Method for constructing lolium multiflorum tissue culture regeneration system

A technology of ryegrass multiflora and tissue culture, which is applied in the field of establishment of tissue culture regeneration system of ryegras multiflora, can solve the problem of affecting the yield and quality of ryegras multiflora, and provide technical reference for selection of fine varieties of ryegras multiflora. Problems such as transgenic technology have not been seen yet, and the effect of increasing induction rate, strong root system, and increasing differentiation rate has been achieved

Inactive Publication Date: 2014-08-13
SICHUAN AGRI UNIV
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  • Claims
  • Application Information

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Problems solved by technology

However, due to the strong competition between field weeds and Lolium multiflora, the yield and quality of Lolium multiflora are seriously affected.
In order to improve the removal efficiency of ryegrass multiflora in the field and reduce the cost of forage production, it is necessary to breed excellent varieties of ryegrass multiflora to improve the competitiveness of ryegrass multiflora. Research on cultivation regeneration system and transgenic technology cannot provide technical reference for breeding fine varieties of Lolium multiflora

Method used

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  • Method for constructing lolium multiflorum tissue culture regeneration system
  • Method for constructing lolium multiflorum tissue culture regeneration system
  • Method for constructing lolium multiflorum tissue culture regeneration system

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Embodiment Construction

[0020] The present invention adopts mature ryegrass multiflora seeds as explants, cleans and sterilizes mature ryegrass multiflora seeds, inoculates them into callus induction medium and cultivates them for 18-22 days to obtain callus tissue, which is prepared by MS medium, 1~20 days. The callus induction medium composed of 5mg / L 2,4-D and 0.5~3mg / L 6-BA can greatly increase the induction rate, and then transfer the callus to the callus subculture medium for 18 ~22d, the callus subculture medium composed of MS medium, 1~3mg / L 2,4-D and 0~0.3mg / L 6-BA can make the callus proliferate fastest and have good quality , and then transfer it to callus differentiation medium for 18-22 days. The callus differentiation medium composed of MS medium and 0.5-2 mg / L 6-BA can greatly increase the differentiation rate. When the seedlings grow When the seedlings reach 2-3cm, put the seedlings into the rooting medium and cultivate them for 20-30d. The rooting medium composed of 1 / 2MS medium can gr...

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Abstract

The invention discloses a method for constructing a lolium multiflorum tissue culture regeneration system. The method can be used for providing a technical reference for the selection and breeding of excellent lolium multiflorum varieties. The method comprises the steps of adopting mature lolium multiflorum seeds as explants, cleaning and sterilizing the mature lolium multiflorum seeds, then, inoculating to a callus induction culture medium, culturing for 18-22 days so as to obtain a callus, then, transferring the callus into a callus subculture medium, culturing for 18-22 days, then, transferring to a callus differentiation culture medium, culturing for 18-22 days, transferring seedlings into a rooting culture medium when the seedlings grow to 2-3cm, and culturing for 20-30 days. The efficient lolium multiflorum tissue culture regeneration system is constructed by the method, so that a basis for constructing an efficient genetic transformation system of lolium multiflorum tissue is laid, and a technical reference for the selection and breeding of excellent lolium multiflorum tissue varieties is provided; the method is suitable for being popularized and applied to the field of biotechnologies.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for establishing a tissue culture regeneration system of Lolium multiflora. Background technique [0002] Multiflora ryegrass is an annual or perennial plant with dense and thin fibrous roots, many stalks, and an erect height of 50-70 cm. Spikes flat. Multiflora ryegrass has good palatability, and all kinds of livestock like to eat it. Multiflora ryegrass is suitable for cutting green feed, making high-quality hay, and can also be used for grazing. It is also a good bait for fish farming. In southern my country, it is often planted beside fish ponds to feed grass carp. Multiflora ryegrass is of good quality and rich in protein. In the foliage stage, due to fewer stems and more leaves, the quality is better. Lolium multiflora is an important annual or short-lived perennial grass forage, suitable for winter and spring crops in field rotation. [0003] Lolium mu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 杨春华刘刚睢艺芳余克非李达旭孙飞达刘琳杨勇
Owner SICHUAN AGRI UNIV
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