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Bovine oocyte in vitro maturation culture solution and culture method

A technology for in vitro maturation and culture of oocytes, applied in the field of in vitro maturation culture medium for bovine oocytes, can solve the problems of low oocyte development ability, improve the quality of maturation, improve the rate and quality of early embryo development, and development ability enhanced effect

Active Publication Date: 2018-06-01
NORTHWEST A & F UNIV
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Problems solved by technology

In vitro maturation of oocytes is not only affected by various factors such as ovarian cycle, animal species, follicle size, hormones, serum, etc., but also closely related to the procedures and methods of in vitro maturation of oocytes and the composition of the culture medium. Great progress has been made, but the developmental ability of in vitro matured oocytes is significantly lower than that of in vivo matured oocytes

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  • Bovine oocyte in vitro maturation culture solution and culture method
  • Bovine oocyte in vitro maturation culture solution and culture method
  • Bovine oocyte in vitro maturation culture solution and culture method

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Embodiment Construction

[0018] The present invention will be described in detail below in conjunction with the embodiments.

[0019] The experiment found that CXCL12 exists in high abundance in bovine follicular fluid, so it is speculated that this factor (CXCL12) may promote the maturation of oocytes. Therefore, the present invention improves the in vitro maturation culture medium by adding components such as CXCL12 to improve the maturation quality of bovine oocytes cultured in vitro.

[0020] (1) Bovine oocyte maturation medium in vitro

[0021] Bovine oocyte maturation medium in vitro includes M199 basal medium without Hepes (4-hydroxyethylpiperazineethanesulfonic acid) and bFGF (basic fibroblast growth factor), EGF ( epidermal growth factor), 17β-estradiol, HMG (human menopausal gonadotropin), fetal bovine serum, ITS and CXCL12.

[0022] In one embodiment of the present invention, the bovine oocyte maturation medium in vitro contains 10% fetal bovine serum and 1% ITS-G (human source), and cont...

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Abstract

The invention provides a bovine oocyte in vitro maturation culture solution and a culture method. The maturation culture solution consists of 10% of fetal calf serum and 1% of ITS-G, as well as 0.075IU / mL of HMG, 1[mu]g / mL of 17[beta]-estradiol, 10ng / mL of EGF, 2.2mg / mL of bFGF and 50ng / mL of CXCL12. The culture solution, when applied to bovine oocyte in vitro maturation culture, can improve maturation quality of bovine oocyte in vitro culture, a development rate of in vitro fertilized embryos as well as a development rate of somatic cell nuclear transplantation embryos and blastocyst quality.

Description

technical field [0001] The invention relates to the technical field of livestock embryo engineering, in particular to a culture medium for in vitro maturation of bovine oocytes and a method for cultivating bovine oocytes using the culture medium. Background technique [0002] In vitro maturation and culture of oocytes is the basis and key link of embryo engineering technologies such as in vitro fertilization, somatic cell nuclear transfer, in vitro production of animal embryos, and transgenic cloning. The mature quality of oocytes directly determines the developmental potential of embryos after fertilization or nuclear transfer. . In vitro maturation of oocytes is not only affected by various factors such as ovarian cycle, animal species, follicle size, hormones, and serum, but also closely related to the procedures and methods of in vitro maturation of oocytes and the composition of the culture medium. Great progress has been made, but the developmental ability of oocytes ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/075
CPCC12N5/0609C12N2500/25C12N2501/11C12N2501/115C12N2501/21C12N2501/31C12N2501/392
Inventor 张景程张涌马晓楠张敏王勇胜
Owner NORTHWEST A & F UNIV
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