85results about How to "Extend incubation time" patented technology

The invention discloses an application of protein acetylation enzyme SIRT1 (Silent Mating type Information Regulation 2Homolog1), namely promoting proliferation and delaying senility of mesenchymal stem cells. The experimental result shows that the SIRT1 is needed by the in vitro proliferation of the mescenchymal stem cells, and the condition is verified by MSC (mesenchymal stem cells from marrow) and MSC from fat, and the proliferation and the senility of the MSC can be regulated by regulating the expression level of SIRT1 in the MSC. The invention has very important significances on the determination of the regulation mechanism of the proliferation and the senility of the MSC, the prolonging of the service life of the MSC, and the histocyte substitution and repairing treatment based on the MSC, and is broad in application prospect.

The invention relates to a culture medium of an induced haplobiont for culturing eggplant anther and a method of the culture medium, and belongs to the technical field of plant biology. The method for cultivating the eggplant anther provided by the invention comprises the following steps: with the eggplant anther as a material, carrying out anther culture to induce callus; carrying out differentiation culture on the callus; carrying out rooting culture on differentiated seedlings; carrying out acclimatization and transplanting; and carrying out haplobiont doubling germination, so as to finish regeneration of an eggplant plant. The culture medium provided by the invention is low in cost and good in culture effect; the method for culturing the eggplant anther by virtue of the culture medium provided by the invention has the advantages of high callus induction rate, simple culture method and short culture period; the culture medium and the eggplant anther culture technology provided by the invention are applied to eggplant genetic breeding; the excellent genotype individual bodies of the eggplant are greatly enriched; the breeding period is obviously shortened; and a reliable technical support is provided for variety improvement of the eggplant.

The invention provides an application of a compound microorganism bacterium agent in treatment of petrochemical sewage and sludge. The application comprises the following steps: (1) preparing a biological carrier, to be specific, adding glutaraldehyde to polyphenylene oxide, suspending, stirring, washing, then adding a PBS (Phosphate Buffer Solution) containing CaCl2 and standing; (2) carrying out enlarging cultivation of the compound microorganism bacterium agent; (3) putting the compound microorganism bacterium agent subjected to enlarging cultivation, obtained in the step (2), in the biological carrier prepared in the step (1); and (4) adding the biological carrier loaded with the compound microorganism bacterium agent, obtained in the step (3), to the petrochemical sewage sludge, wherein the compound microorganism bacterium agent continuously increases to a stable period and forms a biological membrane on the surface of the biological carrier, and the biological membrane metabolizes, adsorbs, absorbs, digests and resolves organic pollutants and heavy metals in petrochemical sewage and sludge, so that the petrochemical sewage and sludge are converted into stable harmless materials. Through research, the applicant finds that the compound microorganism bacterium agent can be used for treating the petrochemical sewage and sludge very well.

The invention discloses a method for reprogramming umbilical cord mesenchymal stem cells into liver cells. The method comprises the following steps of obtaining umbilical cord mesenchymal stem cells;carrying out pretreatment on the umbilical cord mesenchymal stem cells for two days; carrying out liver formation induction on the pretreated umbilical cord mesenchymal stem cells for seven days by using a liver formation induction composition; carrying out primary maturation induction on the umbilical cord mesenchymal stem cells subjected to liver formation induction treatment by using a first maturation induction composition for seven days to obtain liver cells; and carrying out second maturation induction on the liver cells for 12-20 days by using a second maturation induction composition to obtain the functional liver cells. Liver organoids comprise acellular matrix hydrogel, stem cells and liver cells, wherein the stem cells and liver cells being distributed in the acellular matrix hydrogel. According to the reprogramming method, the step of secondary maturation induction is added, so tat the culture time is prolonged, and the induction efficiency is remarkably improved. The liverorganoids provided by the invention have ultra-low immunogenicity and the possibility of cross-species transplantation.

The purpose is to provide the truffle mycelium and fruiting body cultivated by the medium that are developed for cultivating the truffles with a very high radical oxygen scavenging capacity. A medium for cultivating truffles includes a mixture of at least one carbohydrate source selected from a group consisting of rice bran, wheat bran, and glucose, and at least one edible ingredient selected from a group consisting of burdock, nuts, and berries. A weight ratio of the edible ingredient to the carbohydrate source is between 0.5 and 5. As for one of the ingredients, it is preferable that the burdock is granulated. The medium may exist in a liquid state. By injecting truffle seeds for cultivation into such a medium, the truffle mycelium and fruiting body with the high reactive oxygen scavenging capacity could be provided.

