118 results about "Lolium multiflorum" patented technology

A method for raising high-quality meat goats relates to a raising method for goats, in particular to a raising method for increasing the meat of high-quality meat goats. The purpose of the present invention is to provide a goat raising method with high meat production rate and high slaughter rate, which combines grazing and supplementary feeding. The feeding method of the present invention combines supplementary feeding with grazing, prolonging grazing time in summer and autumn without supplementing or less supplementing feed, supplementing and supplementing more feed in spring and winter, and the feed includes green feed and mixed feed, green feed It refers to all kinds of forages on grassy hillsides, consisting of crop straws, miscellaneous grains, artificially planted forages such as ryegrass, clover, alfalfa, A. alfalfa, Mexican corn, etc. commonly used in rural areas; The needs of the feeding stage of the goat are matched.

The invention provides a shrub-grass mixed slope ecological repairing method, which is characterized in that after the slope surface is cleaned up, a suspended net soil spray-sowing method or a normal spray-sowing method is adopted so as to spray-sow the coverage material mixed by spray-sowing layer material containing shrub plants and herb seeds onto the slope surface and carry out the cultivation management finally; wherein, the mass ratio of the shrub plants and the herb seeds is 1-10:1; the sowing quantity of the seed is 15-35g / m<2>; the shrub plant is chosen from one or more plants outs of oleander, smoke tree, lespedeza floribunda, pyracantha, pittosporum or indigofera pseudotinctoria; the herb plant is chosen from one or more plants out of mother chrysanthemum, white clover, alfalfa, bermuda grass, red fescue or italian ryegrass. The slope ecological repairing afforestation mode of the invention leads the shrub plant to be combined with the herb plant, optimizes the ecological benefits and landscape effects, can achieves the object of realizing the soil and water conservation for the slope, and can beautify the slope environment and create graceful rural scenery.

The invention discloses a method for removing organic particles and nutritive salt in closed cycling aquaculture water. The method is characterized by comprising the following steps: establishing a plant filter chamber communicated with a culture pond in a closed cycling aquaculture system; planting 3.0 to 4.5m2 of myriophyllum aquaticum or Italian ryegrass in the plant filter chamber on the basis of per cubic meter of the aquaculture water; placing the plant filter chamber in a sunlight room with humidity of between 70 and 80 percent; controlling the illumination intensity of the plant filter chamber to be between 8,000 and 25,000lux and the aquaculture temperature to be between 15 and 35 DEG C; driving the aquaculture water to cycle to ensure that the aquaculture water flows through the plant filter chamber and flows back to the culture pond and the daily circulation quantity of the aquaculture water is 6 to 10 times of the volume of the aquaculture water. The method has the advantages of effectively removing the organic particles, inorganic nitrogen and phosphor and other nutritive salts and building excellent water quality, and can be applied to the freshwater fish closed cycling aquaculture system.

The invention relates to the technical field of feeds, and in particular relates to a goose feed containing Chinese medicinal additives. The feed is prepared from the following raw materials in percentage by weight: 50-60 percent of corns, 15-18 percent of rye bran, 12-15 percentage of bean pulp, 12-15 percent of fried soybean meal, 10-12 percentage of barley malt, 13-15 percent of dried brewer grains, 12-16 percent of sorghum meal, 3-4 percent of Italian ryegrass, 2-3 percent of egg shell meal, 1-2 percent of gravel, 3-4 percent of potato stem, 2-3 percent of earthworm dry powder, 1-2 percent of Chinese medicinal additives, a proper amount of table salt and 0.2-0.5 percent of a phagostimulant. The goose feed containing the Chinese medicinal additives provided by the invention is rich in the types of raw materials and is sufficient in nutrition; Chinese herbal medicines such as sargentg loryvine stem, giant knotweed, mulberry and the like can improve the growing performance of geese, increase the weight gaining speed, increase the utilization ratio of the feed and effectively reduce the feed-to-gain ratio.

