Disclosed are an
ammonia-oxidizing bacterium screening and testing
reagent box, a screening and testing method and a bacterium sample separating method. The
ammonia-oxidizing bacterium screening and testing
reagent box comprises a bacterium strain enrichment tube,
ammonia-oxidizing
testing tubes 1, 2 and 3, bacterium strain
preservative liquid and color developers A and B. The screening and testing method includes inoculating bacterium strains to be detected to the ammonia-oxidizing
testing tubes 1, 2 and 3 to culture after enriching the bacterium strains to be detected via the bacterium strain enrichment tube, and storing enrichment bacterium liquid by the bacterium strain
preservative liquid simultaneously; adding the
color developer A and B after culturing of the ammonia-oxidizing
testing tubes and judging ammonia-oxidizing capability of the bacterium strains according to color development states. The bacterium sample
separation method includes culturing bacterium samples, diluting, applying and separating. The ammonia-oxidizing bacterium screening and testing
reagent box, the screening and testing method and the bacterium sample separating method has the advantages that effect of quickly screening and testing ammonia-oxidizing
bacteria in high
throughput is realized, one-stop operations of culturing, testing and preserving of the bacterium strains are realized.