86 results about "Festuca elata" patented technology

The invention discloses a method for biologically remediating a water body and soil comprehensively utilizing resources. The method comprises the steps: (1), determining main pollutants in a selected land area or water body area; (2), selecting to plant and / or cultivate fast-growing herbaceous plants, fast-growing alga, trees, bushes, fungi or microorganisms with high remediation efficiency in the selected land area or water body area; (3), harvesting or collecting the fast-growing herbaceous plants and the fast-growing alga after growing to reach a suitable height or size; (4), concentratedly processing the harvested or collected fast-growing herbaceous plants and the fast-growing alga, and comprehensively utilizing to prevent the pollutants from dispersing; (5), remediating an eutrophic water body by adopting an artificial floating island; (6), remediating a heavy metal polluted water body by confining floating plants; (7), repairing cadmium and zinc polluted soil by mixed-cropping festuca arundinacea and bluegrass; (8), remediating polycyclic aromatic hydrocarbons (PAHs) polluted soil by mixed-cropping alfalfa and italian ryegrass; and (9), extracting and recovering silver from silver-containing wastewater remediation plants.

The invention relates to a method for improving activity of antioxidant enzyme of festuca arundinacea by using fine domestic garbage compost under drought conditions. In the method, a plastic pot with diameter of 7cm and height of 10cm is used through a pot experiment; and the method comprises the steps of: putting 150g of soil into each pot; sowing 0.5g of lawn plants in each pot; uniformly spreading 3g of municipal domestic garbage fine compost with particle sizes of 1,200nm, 600nm and 300nm on the surface of the soil; uniformly covering 20g of soil on the surface of the soil; controlling the temperature to be between 20 and 26 DEG C, wherein the relative humidity is between 40 and 60 percent, and illumination is natural light transmitting indoors; fully watering the plants in the initial stage of sowing, and watering by weighing every day to every two days according to 55 to 65 percent of water-holding capacity in fields after two weeks so as to maintain the capacity within a stress range; and measuring all the indexes on the 44th day. A result shows that the fine compost protects the festuca arundinacea plant from being damaged by active oxygen radicals, and reduces adverse environmental stresses to the plant caused by drought stresses.

The invention discloses a method for controlling the growth of compost substrate festuca arundinacea by using modified nano carbon. The method comprises the steps of: sealing the lower end of each PVC pipe of which the diameter is 3cm and the height is 25cm with a layer of cotton cloth and a nylon net, mixing garbage compost and all modified nano carbons uniformly in proportion, and charging 150g of mixed material into each pipe; passivating for 7 days, sowing 0.2g of festuca arundinacea seeds in each pipe, controlling the temperature to be 19-27 DEG C and the relative humidity to be 60-72% during experiment, supplementing water to the compost every day so that the moisture of the compost reaches 70% of field capacity, balancing for 30 days in a laboratory, and measuring; wherein the adding amount of each modified nano carbon is 1-5%(w / w) of the weight of the garbage compost. Experimental results show that the growth of the compost substrate festuca arundinacea is promoted by adding the modified nano carbon.

The invention discloses a regulation and control method for improving diversity of nanocarbon to soil nematodes. The method comprises the steps of adopting plastic flowerpots of which the lengths are 25cm and the diameters are 2cm, cleaning and drying gauzes, fixing each gauze which is cut to a round shape with a proper size at the bottom of each flowerpot, and weighing matrix garden soil of 2kg in each container, wherein modified nanocarbon of which the weight is 1-5% that of the matrix garden soil is added into the garden soil respectively; respectively sowing festuca arundinacea seeds of 4.5g respectively; regularly quantitatively watering in the experiment period every day to meet the plant growth demands, culturing the plants in a laboratory, wherein during the cultivation period, the temperature is 25-34 DEG C, the relative humidity is 31-48%, the lighting condition is natural incident light, the experiment time is 120 days, the nematodes in the soil are separated to identify the type and number of the nematodes as the background data of the nematodes, and the type and number of the nematodes are processed after separation and identification after 4 months.

