Ammonia-oxidizing bacterium screening and testing reagent box, screening and testing method and bacterium sample separating method

Abstract

Disclosed are an ammonia-oxidizing bacterium screening and testing reagent box, a screening and testing method and a bacterium sample separating method. The ammonia-oxidizing bacterium screening and testing reagent box comprises a bacterium strain enrichment tube, ammonia-oxidizing testing tubes 1, 2 and 3, bacterium strain preservative liquid and color developers A and B. The screening and testing method includes inoculating bacterium strains to be detected to the ammonia-oxidizing testing tubes 1, 2 and 3 to culture after enriching the bacterium strains to be detected via the bacterium strain enrichment tube, and storing enrichment bacterium liquid by the bacterium strain preservative liquid simultaneously; adding the color developer A and B after culturing of the ammonia-oxidizing testing tubes and judging ammonia-oxidizing capability of the bacterium strains according to color development states. The bacterium sample separation method includes culturing bacterium samples, diluting, applying and separating. The ammonia-oxidizing bacterium screening and testing reagent box, the screening and testing method and the bacterium sample separating method has the advantages that effect of quickly screening and testing ammonia-oxidizing bacteria in high throughput is realized, one-stop operations of culturing, testing and preserving of the bacterium strains are realized.

Description

Technical field: [0001] The invention belongs to a bacterial screening and identification method and a kit, in particular to an ammonia oxidizing bacteria screening and identification kit, screening identification and bacterial sample separation method. Background technique: [0002] Ammonia nitrogen is one of the important substances that cause water pollution. At present, urban sewage contains a large amount of ammonia nitrogen. If it is directly discharged into the natural environment, it will cause serious environmental pollution, lead to eutrophication of rivers and lakes, and weaken the self-purification ability of water bodies. Biological denitrification is currently one of the most economical and effective methods for denitrification of wastewater, and the conversion of ammonia nitrogen depends on the action of nitrifying microorganisms. Biological denitrification of sewage includes nitrification stage and denitrification stage. The nitrification stage includes two...

AI Technical Summary

Problems solved by technology

At present, various methods for screening ammonia oxidizing bacteria have achieved good results, but the screening and identification of ammonia oxidizing bacteria are generally verified indirectly by using artificial culture medium, and there is no verification in the sewage environment or fitting the results with the sewage environment Compared

Moreover, the traditional verification method has the problems of heavy workload and low efficiency

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