The invention provides an isolation and culture method for primary rat colonic smooth muscle cells. With the isolation and culture method provided by the invention, the acquired cells are great in gross quantity and high in both isolation degree and motility rate; the probability of contamination by bacteria, fungi and other cells is low; and the method can realize subculturing, so a good cell culture scheme is provided for related research based on culture of rat colonic smooth muscle cells.

The invention discloses a culture medium for culturing astroglia cells in vitro and a culture method and belongs to the field of cell culture. According to the culture medium, an epidermal growth factor, a fibroblast growth factor, SAG and Purmorphamine are added into an astroglia cell culture medium, so that the in-vitro culture time of the astroglia cells can be remarkably prolonged, the numberof time of passage is increased, the purity is improved and original properties of the astroglia cells can be kept. The inventor researches and finds out that the culture medium disclosed by the invention can be used across generations, and the astroglia cells are passed to 1 to 3 generations. The purity of the astroglia cells, which are cultured to 12 generations, is greater than 95 percent and the original properties of the astroglia cells can be kept.

The invention relates to the use of an agent such as an antibody capable of reacting with PrP in the prevention of prion replication in a subject, in the treatment or prevention of prion infection, in the treatment or prevention of neuropathology associated with prion infection or in the preparation of a medicament for the treatment or prevention of prion disease. Furthermore, the invention relates to methods of treatment of prion disease, methods of inhibiting prion replication and antibodies for use in such methods.

The invention belongs to the technical field of condiments, and particularly relates to a preparation method of calcareous black bean soy sauce. The calcareous black bean soy sauce is prepared from main raw materials as follows: black beans, pig elbow bone, wheat and coarse bran, wherein a ratio of the black beans, the pig elbow bone, the wheat and the coarse bran in parts by weight is (4-12):(2-6):(1-3):(1-4.5), and preferably, the ratio is 4:2:1:1.5. The preparation method of the calcareous black bean soy sauce comprises steps as follows: raw material processing; starter-making based on circulating ventilation; first solid fermentation and later liquid fermentation; oil sprinkling and sterilization; packaging, testing and storage; packaging and sampling test. Technological parameters are strictly controlled in the whole soy sauce preparation process, and are different from those just set in a certain large range for soy sauce on sale in the market. The calcareous black bean soy sauce prepared with the method is delicious in taste and has the function of calcium supplementation.

The invention discloses stem cell culture equipment. The stem cell culture equipment comprises a culture box; the front surface of the culture box is provided with a sealing cover; two clamping grooves are formed in the front surface of the culture box; the inner side of the sealing cover is fixedly connected with two fasteners; an installation box is fixedly installed in the culture box; the inner bottom part of the installation box is fixedly provided with a motor; the inner bottom part of the installation box is fixedly provided with a fixed frame body; an output end of the motor penetratesthrough one side of the fixed frame body and is fixedly connected with a first bevel gear; and the outer surface of the first bevel gear is in engaged connection with a second bevel gear. A culture dish is placed in a fixed holding groove; the culture dish can rotate along when a second rotating disc rotates, so that a culture solution in the culture dish is in a circular motion state, thus preventing the culture solution from being solidified when being statically placed, so that stem cells are kept active for a long time, and then the culture time is prolonged.

A Petri dish includes a receptacle and a matching lid, which both have a shape of revolution and are each delimited by a bottom wall and at least one peripheral rim. This also includes fingers and complementary locking elements. The receptacle and the lid, when rotated relative to each other, are able to occupy three or four different positions, including at least a “first position” and an “intermediate position”. This dish is distinguished by the fact that the locking elements carry at least one blocking member which blocks the fingers in the “intermediate position” and which opposes the return to the “first position”.

The invention discloses a preparation method of liver organoid. The preparation method comprises the following steps: uniformly mixing acellular matrix hydrogel, stem cells and liver cells by using a serum-free culture medium; conducting stationary culture in a culture container until the acellular matrix hydrogel is solidified; and after it is determined that cells in the solidified acellular matrix hydrogel survive, continuing culture to obtain the liver organoid. The liver organoid prepared according to the method of the invention has ultralow immunogenicity and has the potential in cross-species transplantation.