279results about How to "The instrument is simple" patented technology

An immune bead is made up of the magnetic carrier micro ball which combines at least one immune matching base. The micro ball is composed of the magnetic nm particle and the high molecular framework material which the core is the metal particle; the out of the core is high molecular framework, the out layer is the functional layer which can combines functional gene of different immune matching base. The manufacture method includes: the bead pretreatment, the bead activation, manufacture of the coupling antibody, closing the antibody with the confining liquid and purifying the immune bead. The detecting method is to detect the different things by the immunological response sandwich, the competition and the indirect method and set the control system on the testing board. The board is made up of the encrusting test paper, the coupling mat, the sample mat, the water suction mat, the coving film and the testing board outside calipers. It has the high sensitivity and accurate quantity; the regent is simple and cost low.

The invention discloses a preparation method for the silica sol with a high evenness degree, belonging to the technical field of electronic manufacturing and SiO2 sol preparation. Under an ultrasonic influence, a silica sol seed solution is prepared and a reaction material is added to the silica sol solution in batches; aqueous solution and metallic silicon powder that serve as basic catalysts are chosen as the reaction material for each batch, and the basic catalyst in each batch is 2-40% of the basic catalyst previously added into the silica sol seed solution, and the metallic silicon powder in each batch is 2-40% of the metallic silicon powder previously added in the silica sol seed solution. After the reaction finishes, the unreacted metallic silicon powder is removed, and the SiO2 sol is produced. The ultrasonic sound adopted for the invention ranges from 20 to 50 kHz. By adopting the invention, production costs can be reduced and product purity is improved. In addition, the method disclosed by the invention has the advantages of safe technical process, simple equipment, and is easy to operate as well as is economic and has high efficiency.

The invention relates to a device for preparing macromolecule nano-fibre, and a spinning method. The device comprises: a nozzle which is connected with a fibre spraying tube and is electrically connected with the anode or the cathode of a high-voltage generator, wherein, a conductive receiving board is electrically connected with the cathode or the anode of the high-voltage generator; a drain sleeve, the lower port of which is sealed together with the upper port of the fibre spraying tube; and a heating coil which is arranged outside the fibre spraying tube and enwraps the fibre spraying tube tightly, wherein, a temperature transmitter is positioned between the heating coil and the fibre spraying tube and is attached to the fibre spraying tube, and a temperature feedback controller is electrically connected with the temperature transmitter and the heating coil respectively. A signal which is measured by the temperature transmitter is received and the working of the heating coil is regulated and controlled, to lead the contained macromolecular solution to reach the preset temperature. In the spinning method, the macromolecular solution which is prepared by polymer material is contained in the fibre spraying tube, and is heated until the temperature reaches the preset temperature through the temperature feedback controller; pressure is loaded, the macromolecule nano-fibre flows out from the nozzle under the action of the electric field force and is collected on the receiving board.

The invention belongs to the technical field of fuel batteries, and relates to a PBI-based polysiloxane and phosphonic acid high-temperature proton exchange film and a preparation method thereof. The PBI-based polysiloxane and phosphonic acid high-temperature proton exchange film disclosed by the invention is prepared through the following method: dispersing polysiloxane and phosphonic acid in dimethylacetamide, and preparing to obtain a polysiloxane and phosphonic acid gel; then dissolving PBI in dimethylacetamide to obtain PBI polymer solution; then uniformly mixing the polysiloxane and phosphonic acid gel with the PBI polymer solution, then preparing by a tape-casting film-forming technique to obtain the PBI-based polysiloxane and phosphonic acid high-temperature proton exchange film. The doping amount of phosphonic acid is low and phosphonic acid is not liable to exude when the high-temperature proton exchange film prepared by the preparation method disclosed by the invention achieves a certain proton conductivity, and the high-temperature proton exchange film has a high proton conductivity under a high-temperature and low-humidity condition; moreover, the medium-temperature proton exchange film is good in flexibility, high in mechanical strength, and good in durability in a high-temperature fuel battery.