The invention discloses a method for planting polygonum multiflorum. The method comprises the steps as follows: selecting land, raising seedlings by cutting, watering, applying fertilizer and racking. In the step of raising the seedlings by cutting, the plant spacing is 3 cm, the row distance is 10 cm, and upper sections of cuttage branches are exposed out of the earth surface by 3 cm after earthing. According to the method, the plant spacing is defined to be 3 cm, the row distance is defined to be 10 cm, and the upper sections of the cuttage branches are required to be exposed out of the earth surface by 3 cm after earthing in the step of raising the seedlings by cutting, so that not only can the polygonum multiflorum completely absorb nutrients, water and sunlight, but also land resources, water resources and fertilizers and the like can be saved to the greatest extent, the planting cost is reduced and the yield is increased at the same time. According to the statistics, after the method is adopted, the dry weight of the yield per mu (1 mu is 1 / 6 of acre) of the planted polygonum multiflorum can reach 2200-2600 kg / mu, and the yield per mu of the planted polygonum multiflorum is increased by 30% relative to that of the conventional planted polygonum multiflorum, so that the method is suitable for large-scale planting.

The invention relates to a weeding composition containing florasulam and clodinafop-propargyl, a preparation method and use thereof, wherein, the weeding composition is composed of a compound (A)-the florasulam and a compound (B)-the clodinafop-propargyl. The weeding composition of the invention obtained based on a proper mixing ratio can be processed into conventional formulations of pesticide such as wettable powder, suspension concentrate, water dispersible granules and the like by adopting a modern pesticide preparation processing technology, and the weeding composition can be used for controlling gramineous weeds and broadleaf weeds in fields such as cleavers, lithospermum arvense, alisma rhizome, common vetch, chickweed, capsella, silene jenisseensis willd, chorispora tenella, pennsylvania bittercress, stellaria alsine grimm, polygonum convolvulus, salvia alopecurus aequalis, wild oat, animated oat, brachiaria, crabgrass, barnyard grass, goosegrass, Italian ryegrass, goosefoot, common annual bluegrass, setaria glauca, green bristlegrass herb, Johnson grass and the like. The weeding composition has obvious control effect compared with the mode of only using the compound (A) or the compound (B) and significantly reduces application dosage of each active substance; and the weeding composition has great ecological and environmental protection significance.

The invention discloses a pest control method for efficient cultivation of polygonum multiflorum, which comprises agricultural control, physical control, biological control and chemical control. The agricultural control comprises the following steps: in summer and autumn, after harvesting the last batch of crops, deep ploughing and drying upturned soil in time, exposing and drying the soil, wherein the thickness of deep ploughing is 25-30 cm, and the exposing and drying time is 30 days. By the method that in summer and winter, after harvesting the last batch of crops, the upturned soil is deeply ploughed and dried in time, and the soil is exposed and dried, wherein the thickness of deep ploughing is 25-30 cm, and the exposing and drying time is 30 days, germs invading polygonum multiflorum plants can be conveniently controlled; and thus the control efficiency is improved. The ploughing depth can reduce requirements to the ploughing tools and machines. In addition, the exposing and drying time is limited to be 30 days, which not only cannot enable the nutritional components in the soil to be lost, but also can improve the physical behaviors of the soil, reduce influence of the pest and disease damage to polygonum multiflorum, ensure the emergence rate of polygonum multiflorum, and improve the output of polygonum multiflorum.

The invention discloses a polygonum multiflorum cultivating method and belongs to the technical field of plant cultivation. The polygonum multiflorum cultivating method includes steps of selecting and preparing land soil, selecting cuttage twigs with 2-4 knots, soaking rooting agent and cutting the cuttage, fertilizing respectively when seedlings grow to the next May and the next September, putting up a support when the seedlings grow to certain height, spraying 800-times solution of 50% of thiophanate wettable powder and 800-1000-times solution of 50% of carbendazim wettable powder when plants have leaf spot diseases; spraying 800-1000-times solution of 70% of thiophanate wettable powder to stems when the roots have root rot; harvesting finally. By the polygonum multiflorum cultivating method, yield of polygonum multiflorum is remarkably increased to 1200-1500kg / mu (dry weight). Besides, the polygonum multiflorum cultivating method is simple and low in production cost.

