Method for breeding corn inbred line by using hybridizing induction of haploids

Abstract

The invention discloses a method for breeding a corn inbred line by using the hybridizing induction of haploids, and belongs to the field of crop breeding methods. The method comprises the following steps: pollinating basic population or cenospecies according with breeding targets by using JASS31, and selecting aleurone layer top purple and purple-free embryonic tip labeled grains; naturally or artificially doubling the obtained material, and inbreding; and selecting non-separated head progeny rows realizing consistent progenies as a new inbred line. The method has a high haploid inductivity reaching above 13%, and has the advantages of obvious selection labeling, easy screening and high accuracy; and additionally, the JASS31 used in the invention has a moderate height, a long flowering time and a large pollen amount, and natural pollination induction of haploids can be carried out, so the artificial pollination can be avoided, labor saving is realized, and it is suitable for the large scale population operation. The method also has the characteristics of high breeding efficiency, simple operation and fast breeding speed.

Description

technical field [0001] The invention belongs to a method for crop breeding, in particular to a method for breeding maize inbred lines by using hybridization to induce haploid. Background technique [0002] Breeding is a cyclical and long-term work. It generally takes 10-15 years to breed a variety. Not only is the workload heavy, time-consuming and laborious, but it may also take too long. Finally, the results obtained often do not meet the requirements of the market. Therefore, shortening the breeding time and speeding up the breeding speed have always been the goals that breeders are striving for. For this reason, many methods have been proposed, such as using anthers or pollen to cultivate haploids, and then doubling them into inbred lines can greatly shorten the breeding time. Time was once regarded as a promising method, but practice has proved that it is difficult to be widely used in practice due to the difficulty of cultivating anthers, complicated technology, and l...

AI Technical Summary

Problems solved by technology

However, Jigaoyu Line 3 has various disadvantages to varying degrees: (1) The plants are short and cannot be used for natural pollination in independent fields to produce haploids

However, inducible material produced by hybridization may present other problems, such as: reduced haploid induction rate and altered expression of marker genes

The R1-nj marker gene (Nanda and Chase, 1966) (purple scutellum and purple aleurone crown) is widely used for haploid selection from dry seeds, however, expression of this gene in induced material produced by crosses Highly influenced by the maternal parent, sometimes identifying haplotypes can be difficult or impossible, especially if the maternal suppressor gene (C1-I) is present

(2) Grain markings are inconsistent with different female parent materials, which increases the difficulty of haploid grain selection