The invention discloses a method for preparing composite dephosphorus adsorbent by virtue of activated carbon fiber loaded with metal. Carbon fiber is used as a matrix which is loaded with a metallic compound. The method comprises the following steps of: a, mixing lanthanum ions into an iron salt for preparing a mixture solution of iron ions and lanthanum ions; b, putting the prepared solution under an ultrasound environment, additionally adding a radiation method, gradually adding a hydroxide solution into a metallic ion mixture solution until the pH reaches 9-10, and obtaining nano-particle suspension liquid of iron and lanthanum composite metal hydroxide; c, shearing activated carbon fiber into pieces, washing, dipping, filtering and drying; d, weighing the activated carbon fiber, adding the activated carbon fiber into the suspension liquid, processing by utilizing ultrasound waves, taking out solid matters, and washing for multiple times until the pH reaches 7; and e, drying the solid matters, obtaining an adsorbent material after cooling, and sealing the adsorbent material for storage. According to the invention, the iron and lanthanum composite metal hydroxide is in uniform particle size distribution on the carbon fiber matrix, has a high adsorption capacity and a low preparation cost and is suitable for industrial application.

The invention discloses a preparation method of a composite proton exchange membrane, comprising the following steps of: sulfonating, preparing a sulfonated polyether ether ketone solution; preparing polyaniline filter liquid, preparing membrane preparing liquid, casting to form a membrane, and the like. The composite membrane is prepared by using SPEEK (sulfonated polyether ether ketone) as a basal body and PANI (Polyaniline) and HPA (Heteropoly acid) as dopants, a material source is wide and does not need to be imported, a preparation process is simple, and used instruments are simple, thereby the cost of the membrane is reduced. A hydrogen bond formed by PANI and SPEEK is used for avoiding the swelling of the SPEEK and reducing methanol penetration, and the proton conductivity of the composite membrane is improved by the HPA. By changing the composition and the ratio between an alkaline polymer and a proton conductor, the novel PEM (proton exchange membrane) reaches optimum balance among high proton conductivity, low methanol penetration and low HPA dropout rate, the proton conductivity reaches 10-2S / cm, the methanol penetration rate of the composite proton exchange membrane is a half lower than that of an Nafion 117 membrane, and the HPA wastage rate does not reach 5 percent.

The invention provides a method for detecting mercury ions through a gold electrode modified by polythymidine DNA, which is characterized in that polythymidine DNA molecule is taken as a recognition element for mercury ions, the gold electrode modified by polythymidine DNA is prepared, then mercury ions in an aqueous solution is detected by the prepared gold electrode, wherein alkali base T in DNA molecule is capable of specifically binding with mercury ions to form a stable T-Hg<2+>-T structure. The intensity of an electrochemical signal is relative with the concentration of mercury ions in the solution, so that the concentration of mercury ions in the aqueous solution can be calculated. According to the invention, the method for detecting mercury ions has the advantages of no pollution on environment, good selectivity on mercury ions, eliminated interference of most metal ions, high sensitivity, simple required apparatus and low cost, and is suitable for being applicable to such humble situations as fields and the like.

The invention provides a human body anti-AQP4 autoantibodies detection material and a preparation method thereof. The preparation method comprises the steps of firstly acquiring M1 and M23 subtype AQP4 overall length target genes, dyeing into an HEK293 cell, and building an HEK293 / pCI-neo-M1-AQP4 cell and an HEK293 / pCI-neo-M23-AQP4 cell expressing M1 and M23 subtype AQP4; extracting an AQP4-cell membrane complex of the cells; finally curing the AQP4-cell membrane complex on a carrier, and obtaining the human body anti-AQP4 autoantibodies detection material. According to the human body anti-AQP4 autoantibodies detection material and the preparation method thereof provided by the invention, the extracted AQP4-cell membrane complex is used as a detection material, so that the intrinsic protein amount causing background coloration can be reduced; compared with a method for using the cell as the detection material for carrying out immunofluorescence detection, the detection sensitivity and the specificity are further increased, a detection result is easier and clear to judge, and the AQP4 autoantibodies of a patient can be simply, conveniently and quickly detected.