The invention relates to a method for improving growth performance of lawn plants by adopting rare earth lanthanum. The method comprises the following steps of: soaking seeds of lawn plants with lanthanum nitrate water solution at a concentration of 50mg.L<-1>-300mg.L<-1> for 18-24 hours; then sowing the seeds in a culture dish with filter paper spread, and putting the culture dish with the sowed seeds in the sun to germinate the seeds; beginning recording the germination number 2-3 days after sowing the seeds; and after grass seed growth ends, cutting off the plants at ground level with scissors, measuring the fresh weight of the single plant and the total fresh weight, drying the samples in a drying box at 80 DEG C for 8 hours, and measuring each index. Adopted to soak the seeds, the rare earth lanthanum has an influence on germination and initial growth of Lolium perenne L. and Festuca arundinacea.L, has an effect on promoting germination of the two grass seeds, and has an obvious effect on promoting growth of the underground root system parts. Therefore, the research results show that the rooting degrees and green degrees of the grass seeds on the lawns are regulated to achieve the best effects by soaking the seeds with the rare earth element.

The invention discloses a method for concentrating soil heavy metals of a sewage irrigation area by coalition of a composite restoring agent and festuca arundinacea, wherein the composite restoring agent consists of a chelating agent nitrilotriacetic acid (NTA) and a surfactant sodium dodecyl sulfate, wherein the mol ratio of the chelating agent nitrilotriacetic acid to the surfactant sodium dodecyl sulfate is (5-15) mmol.kg<-1>:1mmol.kg<-1>. Experimental results show that a most remarkable effect of increasing the content of Cd in the festuca arundinacea is achieved due to the addition of the surfactant and the chelating agent, the concentration of the heavy metals can be promoted through co-addition of the surfactant and the chelating agent, and especially the effect is more remarkable when 5 mmol.kg<-1> of the NTA and 1 mmol.kg<-1> of the chelating agent or 10 mmol.kg<-1> of the NTA and 1 mmol.kg<-1> of the chelating agent are used for restoring sewage irrigation soil.

The invention discloses a low-temperature resisting method for festuca arundinacea by using air-dried sludge inoculants, which is implemented by comprising components including pseudomonas stutzeri, trichoderma reesei and yellow streptomyces 100-times diluent or pseudomonas stutzeri, trichoderma reesei and yellow streptomyces 200-times diluent. Development results of the invention show that the application of the complex microbial inoculants of pseudomonas stutzeri, trichoderma reesei and streptomyces griseoflavus can significantly improve the height, the aboveground biomass and the chlorophyll content of festuca arundinacea plants, and improve the cold resistance thereof. The regreening time of festuca arundinacea to which the complex microbial inoculants are applied is about five days earlier than that in a blank, and the comprehensive index of lawn appearance of regreened festuca arundinacea is also significantly higher than that in the blank, which fully shows the application potential of the complex microbial inoculants in practice.

The invention discloses a method for utilizing cadmium-resisting fungus aspergillus aculeatus for promoting festuca arundinacea to remedy cadmium contaminated soil, and relates to the technical field of plant-microorganism combined ecological remediation. The method mainly comprises the steps that firstly, cadmium-resisting fungus aspergillus aculeatus separated, purified and identified out of rhizosphere soil of dominant species in heavy metal contaminated soil is bred and inoculated into crushed crop straw to be prepared into a microbial remediation matrix, and the microbial remediation matrix is applied into cadmium contaminated soil; secondly, a festuca arundinacea lawn is planted in a sowing manner; thirdly, watering of a citric acid aqueous solution of a proper concentration and a proper quantity is carried out in batches; and fourthly, the rhizosphere microenvironment of festuca arundinacea is adjusted and controlled through citric acid to promote the interaction of cadmium-resisting fungus aspergillus aculeatus and a root system of festuca arundinacea so as to improve cadmium remediation efficiency. The problem of adaptability of screened-out microorganisms to the soil environment is solved; transferring of cadmium towards ground parts of plants is promoted, and the cadmium remediation efficiency is improved; and good environmental benefits, economic benefits and social benefits are achieved.

