Apparatus and method for in vitro storage and culture of organ

Abstract

The invention belongs to the field of biomedical engineering, and discloses an apparatus and a method for in vitro storage and culture of an organ. According to the present invention, the human body blood circulation system is simulated to provide the in vitro organ environment close to the in vivo organ environment; the in vitro organ environment comprises the constant temperature and the circulation system; the circulation system comprises the oxygenator, the pump, the pressure control device, the organ placement box, the dialysis device, the liquid storage chamber and the agent feeding device; through the pressure control device, the perfusion pressure and the sufficient perfusion amount are maintained when the organ is subjected to the perfusion with the perfusion liquid, such that theorgan can be store in vitro for a long time and can maintain the health, and the culture of the in vitro constructed organ and the collection of the organ secretions are achieved; and the apparatus for reasonably achieving the in vitro organ storing and culturing and the technical scheme thereof are provided.

Description

technical field [0001] The invention belongs to the field of biomedical engineering; discloses a device and method for preserving and cultivating organs in vitro; realizes long-term preservation of organs in vitro and keeps them healthy; at the same time realizes the cultivation and secretion of organs after constructing organs in vitro collection; provide a reasonable device and technical solution for organ preservation and culture in vitro. Background technique [0002] Organ preservation was initially preserved by cold storage, and now it has been tried to preserve by perfusion; however, when perfusion is used for preservation, due to improper control of simulated physiological circulation blood flow and supply pressure, the organ cannot be effectively extended. Save time. [0003] There are strict requirements on the flow of blood flowing through the organs and the pressure in the blood vessels, and the relationship between flow and pressure is not a positive relationsh...

AI Technical Summary

Problems solved by technology

[0002] Organ preservation was initially preserved by cold storage, and now it has been tried to preserve by perfusion; however, when perfusion is used for preservation, due to improper control of simulated physiological circulation blood flow and supply pressure, the organ cannot be effectively extended. save time

[0003] There are strict requirements on the flow of blood flowing through the organs and the pressure in the blood vessels, and the relationship between flow and pressure is not a positive relationship; the flow is large, the pressure may not be high; and the flow is small, the pressure may not be low; because of this, Simply using the pump source for flow perfusion can easily cause internal damage to the organ; even if the pressure at the inlet and outlet of the perfusate is monitored to adjust the flow rate, the health of the organ will decline due to the supply of nutrients to the organ , or even necrosis; when the internal vascular channel of the organ is blocked, the pressure formed by the perfusate flowing through the organ will gradually increase. If the flow is reduced to reduce the pressure, or the flow is maintained to increase the pressure, it will cause damage to the health of the organ. decline or even necrosis

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