The invention relates to a quality control method of polygonum multiflorum-Alpinia oxyphylla capsules, in particular to a fingerprint spectrum detection method of polygonum multiflorum-Alpinia oxyphylla capsules, and belongs to the technical field of medicine analysis. The fingerprint spectrum detection method includes the steps of firstly, preparing a control solution; secondly, preparing an internal standard solution; thirdly, preparing a test solution; fourthly, using high performance liquid chromatography to perform analysis and detection. The fingerprint spectrum detection method has the advantages that an internal standard peak is innovatively quantitatively added into the complex fingerprint spectrum of traditional Chinese medicine preparations so as to calibrate the chromatographic peaks in the preparations, and the method is accurate, simple, high in sensitivity and good in reproducibility; the method can comprehensively monitor the quality of semi-finished and finished products and the stability of production process.

The invention belongs to the technical field of pesticides and particularly relates to a synergistic weeding composition for preventing and controlling herbicide-resistant weeds in wheat fields. The synergistic weeding composition includes an active component and an active component B, wherein the active component A is bis carfentrazone, the active ingredient B is an isoxazole compound fenoxasulfone. The composition is sprayed to stems and leaves before a four-leaf stage after weeds emerge from the ground, can effectively prevent and control descurainia sophia, shepherd's purse, lithospermum arvense and other broad-leaf weeds and alopecurus aequalis Sobol, Italian ryegrass, bromegrass and other grassy weeds, is remarkable in synergistic effect and also has the remarkable preventing and killing effect on the weeds resistant to ALS inhibitor type herbicides and ACCase inhibitor type herbicides after being compounded. The synergistic weeding composition is lower in compounding cost, simple to apply, remarkable in synergistic preventing and killing effect and convenient to popularize and has important economic and social values.

The invention discloses duck feed containing lolium multiflorum. The duck feed consists of the lolium multiflorum and conventional duck feed and comprises ingredients (A), ingredients (B) and ingredients (C), wherein the weight ratio of the lolium multiflorum to the conventional duck feed of the ingredients (A) is 1:0, the weight ratio of the lolium multiflorum to the conventional duck feed of the ingredients (B) is 1:1, and the weight ratio of the lolium multiflorum to the conventional duck feed of the ingredients (C) is 1:2. The duck feed containing the lolium multiflorum is adopted for feeding rice field culture ducks for 1 to 9 weeks, wherein the ingredients (A) are used in the first to three weeks, the ingredients (B) are used in the fourth to sixth weeks, and the ingredients (C) areused in the seventh to ninth weeks. The experience for utilizing the lolium multiflorum as dynamic alternative feed for realizing ecological culture of ducks in the rice field is successfully summarized, the feed cost reduction is realized, the fat content of the duck meat is obviously reduced, and safer and more environment-friendly duck products can be ensured to be produced.

The invention provides a grazing and supplementary feeding combined beef cattle breeding method. The method is characterized by adopting the way of combining supplementary feeding and grazing, prolonging the grazing time during summer and autumn with no or little supplementation of feed, and additionally and substantially supplementing feed during spring and winter. The feed comprises green feed and mixed feed, wherein the green feed refers to various herbage at grassy hills and slopes, and consists of crop straw, coarse cereals, artificially planted herbage like Lolium multiflorum, Purus frumentum, sorghum sudan grass, medicago sativa, and Trifolium repens, etc., and the mixed feed is proportioned according to the needs of the beef cattle feeding stage. The breeding method provided by the invention combines grazing and supplementary feeding, and has the characteristics of high meat-production rate and high dressing percentage.

