise to transgenic cattle that produce human growth hormone in huge amounts in their milk, from which the hormone is completely purified and analysed to fulfill all the requirements for the manufacture of a pure biopharmaceutical product.

The invention provides novel methods for IVM of bovine oocytes. These methods can be used in efficient propagation of superior animals at low cost, which is very helpful in improving ecological and economic performance in the cattle industry.

The invention provides bovine oocyte in-vitro mature culture solution containing mycose and a cultural method. The bovine oocyte in-vitro mature culture solution comprises 0.1-1.0mg / mL of mycose, 0.1-10mu g / mL of daidzein, 100IU / mL of penicillin, 100mu g / mL of streptomycin and 8% to 20% serum, wherein the solvent is regular nutrient solution which is one or more of M199, DMEM, F12 and MEM. The combination of mycose and daidzein is applied to bovine oocyte in-vitro mature culture, the cytoplasm maturing of oocyte can be remarkably promoted in comparison with solely using mycose or daidzein, and the problem of low embryo in-vitro developmental rate after the in-vitro mature oocyte is fertilized. The bovine oocyte in-vitro mature culture solution is low in economical cost and convenient to popularize and applied to production.