In-vitro culture system for Tibetan yak oocyte
A technology for in vitro culture of oocytes, applied in the field of Tibetan yak oocyte in vitro culture system
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
experiment example 1B
[0025] Experimental example 1 Effect of BMP15 on maturation of yak granulosa cells and their oocytes in vitro
[0026] 【Objective】The purpose of this study was to investigate the effects of bone morphogenetic protein 15 (BMP 15) on the proliferation of yak granulosa cells (Granulosa Cells, GCs) and the culture of oocytes (oocytes, COCs) in vitro. [Method] The ovaries of healthy yaks were taken, and COCs and GCs were isolated for in vitro maturation culture; 0, 10, 50, 100 ng·mL were added to the yak maturation medium -1 After BMP15, the proliferation of yak GCs was detected by the CCK-8 method and the oocyte maturation rate was counted; then the downstream molecule Smad 4 of the signaling pathway and the cumulus expansion marker genes HAS 2 and PTX 3 in COC treated with BMP15 were detected by Real-time PCR method , the expression level of PTGS2. 【Result】Added 0, 10, 50, 100 ng·mL to the culture of yak GCs -1 After BMP15, the results showed that adding 100ng·mL -1 The cell p...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com