The invention provides a method for culturing italian ryegrass, and belongs to the technical field of plantation, and especially relates to a culture technique for ryegrass. The invention provides the method for culturing italian ryegrass, and the method is easy in planting, convenient in management, high in yield, and easy in harvesting. The method for culturing italian ryegrass is characterized by comprising the following steps: 1, in sowing time, italian ryegrass Hercules can be sown in spring and autumn, combining with rain or irrigation, sowing after rain can easily obtain full stand; 2, seeding rate of the italian ryegrass Hercules being related to local natural conditions, soil conditions, sowing methods, and utilization objectives; 3, sowing method including: (1) sowing in drill, specification being 15-30 cm of line spacing and 5-10 cm of sowing width, (2) performing broadcast sowing, broadcast sowing being a most commonly used simple sowing method for farmers; 4, irrigating and draining, the italian ryegrass Hercules preferring wet and cannot stand waterlogging; 5, applying fertilizer, according to seedling conditions, and dressing a nitrogenous fertilizer once respectively in a trefoil stage and a tillering stage after seedlings emerge.

The invention mainly relates to the technical field of planting, and discloses a seed treatment method for accelerating germination of Italian ryegrass. The seed treatment method includes seed immersion, low-temperature storage, seed sprouting and seed sowing. The method is simple and seeds can be treated in batches; the method can obviously accelerate the seed germination of Italian ryegrass so that the germination time is shortened to 3-4 days, the planting efficiency is improved and the planting cost is saved; through immersing Italian ryegrass seeds in high-temperature acetate solution, the seed coat softening is accelerated and nutrition components are promoted to enter the seeds; low pH value can stimulate the enhancement of embryo activity, accelerate seed germination and shorten the germination time; after immersing, the seed is added with seed-dressing agent to carry out low-temperature storage; for the seed-dressing agent is rich in multiple nutrition and antibiotic constituents, the seed absorption is promoted, and the seed stress resistance is improved; moreover, the seed germination is stimulated under a low-temperature and dim-light environment, the germination rate is improved to 98.6%, and thus the planting cost is saved.

The invention discloses a measuring method for quickly measuring content of soluble sugar in lolium multiflorum. The measuring method comprises the following steps of providing a predicting model for predicting actual content of soluble sugar in the lolium multiflorum based on an near-infrared spectrum technique; on the basis of known actual contents of more samples, collecting the near-infrared original spectrum of each sample, and establishing a quantitative analysis predicting model for predicting content of soluble sugar in the lolium multiflorum based on the near-infrared spectrum technique and a chemometrics method, and pretreating a to-be-measured lolium multiflorum sample, so as to obtain to-be-measured lolium multiflorum sample powder; collecting the near-infrared original spectrogram, and detecting after the near-infrared spectrum is collected, wherein the spectrum collection process time is short. The measuring method has the characteristics that the operation is simple, the detection is rapid, the detection efficiency is high, the detection accuracy is high, and the like; the measuring method is suitable for being popularized and applied to the field of nutrient quality evaluation of grass.

The invention discloses a method for combining breeding and breeding of chickens under a grape trellis. The invention fully utilizes the land and space of grapes and maximizes resource utilization. Grass is planted in the vineyard, which makes the grass a dominant group and inhibits the growth of weeds, thus reducing the cost of pest control and pesticide pollution in the vineyard. The selection of suitable forage varieties and the amount of chickens can exert the allelopathic effect of plants to promote the growth of grapes. At the same time, raising chickens provides organic fertilizer for grapes, ensuring the supply of organic nutrients and solving the problem of fertilizer competition. Rotational grazing and recovery period management techniques are used to ensure the growth of pasture and avoid surface soil compaction. Four kinds of pastures, such as ryegrass multiflora, bitter weed, chicory and amaranth, are selected as the main pastures in the vineyard, which ensures a balanced supply of pastures throughout the year.

The present invention provides a tissue culture fast-propagation method of Chinese medicinal plant polygonum multiflorum. Said method mainly includes the following several steps: preparing explant, induction culture, differentiation culture and rhizogenetic culture. Besides, said invention also provides the concrete requirements of the above-mentioned every step and its concrete operation method.

