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Culture medium of induced haplobiont for culturing eggplant anther and method of culture medium

An eggplant flower and culture medium technology, applied in the field of plant biology, can solve the problems of low doubling rate of haploid plants, low callus induction rate, low differentiation rate of regenerated plants, etc. The effect of long cultivation time

Inactive Publication Date: 2015-04-29
郑州市蔬菜研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the shortcomings of the application of eggplant anther culture in actual breeding include low callus induction rate, low differentiation rate of regenerated plants, low doubling rate of haploid plants, long culture period, etc.

Method used

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  • Culture medium of induced haplobiont for culturing eggplant anther and method of culture medium
  • Culture medium of induced haplobiont for culturing eggplant anther and method of culture medium
  • Culture medium of induced haplobiont for culturing eggplant anther and method of culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: a kind of culture medium and method thereof of eggplant anther culture induction haploid plant, its steps are as follows:

[0030] 1) Select the flower buds from the position of the eggplant plant growing on the eggplant or four-door eggplant. The anther cells in the flower buds are in the middle stage of mononuclei to the marginal stage of mononuclei. The diameter of the flower buds is 10mm, and the anthers are yellow-green to greenish;

[0031] 2) Pretreatment: Seal the collected flower buds with a waterproof bag or a culture bottle, and store them in a refrigerator at 4-5°C for 24 hours;

[0032] 3) Sterilization: first use 75% alcohol to disinfect the surface of flower buds for 30 seconds, then soak for 20 minutes in a solution of 7% sodium hypochlorite stock solution, in which the mass concentration of sodium hypochlorite in the stock solution of sodium hypochlorite is 10%, and then wash with sterile water 4 times , 3 minutes each time, and then dr...

Embodiment 2

[0043] Embodiment 2: a kind of culture medium and method thereof of eggplant anther culture induction haploid plant, its steps are as follows:

[0044] 1) Select the flower buds from the position of the eggplant plant growing on the eggplant or four-door eggplant. The anther cells in the flower buds are in the middle stage of mononuclei to the marginal stage of mononuclei. The diameter of the flower buds is 10mm, and the anthers are yellow-green to greenish;

[0045] 2) Pretreatment: Seal the collected flower buds with a waterproof bag or a culture bottle, and store them in a refrigerator at 4-5°C for 24 hours;

[0046] 3) Sterilization: first use 75% alcohol to disinfect the surface of flower buds for 30 seconds, then soak for 20 minutes in a solution of 7% sodium hypochlorite stock solution, in which the mass concentration of sodium hypochlorite in the stock solution of sodium hypochlorite is 10%, and then wash with sterile water 4 times , 3 minutes each time, and then dry t...

Embodiment 3

[0057] Embodiment 3: a kind of culture medium and method thereof of eggplant anther culture induction haploid plant, its steps are as follows:

[0058] 1) Select the flower buds from the position of the eggplant plant growing on the eggplant or four-door eggplant. The anther cells in the flower buds are in the middle stage of mononuclei to the marginal stage of mononuclei. The diameter of the flower buds is 10mm, and the anthers are yellow-green to greenish;

[0059] 2) Pretreatment: Seal the collected flower buds with a waterproof bag or a culture bottle, and store them in a refrigerator at 4-5°C for 24 hours;

[0060] 3) Sterilization: first use 70% alcohol to disinfect the surface of flower buds for 30 seconds, then soak for 20 minutes in a solution of 7% sodium hypochlorite stock solution, in which the mass concentration of sodium hypochlorite in the stock solution of sodium hypochlorite is 10%, and then wash 4 times with sterile water , 3 minutes each time, and then dry t...

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Abstract

The invention relates to a culture medium of an induced haplobiont for culturing eggplant anther and a method of the culture medium, and belongs to the technical field of plant biology. The method for cultivating the eggplant anther provided by the invention comprises the following steps: with the eggplant anther as a material, carrying out anther culture to induce callus; carrying out differentiation culture on the callus; carrying out rooting culture on differentiated seedlings; carrying out acclimatization and transplanting; and carrying out haplobiont doubling germination, so as to finish regeneration of an eggplant plant. The culture medium provided by the invention is low in cost and good in culture effect; the method for culturing the eggplant anther by virtue of the culture medium provided by the invention has the advantages of high callus induction rate, simple culture method and short culture period; the culture medium and the eggplant anther culture technology provided by the invention are applied to eggplant genetic breeding; the excellent genotype individual bodies of the eggplant are greatly enriched; the breeding period is obviously shortened; and a reliable technical support is provided for variety improvement of the eggplant.

Description

technical field [0001] The invention relates to a medium for cultivating eggplant anthers to induce haploid plants and a method thereof in the field of plant biotechnology. Background technique [0002] Plant anther culture technology has broad application prospects in the theoretical and practical research of crop genetics and breeding. Homozygous double haploids can be obtained quickly and effectively through anther culture, avoiding the need for multiple generations of selfing to obtain homozygous haploids through traditional breeding methods. Stable strains, thus greatly shortening the breeding cycle, improving selection efficiency and reducing costs. In addition, the double haploids obtained through anther culture can be directly used in the construction of genetic maps, breeding new varieties or used as excellent parents for variety improvement. The use of anther culture to induce haploid or double haploid has been successfully reported in many plants. At the s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李建欣田朝辉葛桂民庞淑敏申爱民赵香梅
Owner 郑州市蔬菜研究所
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