The invention discloses a method for improving drought resistance of turf on garbage compost substrate, which includes: selecting a lawn plant Festuca arundinacea and domestic garbage compost, applying 0.2-0.4Xkg-1 of rare-earth lanthanum nitrate or cerium nitrate per 60g of the compost, and mixing for use; respectively scattering 0.5g of Festuca arundinacea seeds into cups; controlling largest water-holding capacity of the compost in a field to be 66% and severe stress to be 35-50%, normally supplying water early in the experiment to guarantee smooth seed germination and seedling growth, quantitatively feeding water after 2 weeks according to the degree of severe stress, and controlling the degree of drought by weighing process; and finally measuring all indexes. Experimental results show that applying rear earth increases the ratio of chlorophyll a, chlorophyll b, content of total chlorophyll and chlorophyll a / b of Festuca arundinacea under drought stress, so that optical absorption of Festuca arundinacea is enhanced, accumulation of photosynthate is promoted, and stress resistance of the lawn plant Festuca arundinacea is improved.

The invention discloses a method for improving chromium resistance and cadmium resistance of Festuca arundinace with compost fermentation liquid, which comprises the following steps: adding 200g of oven-dried soil into disposable plastic cups of a diameter 7cm and seeding 0.8g of Festuca arundinace seeds, respectively adding microbial inoculums to lawn plants after seeding for 10 days, respectively adding cadmium chloride solution and potassium dichromate solution to soil, repeating the experiment for three times (60 samples in all), continuing culturing at room temperature while feeding water at a fixed amount every day during the experiment to ensure uniform environment among treatment chambers, and measuring indicators after growing for 30 days. The experimental results shows that after the Bacillus thuringiensis preparation is added, the underground dry weight and the root-top ratio of Festuca arundinace are increased by 137.5% and 102.9% respectively compared with the underground dry weight and the root-top ratio of the control, and the lawn plant growth is promoted, and the chromium resistance and cadmium resistance of Festuca arundinace are remarkably improved.

The invention discloses a method of intensifying festuca arundinacea to repair heavy metals in soil in a sewage irrigation area by an NTA (Nitrilotriacetic Acid) and microbial agent. The agent consists of a chelating agent NTA and a microbial liquid, wherein the molar ratio of the chelating agent to the microbial liquid is 5-15mmol.kg<-1>NTA:15mmol.kg<-1>microbial liquid. The microbial liquid comprises pseudomonas stutzeri, trichoderma reesei and streptomyces griseoflavus which are mixed in a volume ratio of 1:1:1 to prepare a compound microbial liquid. The clump counts of pseudomonas stutzeri and streptomyces griseoflavus are respectively (2.15+ / -0.03)*10<9> / ml and (2.07+ / -0.07)*10<9> / ml, and the clump count of trichoderma reesei per ml spreads to the whole panel. The experimental results show that the effect of intensifying festuca arundinacea to repair heavy metals in soil in the sewage irrigation area is the best by combining 10mmol.kg<-1>NTA and 15mL of microbial liquid.

The invention discloses a method for regulating and controlling a festuca arundinacea lawn soil nematode community structure by adopting a complexant. The method comprises the following steps: adopting a plastic flowerpot with a height of 25 cm and a diameter of 12 cm, shearing a gauze into a circle with appropriate size to fix the bottom part of the flowerpot after cleaning and drying, sowing 4.5 g festuca arundinacea seeds in each container which weighs 2 kg, supplying water quantitatively everyday during the experiment to satisfy the need of the plant growth, adding EDTA ( Ethylene Diamine Tetraacetic Acid ),NTA ( Nitrilo Triacetic Acid ) or DTPA ( Diethylene Triamine Pentaacetic Acid ) with a concentration of 5 to 10 mmol / kg<-1>respectively, and culturing under the situations that the temperature is 25-34 DEG C, the relative humidity is 31-48%, the light condition is natural incident light, and the whole test lasts 120 days. The experiment result shows that the adoption of complexant increases the quantity of the soil nematodes on the whole, and particularly increases the quantity of the plant parasitic type nematodes; meanwhile the quantity of the bacteria eating type and polyphagia feeding type nematodes is reduced, thereby influencing the health of the soil ecosystem.