The invention discloses a method for rapidly determining the content of crude protein in lolium multiflorum. According to the determining method, a prediction model, based on the near infrared spectrum technology, about the real content of the crude protein in the lolium multiflorumis provided, on the basis that the real content of a large number of samples is known, the near infrared original spectrum of a sample is acquired, the quantitative analysis prediction model, based on the near infrared spectrum technology and a chemical measuring method, about the content of the crude protein of the lolium multiflorum is established, then, the to-be-determined lolium multiflorum sample is pretreated, and to-be-determined lolium multiflorum sample powder is obtained; a near infrared original spectrum chart is acquired, the time of the spectrum acquisition process is short, and detection can be performed after the near infrared spectrum is acquired. The method has the characteristics of being simple to operate, rapid in detection, high in detection efficiency, high in detection accuracy and the like and is suitable for popularization and application in the field of pasture nutrition quality evaluation.

The invention relates to the technical field of pesticides and particularly relates to synergistic weeding composition containing dicarfentrazone-ethyl and dinitraniline type compounds. The synergistic weeding composition comprises an active ingredient A and an active ingredient B which are effective for weed control, wherein the active ingredient A is a compound dicarfentrazone-ethyl, and the active ingredient B is one or more of dinitraniline type compounds, namely, pendimethalin, trifluralin and butralin. The synergistic weeding composition can be used for controlling broad leaved weeds such as descurainia sophia, shepherd's purse, lithospermum arvense and the like and grassy weeds such as alopecurus aequalis, lolium multiflorum, bromegrass and the like and has a remarkable control effect on resistant weeds.

The invention provides a weeding composition containing saflufenacil, glufosinate ammonium and quizalofop-p-ethyl. The weeding composition comprises the following active components saflufenacil, glufosinate ammonium and quizalofop-p-ethyl according to a mass ratio 1-50:0.1-20:0.1-20; the mass of the active components in the preparation is 1-60% of the total mass of the whole preparation, and the rest components are auxiliary components which are allowed to be used and accepted in pesticide; the dosage forms of the weeding composition comprise missible oil, suspending agent, wettable powder, water dispersible granule, emulsion in water, micron emulsion, particulate agent, microcapsule, and aqueous solution. The components can be used for effectively controlling main weeds in rice fields, such as barnyard grass, caper euphorbia seeds, crab grass, goosegrass herb and the like as well as winter wheat weeds, such as Alopecurus aequalis, Avena fatua, Polypogon fugax, Poa annua, Sclerochloa dura, Puccinellia distans, Lolium multiflorum, Lolium temulentum, Bromus japonicas, Phleum paniculatum, Aegilops tauschii, Beckmannia syzigachne, Mesembryanthemum crystallinum, shepherd's purse, Descurainia sophia, Malachium aquaticum, and the like.

The invention belongs to the technical field of feed, and in particular relates to cattle feed capable of improving meat quality, and a preparation method thereof. The cattle feed is prepared from Italian ryegrass, soybean straws, calcium bicarbonate, traditional Chinese medicine additives, trace element mixing materials, vitamin mixing materials and a condiment. The feed has the characteristics of high palatability, high nutrient content and easiness in absorption, and can improve the beef quality and improve the immunity of cattle.

The invention discloses a method for improving the quality and yield of Aubade floribunda ryegrass seeds, and the method comprises the following steps of: (1) selection of a breeding base; (2) treatment of soil before sowing; (3) treatment of seeds before sowing; (4) sowing; and (5) weeding, fertilization, pest elimination after sowing. The step (2) comprises the following steps of: a, ploughing, harrowing, and turning over the soil; b, planning and designing the field; c, unselectively eliminating pests; and d, applying basal fertilizers: applying 300kg / hm<2> of basal fertilizer after the step c, wherein the basal fertilizer is a compound fertilize, in which the masses of N, P and K are respectively 15% of the total mass of the basal fertilizer. In the step (3), the seeds are treated for removing beards of the seeds; and in the step (4), a sower is used for sowing, and the row space is 40-60cm. The Aubade floribunda ryegrass planted through the invention can produce more than 60kg / mu of grass seeds each year, and the yield is obviously higher than that yield of Aubade floribunda ryegrass planted through the traditional planting method. The quality of the treated grass seeds can reach the quality standard of the grass seeds in the I-II national level.

