Provided is a herbicidal composition containing saflufenacil, glyphosate and fluazifop-p-butyl. A mass ratio of effective components including the saflufenacil, the glyphosate and the fluazifop-p-butyl in the herbicidal composition is 1-50:0.1-20:0.1-20; a total mass of the effective components in the composition accounts for 1%-60% of a total mass of the composition, and the rest components are permitted and acceptable auxilairy components in pesticide; and the formulation form of the herbicidal composition can be emulsifiable concentrate, suspension concentrate, wettable powder, water dispersible granules, aqueous emulsion, microemulsion, granules, microcapsules or water aqua. Main weeds in rice fields, such as barnyard grass, leptochloa chinensis, digitaria sanguinalis and goosegrass herbs, and winter wheat weeds such as amur foxtail, avena fatua, polypogon fugax, bluegrass, sclerochloa dura, puccinellia tenuiflora, Italian ryegrass, lolium temulentum, bromegrass, phleum paniculatum huds, aegilops tauschii, beckmannia syzigachne, wheatgrass, shepherd's purse, descurainia sophia and crickweed, can be prevented and removed effectively.