The invention discloses bipolaris panici-miliacei (Yi.Nisik.) Shoemaker (SX-5-2) and a screening method and application thereof. The bipolaris panici-miliacei (Yi.Nisik.) Shoemaker (SX-5-2) has been preserved in the general microbiological center of Chinese microbial species preservation management committee, and the preservation number is CGMCC No.13887. The concentration of conidium which utilizes the bipolaris panici-miliacei (Yi.Nisik.) Shoemaker (SX-5-2) is more than 104 per milliliter, and the bipolaris panici-miliacei (Yi.Nisik.) Shoemaker (SX-5-2) is used for biological weeding, can be safely used in Oryza sativa L., Triticum aestivum L., sorghum bicolor (L.) Moench, Glycine max (Linn.) Merr., Gossypium spp and vegetable crop fields as well as Festuca elata Keng ex E. Alexeev and Zoysia japonica Sreud., and is used for controlling gramineous weeds such as Digitaria sanguinalis (L.) Scop, Eleusine indica (L.) Gaertn, Setaria viridis (L.) Beauv., Echinoochloa crusgalli (l.) Beauv., Leptochloa chinensis (L.) Nees, Microstegium vimineum (Trin.) A. Camus, Alopecurus aequalis Sobol. and Poa annua L., as well as microstegium related genera plant such as Eccoilopus cotulifer (Thunb.) A., Miscanthus sinensis anderss., Eulalia speciosa, Microstegium japonicum, Arthraxon hispidus (Thunb.) makino, Sorghym sudanense (Piper stapf). The controlling effect on target weeds can reach more than 80%. The bipolaris panici-miliacei (Yi.Nisik.) Shoemaker (SX-5-2) has the characteristics of safety and environmental protection.

A glyphosate-tolerant tall fescue (Festuca arundinacea) variety in one form known as Tomahawk RT (experimental code PST-5MU), seed used to produce the grass, and methods of using the grass plant and the seed are provided. This grass is suitable for use in lawns, golf courses, sod, and other turfs where weeds are a problem. Weed control in areas planted with the disclosed grasses can be achieved bydirect application of glyphosate herbicides.

The invention discloses a method for restoring heavy metal in sewage irrigation soil through NTA and 3-indoleacelic acid (IAA)-enhanced festuca arundinacea. A mixture comprises chelating agent nitrilotriacetic acid and 3-indoleacelic acid in a mole ratio of 6-12mmol.kg<-1> to 2mg.kg<-1>. Experimental results show that under certain concentration, the plant toxicity of heavy metal and the chelating agent on festuca arundinacea can be relieved through the addition of IAA, and the accumulation of the plant on the heavy metal can be facilitated; the improvement of the above-ground biomass of the festuca arundinacea can be promoted through combination of the NTA and 3-indoleacelic acid. By comparing the combination of the IAA and NTA with the control group, the content of above-ground heavy metal of the festuca arundinacea can be increased, and therefore, more heavy metal can be extracted and accumulated by lawn plant, and the heavy metal-polluted soil can be restored to the maximum extent.

The invention relates to a method for improving chlorophyll fluorescence power of festuca arundinacea under a dry condition by cerium. According to the method, seeds of festuca arundinacea are sown on the garden soil subjected to lawn establishment, wherein every 100-200g of garden soil contains 200mg / Kg-800mg / Kg of cerous nitrate, then the chlorophyll fluorescence power parameters are measured under the normal water supply condition three weeks later; then moisture is controlled to the extent that the moisture content reaches the degree of moderate drought stress, and then the chlorophyll fluorescence power parameters are measured again seven days later; the moisture is controlled again to the extent that the moisture content reaches the degree of serious drought stress; and under the condition of serious drought stress, the chlorophyll fluorescence power parameters are measured for the last time seven days later. The plant chlorophyll fluorescence dynamics is a novel, quick, simple and accurate new technology, by which the physical state of photosynthesis of the plants can be detected without damaging the plants on the whole.