The invention relates to a method for determining regeneration consumption in-vivo carbohydrate of roots of leaf-removed lolium multiflorum. The method comprises the following steps of: taking 36-48 plants of growth consistent lolium multiflorum in two weeks, after growing the lolium multiflorum for eight weeks under the conditions of the temperature of 18-25 DEG C and illumination of 6-8 h everyday, respectively detecting the lolium multiflorum to obtain the average carbon content of roots of the lolium multiflorum before removing leaves, the average carbon content of crops of the lolium multiflorum before removing leaves, and cutting off the roots to culture by keeping out of the sun, detecting the total carbon content of the regeneration consumption in-vivo carbohydrate of the roots of the lolium multiflorum after removing leaves each time, and calculating the amount of the regeneration consumption carbohydrate of the roots of regenerated lolium multiflorum. The method for determining the regeneration consumption in-vivo carbohydrate of the roots of the leaf-removed lolium multiflorum provided by the invention has the advantages of being simple in determination method, simple for operation and increased in production efficiency and working efficiency.

Provided is a herbicidal composition which contains fluoroglycofen-ethyl, glyphosate and haloxyfop-p-methyl. A mass ratio of effective components including the fluoroglycofen-ethyl, the glyphosate and the haloxyfop-p-methyl in the herbicidal composition is 1-50:0.1-20:0.1-20; a total mass of the effective components in the composition accounts for 1%-60% of a total mass of the composition; the rest components are permitted and acceptable auxilairy components in pesticide; and the formulation form of the herbicidal composition can be emulsifiable concentrate, suspension concentrate, wettable powder, water dispersible granules, aqueous emulsion, microemulsion, granules, microcapsules or water aqua. Main weeds in rice fields, such as barnyard grass, leptochloa chinensis, digitaria sanguinalis and goosegrass herbs, and winter wheat weeds such as amur foxtail, avena fatua, polypogon fugax, bluegrass, sclerochloa dura, puccinellia tenuiflora, Italian ryegrass, lolium temulentum, bromegrass, phleum paniculatum huds, aegilops tauschii, beckmannia syzigachne, wheatgrass, shepherd's purse, descurainia sophia and crickweed, can be prevented and removed effectively.

The invention relates to a method for measuring stored carbohydrates capable of being utilized for regeneration of lolium multiflorum leaves after removing leaves. The method comprises the following steps of: taking 36-48 plants of lolium multiflorum which are consistent in growth and 3 years old, culturing for 7 weeks under the conditions that the temperature is 18-25 DEG C and the illumination time is 6-8 hours every day, respectively detecting the average carbon storage Cr in the root parts of the lolium multiflorum before removing the leaves, the average carbon storage Cs in stubbles of the lolium multiflorum before removing the leaves and the total average carbon storage Cn in regenerated leaf blades of the lolium multiflorum, and calculating the relative maximum amount RI of the stored carbohydrates capable of being utilized for regeneration of the lolium multiflorum leaves. According to the method for measuring the stored carbohydrates capable of being utilized for regenerationof the lolium multiflorum leaves, provided by the invention, the measuring method is simple, the relative maximum amount of the stored carbohydrates which can be utilized for regeneration of the leafblades of the lolium multiflorum before removing the leaves can be detected, the process is simple, the operation is simple, and the production efficiency and the working benefits can be further improved.

Provided is a herbicidal composition containing saflufenacil, glyphosate and fluazifop-p-butyl. A mass ratio of effective components including the saflufenacil, the glyphosate and the fluazifop-p-butyl in the herbicidal composition is 1-50:0.1-20:0.1-20; a total mass of the effective components in the composition accounts for 1%-60% of a total mass of the composition, and the rest components are permitted and acceptable auxilairy components in pesticide; and the formulation form of the herbicidal composition can be emulsifiable concentrate, suspension concentrate, wettable powder, water dispersible granules, aqueous emulsion, microemulsion, granules, microcapsules or water aqua. Main weeds in rice fields, such as barnyard grass, leptochloa chinensis, digitaria sanguinalis and goosegrass herbs, and winter wheat weeds such as amur foxtail, avena fatua, polypogon fugax, bluegrass, sclerochloa dura, puccinellia tenuiflora, Italian ryegrass, lolium temulentum, bromegrass, phleum paniculatum huds, aegilops tauschii, beckmannia syzigachne, wheatgrass, shepherd's purse, descurainia sophia and crickweed, can be prevented and removed effectively.

