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Application of sulforaphane in preparation of bovine oocyte in-vitro maturation solution

A technology of sulforaphane and oocytes, applied in the biological field, can solve problems such as no related reports, achieve the effect of expanding the scope of application, improving the quality of in vitro maturation of oocytes, and increasing the rate of in vitro maturation

Active Publication Date: 2021-08-13
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows researchers to study how Sulfuric Acene works when used alone or together with other agents like MT to improve cellular metabolism during pregnancy. It does this through increasing the amount of certain compounds that are found naturally occurring within cells called Selenoxins. These chemicals help protect these specialized molecules against oxidative damage caused by reactive free radical species generated during laboratory conditions such as high temperatures. Overall, it enhances the ability of spermatozoa to produce viable offspring after birth without causing any harmful side-effects associated therewith.

Problems solved by technology

This patented technical issue addressed in the patented text relates to the lack of effective methods for producing stable human chickens from these crops without compromising upon resistance against herbivores like tigergrastrointestinalis and hypothyroidisms. Current techniques involve expensive processes requiring multiple steps before reaching optimal levels of nutrition and sterility.

Method used

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  • Application of sulforaphane in preparation of bovine oocyte in-vitro maturation solution
  • Application of sulforaphane in preparation of bovine oocyte in-vitro maturation solution
  • Application of sulforaphane in preparation of bovine oocyte in-vitro maturation solution

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Embodiment 1

[0025] 1. In vitro maturation of oocytes

[0026] (1) Collection of oocytes

[0027] The ovaries were collected from the slaughterhouse of Dachang County, Langfang City, Hebei Province, placed in sterilized normal saline containing double antibodies at 28-30°C, and transported back to the laboratory within 2 hours. Wash the ovaries 2 to 3 times with sterilized saline preheated at about 30°C in advance, and use a vacuum peristaltic pump to extract the follicle fluid in follicles 3 to 6 mm above the ovary. Cumulus-oocyte-complexes (COCs) wrapped by more than two layers of dense cumulus cells were used for in vitro maturation.

[0028] (2) In vitro maturation of oocytes

[0029] The selected COCs were washed twice in the egg washing solution, and then washed twice in the maturation solution, and transferred into the CO 2 In the four-well plate that has been equilibrated for at least 2 hours in the incubator (750 μL maturation solution per well, 50 COCs), the maturation solutio...

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Abstract

The invention provides an application of sulforaphane in preparation of a bovine oocyte in-vitro maturation solution. The invention provides the culture solution for promoting in-vitro maturation of the bovine oocytes, and the sulforaphane in the culture solution has an obvious promoting effect on in-vitro maturation and in-vitro developmental ability of the bovine oocytes.

Description

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Claims

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Application Information

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Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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