The invention discloses a method for improving cultivating property of Festuca arundinacea turf on garbage compost substrate, which includes: firstly, applying 0,2-0.6gXkg-1 of rear earth (lanthanum nitrate or cerium nitrate) per 20g of garbage compost; and secondly, sowing 50 Festuca arundinacea seeds into culture dishes, harvesting 31 days after germination, and measuring all indexes. Experimental results show that during replacing of soil with garbage compost for establishment of soilless turf, adding the rear earth helps lawn plant seeds germinate and grow in the early stage and increasesstress resistance of lawn. On the aspect of seed germination, low concentration lanthanum nitrate is equivalent with high concentration cerium nitrate in effect, and the effect is best when 0.2gXkg-1of lanthanum nitrate is used. Germination percentage, germination index and vitality index of Festuca arundinacea are respectively increased by 27.9%, 32.7% and 34.5% compared with those in control. The method provides theoretical basis for further application of rear earth to improving stress resistance of lawn plants on compost substrate.

The invention discloses a method for renovating compost leacheate with Festuca arundinacea and NTA (nitrilotriacetic acid), which includes adding NTA with 10mmol / kg-1 compost into 1500g of refuse compost, using 3000ml of tap water to soak for 72 hours, and making 25-100% compost leacheate; dipping seeds of Festuca arundinacea in a petri dish, measuring plant height after the seeds germinate, and clipping the plants for measuring dry weight of the plants 30 days of water culture; and using an atom absorption spectrophotometer TAS-990 to measure content change of heavy metals, namely Cu, Ni, Zn and Cr. Test results show that refuse compost in domestic refuse compost leacheate for cultivation of soilless turf is provided with additional chelant and contains organic matters, nitrogen, phosphorus and other nutrient elements, and accordingly the requirement of lawn plants on growth can be met sufficiently and reuse and safe discharge of the refuse compost leacheate are achieved.

The invention discloses a method for adjusting the water stress chlorophyll and the electric conductivity of festuca arundinacea by adopting a lawn nursery strip. The method comprises the following steps of: adding dried natural soil into a container by a potting method by taking waste newspaper and waste cotton cloth as a lower layer of a lawn nursery strip building carrier and taking waste gauze and waste face tissue as an upper layer of the lawn nursery strip building carrier; then paving the lawn nursery strip on the natural soil, and after seeds are planted, keeping a certain amount of water required by seed germination until the seeds germinate; after the seeds germinate for 5 days, starting to stress, and controlling the water content of the soil to be 35 to 50 percent of the saturated water-holding capacity; and after the seeds germinate for 40 days, measuring all physiological and biochemical indexes of festuca arundinacea seedlings, cutting the overground parts of the festuca arundinacea seedlings, keeping culturing for 40 days and harvesting eddish and the underground parts of the festuca arundinacea seedlings, wherein the total culture period lasts 80 days, the averagetemperature is 18 to 22 DEG C, and the average humidity is 30 to 40 percent. A test result shows that the waste lawn nursery strip exerts a great adjustment function in growth of grasses in a lawn when the plants are subjected to water stress.

The invention discloses a method for regulating and controlling the chemical properties of a land substrate for producing grass rolls. The method mainly comprises the following steps: in soil for planting previous crops festuca arundinacea, applying sawdust, turfgrass clippings, corn straws in the same year, composted corn straws and sludge nutritional soil according to a certain ratio into a turf production land, sowing festuca arundinacea barlexas II on mixed soil under the condition that the sowing quantity is 35 g / m<2>, and detecting the regulation and control effects of different supplemented waste supplementing materials on the pH value, the electric conductivity and the salt content of the soil after treating the festuca arundinacea. The technical ecological method for improving the chemical properties of the land substrate for producing the grass rolls opens up a new approach for resource utilization of the sawdust, the turfgrass clippings, the corn straws in the same year, the composted corn straws and the sludge nutritional soil.

