Application of sulforaphane in preparation of bovine oocyte in-vitro maturation solution
A technology of sulforaphane and oocytes, applied in the biological field, can solve problems such as no related reports, achieve the effect of expanding the scope of application, improving the quality of in vitro maturation of oocytes, and increasing the rate of in vitro maturation
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[0025] 1. In vitro maturation of oocytes
[0026] (1) Collection of oocytes
[0027] The ovaries were collected from the slaughterhouse of Dachang County, Langfang City, Hebei Province, placed in sterilized normal saline containing double antibodies at 28-30°C, and transported back to the laboratory within 2 hours. Wash the ovaries 2 to 3 times with sterilized saline preheated at about 30°C in advance, and use a vacuum peristaltic pump to extract the follicle fluid in follicles 3 to 6 mm above the ovary. Cumulus-oocyte-complexes (COCs) wrapped by more than two layers of dense cumulus cells were used for in vitro maturation.
[0028] (2) In vitro maturation of oocytes
[0029] The selected COCs were washed twice in the egg washing solution, and then washed twice in the maturation solution, and transferred into the CO 2 In the four-well plate that has been equilibrated for at least 2 hours in the incubator (750 μL maturation solution per well, 50 COCs), the maturation solutio...
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