The invention discloses a herbicide composition containing metamifop, cyhalofop-butyl, and bentazone, and the balance auxiliary ingredients which are allowed to use or are acceptable in pesiticides. The mass ratio of active ingredient metamifop, cyhalofop-butyl, and bentazone in the herbicide composition is 1-50:0.1-20:0.1-40, the total mass amount of the active ingredients accounts for 1 to 6% of the total mass amount of the herbicide composition. The herbicide composition can be made into missible oil, suspending agents, wettable powder, water dispersible granules, emulsion in water, microemulsions, granules, microcapsules, and aqueous solutions. The herbicide composition can be used for preventing and killing paddy field main weeds, such as barnyard grass, euphorbiae lathyridis, crab grass, eleusine indica, and winter wheat weeds, such as amur foxtail, oat grass, polypogon fugax, bluegrass, sclerochloa dura, puccinellia distans parl, Italian ryegrass, lolium temulentum, bromegrass, phleum paniculatum huds, aegilops tauschii, beckmannia syzigachne, wheatgrass, capsella bursa-pastoris, flixweed, and malachium aquaticum, effectively.

The invention discloses a method for constructing a lolium multiflorum tissue culture regeneration system. The method can be used for providing a technical reference for the selection and breeding of excellent lolium multiflorum varieties. The method comprises the steps of adopting mature lolium multiflorum seeds as explants, cleaning and sterilizing the mature lolium multiflorum seeds, then, inoculating to a callus induction culture medium, culturing for 18-22 days so as to obtain a callus, then, transferring the callus into a callus subculture medium, culturing for 18-22 days, then, transferring to a callus differentiation culture medium, culturing for 18-22 days, transferring seedlings into a rooting culture medium when the seedlings grow to 2-3cm, and culturing for 20-30 days. The efficient lolium multiflorum tissue culture regeneration system is constructed by the method, so that a basis for constructing an efficient genetic transformation system of lolium multiflorum tissue is laid, and a technical reference for the selection and breeding of excellent lolium multiflorum tissue varieties is provided; the method is suitable for being popularized and applied to the field of biotechnologies.

The invention belongs to the field of medical botany and a technology for planting plants and specifically relates to a technology for cultivating Polygonum multiflorum by pruning roots. The technology is composed of five main steps of collecting and treating seeds, sowing and culturing seedlings, lifting, pruning roots and transplanting; the specific operation method comprises the following steps of: collecting and treating seeds; collecting fruits when the green Polygonum multiflorum fruits become greenish yellow in every year; putting the fruits in a room to be dried in the shade; removing fruit stems, fruit peels and other impurities; and removing damaged seeds and shrunken seeds. The technology provided by the invention has the advantages of simplicity in operation and strong practical applicability, is suitable for popularizing planting in a large area, improves the survival rate of the artificially planted Polygonum multiflorum, and greatly improves yield and quality of the artificially planted Polygonum multiflorum.

The invention discloses a cuttage reproduction method of polygonum multiflorum. According to the cuttage reproduction method, lignified twigs of polygonum multiflorum which healthy grows for half a year to two years are taken as cutting slips, the cutting slips are specially processed, fertilizers are reasonably screened, and a proper cottage method is utilized, so that the survival rate of the cut polygonum multiflorum exceeds 95%, and high-quality seedlings can be provided for the field production of the polygonum multiflorum; and furthermore, the whole process is simple, the operation is simple, the breeding cycle is short, and the polygonum multiflorum can be transplanted after 30-45 days. The cuttage reproduction method is completely applicable to field cultivation.