The invention relates to a method adopting an ammonia carboxy chelating agent and festuca arundinacea to restore compost matrix heavy metals, which comprises adding 20g of prepared compost in each culture dish with the diameter to be 6cm, and adding the ammonia carboxy chelating agent with the concentration of 5-20mmol / kg<-1> into compost matrix; then selecting full festuca arundinacea seeds, enabling the number of the seeds in each dish to be 50; placing the culture dishes where the seeds are sown in a laboratory to germinate the seeds, watering the seeds for one time respectively in the morning and in the evening each day, often exchanging positions of the culture dishes to guarantee conformity of illumination, enabling the illumination strength during the culture period to be 350-800 mu mol / m<-2>s<-1>, enabling the temperature in the laboratory to be 16 DEG C to 24 DEG C, and enabling the relative humidity to be 38-55%; and adopting a TAS-990 atomic absorption spectrophotometer to determine content of various heavy metals. According to experimental results, on the upper portion of the festuca arundinacea, Cr is concentrated maximally when the concentration of the ammonia carboxy chelating agent is 10 mmol / kg<-1>, Cd, Cu and Zn are concentrated maximally when the concentration of the ammonia carboxy chelating agent is 15 mmol / kg<-1>, and Pb is concentrated maximally when the concentration of the ammonia carboxy chelating agent is 20 mmol / kg<-1>. Accordingly, the concentration of nitrilotriacetic acid (NTA) should be changed in selection according to differences of different plants in concentrating the heavy metals.

The invention discloses a method for regulating growth of festuca arundinacea in a hot summer environment by a sludge mixed seed soaking solution, which comprises the following steps: the sludge / rare-earth mixed seed soaking solution is composed of a sludge leaching solution and a rare-earth solution in a weight ratio of 1:1, wherein the sludge leaching solution is prepared by mixing sludge and distilled water in a weight ratio of 1:2, standing for 24 hours and filtering; and the rare-earth solution is a water solution containing 200-400 mg.L<-1> Ce(NO3)3.6H2O or La(NO3)3.6H2O. The experimental result indicates that the sludge mixed seed soaking solution can induce the accumulation of the seedling in-vivo proline, and can maintain the proline on a high level in the strong light and heat activation process, thereby enhancing the growth property of the festuca arundinacea in the field under high-temperature conditions in summer.

The invention discloses a composite chemical agent, which is prepared from a chelating agent nitrilotriacetic acid and a surfactant sodium dodecyl sulfate, wherein the molar ratio of the chelating agent nitrilotriacetic acid to the surfactant sodium dodecyl sulfate is 5-15 mmol.kg<-1> nitrilotriacetic acid (NTA) to 1 mmol.kg<-1> sodium dodecyl sulfate. On the basis of an optimal experiment, the growth of festuca arundinacea in sewage irrigation area soil is regulated and controlled by combination of the NTA and the surfactant. An experiment proves that the method for improving festuca arundinacea growth in the sewage irrigation area soil by using the composite chemical agent can be applied to reduction of the toxicity of the festuca arundinacea caused by heavy metal. The method has positive meaning on production of carpet grass in the sewage irrigation area soil.

The invention discloses an improved method for compacted soil in a surface mine dump. According to the method, perennial gramineous plants including BromusinermisLeyss., Agropyroncristatum(L.)Gaertn,FestucaelataKeng ex E.Alexeev and Leymuschinensis(Trin.)Tzvel. are mixed with leguminous plants including Medicago falcata L. and Melissilusruthenicus(L.)Peschkova(TrigonellaruthenicaL.) to serve as acolonization plant which cooperates with arbuscular mycorrhizal fungi. The method provided by the invention can provide effective help for vegetation ecological restoration of the surface mine dump and can significantly reduce the compaction degree of the compacted soil, so that the method can be popularized.

The present invention provides a feed for stock rams in a mating period. The feed comprises corns, linseed cakes, rapeseed cakes, black beans, carrots, chrysanthemum coronarium, kalimeris indica, pennisetum purpureum, alfalfa, Gandancao (a hybrid of sorghum and sorghum sudanense), pennisetum typhoideum, ryegrass, festuca elata, fish sauce, fish bone meal, shell powder, salt, traditional Chinese medicinal additives, nutritional additives and a drying agent. The feed for the stock rams in the mating period is suitable to be added in the short mating period of the stock rams, reasonable in matching, and rich in nutrition and various proteins, vitamins, minerals, etc. The added traditional Chinese medicinal additives and nutritional additives can enhance physical fitness, improve sexual desires and breeding ability, improve the activity and quality of goat sperms, and meet the high demands in the mating period.

