Culture medium for culturing astroglia cells in vitro and culture method

Abstract

The invention discloses a culture medium for culturing astroglia cells in vitro and a culture method and belongs to the field of cell culture. According to the culture medium, an epidermal growth factor, a fibroblast growth factor, SAG and Purmorphamine are added into an astroglia cell culture medium, so that the in-vitro culture time of the astroglia cells can be remarkably prolonged, the numberof time of passage is increased, the purity is improved and original properties of the astroglia cells can be kept. The inventor researches and finds out that the culture medium disclosed by the invention can be used across generations, and the astroglia cells are passed to 1 to 3 generations. The purity of the astroglia cells, which are cultured to 12 generations, is greater than 95 percent and the original properties of the astroglia cells can be kept.

Description

technical field [0001] The invention belongs to the field of cell culture, and in particular relates to a culture medium and a culture method for culturing astrocytes in vitro. Background technique [0002] Astrocytes are a type of glial cells that spread throughout the central nervous system and engage in multiple complex functions. They exchange substances with neurons and play an indispensable role in the growth of neurons. The main basis for identifying astrocytes is that they can express a glial fibrillary acidic protein (GFAP). In the central nervous system, Reactive astrocytes respond to various injuries and eventually form glial scars. In biology, it is often used to judge the damage site of the central nervous system. Astrocytes participate in the blood-cerebrospinal fluid barrier, have a certain regulatory effect on the blood flow of the central nervous system, and then affect the activity of neurons; astrocytes connect with synapses and play an important role in...

AI Technical Summary

Problems solved by technology

However, this method has little effect on cells that have been passaged for many times, and cells that lose division activity during the passage process cannot regain division properties by adding dBcAMP

At the same time, the cost of this culture method is relatively high, and the operation process is complicated.

[0004] In summary, the current methods for culturing astrocytes are complex and the cells grow slowly. Generally, the purity of astrocytes is 75-95% after in vitro culture until the third passage.

Operations such as changing the medium many times during the experiment not only increase the cost of the experiment, but also are not conducive to the growth of cells in a relatively stable environment, increasing the possibility of bacterial contamination

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