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Method of intensifying festuca arundinacea to repair heavy metals in soil in sewage irrigation area by NTA (Nitrilotriacetic Acid) and microbial agent

A technology of microbial bacterial liquid and composite microbial bacteria, which is applied in the field of remediation of heavy metals in soil in sewage irrigation areas

Inactive Publication Date: 2014-07-16
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no literature report on the study of combining nitrilotriacetic acid (NTA) with microorganisms and plants extracted from municipal sludge to remediate heavy metal pollution in sewage irrigation soil

Method used

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  • Method of intensifying festuca arundinacea to repair heavy metals in soil in sewage irrigation area by NTA (Nitrilotriacetic Acid) and microbial agent
  • Method of intensifying festuca arundinacea to repair heavy metals in soil in sewage irrigation area by NTA (Nitrilotriacetic Acid) and microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] 1 Materials and methods

[0081] 1.1 Test material

[0082] Municipal sludge comes from Tianjin Jizhuangzi Sewage Treatment Plant.

[0083] 1.2 Culture of strains

[0084] Weigh 10 g of sludge, put it into a conical flask filled with 90 g of sterile water, add glass beads, and shake on a shaker for 20 min. Prepared by dilution separation method 10 －2 , 10 －3 、10 －4 、10 －5 、10 －6 sludge suspension. According to the isolated microorganisms, take different concentrations of sludge suspension (fungi take 10 －2 、10 －3 、10 －4 , Actinomycetes take 10 －3 、10 －4 、10 －5 , bacteria take 10 －4 、10 －5 、10 －6 ), shake well and pipette 0.2 mL into the pre-poured plate. The fungus was plated on Martin's agar, and 1000 ml of this culture solution was added with 3.3 ml of 1% Bengal red aqueous solution. Add 0.3 ml of 1% streptomycin solution to every 100 ml medium before use; use Gaoshi No. 1 agar plate for actinomycetes, add 2 drops of 10% phenol to every 100 ml medi...

Embodiment 2

[0136] The method of strengthening tall fescue with bacterial agent containing NTA and microbial liquid to restore heavy metals in soil of sewage irrigation area:

[0137] (1) Handling of materials:

[0138] Lawn Plant Tall Fescue ( Festuca arundinacea L . ) is the experimental material;

[0139] The bacterial liquid is Pseudomonas stutzeri, Trichoderma reesei and Streptomyces grisea, mixed according to the volume ratio of Pseudomonas stutzeri, Trichoderma reesei and Streptomyces grisea and flavum, and prepared into a compound Microbial bacteria liquid; prepared into a composite microbial bacterial liquid, wherein the colony numbers of Pseudomonas stutzeri and Streptomyces grisea and flavum were respectively (2.15±0.03)×10 9 / ml, (2.07±0.07)×10 9 / ml, the number of colonies per ml of Trichoderma reesei spread across the entire plate.

[0140] Chelating agent selects nitrilotriacetic acid for use;

[0141] After the grass roots and stones were removed, the collected soil...

Embodiment 3

[0146] The method of strengthening tall fescue with bacterial agent containing NTA and microbial liquid to restore heavy metals in soil of sewage irrigation area:

[0147] (1) Handling of materials:

[0148] Lawn Plant Tall Fescue ( Festuca arundinacea L . ) is the experimental material;

[0149] The bacterial liquid is Pseudomonas stutzeri, Trichoderma reesei and Streptomyces grisea, mixed according to the volume ratio of Pseudomonas stutzeri, Trichoderma reesei and Streptomyces grisea and flavum, and prepared into a compound Microbial bacteria liquid; prepared into a composite microbial bacterial liquid, wherein the colony numbers of Pseudomonas stutzeri and Streptomyces grisea and flavum were respectively (2.15±0.03)×10 9 / ml, (2.07±0.07)×10 9 / ml, the number of colonies per ml of Trichoderma reesei spread across the entire plate.

[0150] Chelating agent selects nitrilotriacetic acid for use;

[0151] After the grass roots and stones were removed, the collected soil...

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Abstract

The invention discloses a method of intensifying festuca arundinacea to repair heavy metals in soil in a sewage irrigation area by an NTA (Nitrilotriacetic Acid) and microbial agent. The agent consists of a chelating agent NTA and a microbial liquid, wherein the molar ratio of the chelating agent to the microbial liquid is 5-15mmol.kg<-1>NTA:15mmol.kg<-1>microbial liquid. The microbial liquid comprises pseudomonas stutzeri, trichoderma reesei and streptomyces griseoflavus which are mixed in a volume ratio of 1:1:1 to prepare a compound microbial liquid. The clump counts of pseudomonas stutzeri and streptomyces griseoflavus are respectively (2.15+ / -0.03)*10<9> / ml and (2.07+ / -0.07)*10<9> / ml, and the clump count of trichoderma reesei per ml spreads to the whole panel. The experimental results show that the effect of intensifying festuca arundinacea to repair heavy metals in soil in the sewage irrigation area is the best by combining 10mmol.kg<-1>NTA and 15mL of microbial liquid.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and relates to a method for strengthening tall fescue with NTA and microbial agents to restore heavy metals in soil in sewage irrigation areas. Background technique [0002] Sewage contains a large amount of heavy metal elements, and accumulation in the soil will affect the absorption and utilization of nutrients by plants, as well as disturb the metabolic balance of plants. Field experiments in the suburbs of Beijing showed that sewage irrigation affected the plant height, leaf Area index, resulting in a significant reduction in yield and dry matter. The pollution of crop quality in sewage irrigation areas is mainly manifested in the excessive heavy metal content and the change of nutritional components in agricultural products. Studies have shown that the various quality indicators of rice in mud fields irrigated with sewage are significantly lower than those in mud fields irr...

Claims

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Application Information

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IPC IPC(8): C09K17/00B09C1/08B09C1/10
Inventor 赵树兰多立安常晨
Owner TIANJIN NORMAL UNIVERSITY
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