The invention discloses a method for using Bacillus thruingiensis fermentation liquor to adjust protective enzymes in Festuca arundinacea, which includes: adding 30g of lawn soil into each petri dish 6.5cm in diameter; respectively adding 150 microliters of compost microbial fermentation filtrates which are in different concentrations and contain Bacillus thruingiensis into the soil; adding aseptic liquid medium into a control group; evenly spreading 50 full seeds of Festuca arundinacea into each petri dish for planting at room temperature; and testing germination indicators after the seeds germinate. Test results show that application of the compost microbial fermentation filtrates lowers activity of peroxidase, superoxide dismutase and catalase in lawn plant leaves, the content of malondialdehyde and the content of free proline accumulated in lawn plants, and compost microbes have certain relief effect for plants growing in adverse environment.

The invention discloses an extraction method for endophytic fungi in festuca elata Keng ex E.Alexeev leaves. The method includes taking a festuca elata Keng ex E.Alexeev leaf, and placing the leaf ina sterile console for 15-20 hours; putting the leaf in an alcohol solution with 75% to clean for 30-45 sec, then putting the leaf in a sodium hypochlorite solution with 0.5% to clean for 10-15 sec, and putting the leaf on a sterile paper to dry for later use after cleaning the leaf for 2-3 times with clear water; performing hole punching on the leaf by using a hole punch, and placing the obtainedcircular leaf on a culture medium of a glass dish; sealing the glass dish, and performing fusion on a sealing strip through an alcohol burner; placing the glass dish in a darkroom under an environmentof 25 DEG C to culture for 10-15 days so that endophytic fungal colonies can be obtained; and picking out the colonies by using an inoculating needle to perform individual culturing so that endophytic fungus strains can be obtained. Compared with the prior art, the method has characteristics of high fungi extraction efficiency, high extraction survival rate and low cost.

The invention discloses a method for regulating proline and malondialdehyde of Festuca elata Keng subjected to water stress by adopting sodded strips. The method comprises the steps of: using waste newspaper and cotton cloth as the lower layer of a carrier on which sodded strips are planted, and using waste gauze and tissue paper as the upper layer of the carrier on which the sodded strips are planted; by adopting the pot-culture method, adding dried raw soil in a container, then flatly laying the sodded strips on the raw soil, guaranteeing the amount of water required by seed germination after sowing until germination; beginning to stress after 5d of germination, and controlling the soil water content at 35 percent to 50 percent of the saturated water-holding capacity; and after 40 days of germination, measuring various physiological and biochemical indicators of young seedlings of Festuca elata Keng, cutting the part of the Festuca elata Keng on the ground, continuing to culture for 40 days, and harvesting the second-crop grass and the underground part, wherein the total culture period is 80 days, the average temperature is 18-22 degrees, and the average humidity is 30-40 percent. As proven by the experimental result: when plants are subjected to water stress, the sodded strips planted on wastes have good regulating effect on the growth of lawn grass.

The invention discloses a method for improving sod production drought resistance by adopting an enhanced garbage compost microbial agent. The enhanced drought-resistant microbial agent comprises enhanced bacillus cereus, enhanced lysine bacillus and enhanced rhodotorula gracitis in the weight ratio being 1:1:1. Bacillus cereus, lysine bacillus and rhodotorula gracitis are enhanced by gradually increasing concentration of PEG (polyethylene glycol) 6000, in the enhancing process, the concentration of PEG 6000 is gradually increased with increase of OD (optical density) 600 on the gradient of 50g / L and grows towards the ultimate goal of 250g / L; the concentrations of PEG 6000 are 5% (w / w), 10% (w / w), 15% (w / w), 20% (w / w) and 25% (w / w) respectively; after each time of increase of the concentration of PEG 6000 and stable growth increment of thalluses, the concentration of the PEG 6000 is continuously increased, and the drought-resistant mixed microbial flora are enhanced stage by stage. Meanwhile, the invention discloses an application of the enhanced drought-resistant microbial agent in improving biomass of festuca arundinace under the drought stress.