The invention relates to a detection method for portable rapid visual detection of a copper ion content, and the method comprises the following steps: a. preparing a simple circuit, generating a platinum electrode of -0.65 V; b. preparing 13-nm gold nanoparticles; c. centrifuging the gold nanoparticles obtained in step b, dispersing in secondary water; d. obtaining azide-functionalized gold nanoparticles; e. obtaining alkynyl-functionalized gold nanoparticles; f. mixing the functionalized gold nanoparticles prepared in step d and step e, adding a sample solution to be detected; g. putting the potentiostatic platinum electrode obtained in step a into the solution of step f, performing electrolysis within 10 min, observing the color change of the gold nanoparticles. The device of the invention is protable, can realize the rapid and visual detection of the copper ion content in the sample, has strong maneuverability, and has the advantage of simple apparatus.

The invention discloses a polyaniline / zinc oxide nano composite resistor type material sensor and a preparation method thereof. The sensor comprises a ceramic substrate, an interdigital gold electrode and a gas-sensing material which are arranged in sequence, wherein the gas-sensing material is formed by compositing nanoscale lamellar zinc oxide and nano polyaniline particles; due to large specific surface area caused by nanoscale of the material, the adsorption site of the sensitive material and gas is increased and more channels are provided, so as to promote the adsorption and diffusion of the detection gas in the gas sensor. Particularly, in the preparation process of the sensor, only water is used as a solvent, organic solvents or other additives do not need to be added to participate in reaction, thus being green and safe and having no environment pollution. The reaction temperature is relatively low, high temperature firing is not needed, energy can be saved, and the preparation method has the prominent characteristic of environment friendliness. The method for preparing the gas sensor, provided by the invention, is safe and efficient, and is simple in process and low in cost, thus being suitable for batch production.

The invention relates to measurement for organic substances in soil, in particular to a method for measuring organic substances in soil. The method comprises the following steps: using a digestion instrument to replace a heating device; using a digestion tube to replace a conical flask to receive samples and reagent solution; covering a small funnel with a bent part above the digestion tube when heating; timing from boiling of the solution; taking down the digestion tube after 6 to 8 minutes; cooling the digestion tube; and using sodium diphenylamine sulphonate as indicator to titrate residual K2Cr2O4. The method has the advantages of good repeatability, high reproducibility, simplicity and practicability.

The invention discloses an EMI noise source impedance equivalent parameter extraction method based on scattering parameters and an intelligent algorithm. The EMI noise source impedance equivalent parameter extraction method is based on a scattering parameter principle, adopts a vector network analyzer and two current probes, respectively measures transmission parameters and reflection parameters when in short circuit, loading standard resistance and loading noise source impedance, calculates to obtain the impedance of a measured noise source, carries out optimizing processing on amplitude-frequency and phase-frequency characteristics of the measured impedance by utilizing a GA algorithm in the intelligent algorithm to obtain equivalent RLC parameters of the impedance, and provides general expression of amplitude value and phase position of the impedance. The EMI noise source impedance equivalent parameter extraction method is simple in instrument, does not need to use an auxiliary module, reduces hardware error caused by auxiliary modules such as a signal source and a frequency spectrograph, estimates parameters by using the GA algorithm, avoids the problem that initial values of parameters need to be given when methods such as the Newton-Gauss method and Marquardt method are adopted, increases measuring precision, and provides an accurate reference basis for the design of an EMI filter.

The production method of off-axis paraboloid mirror includes the following steps: making mirror blank; using a metal rectangular mirror frame; placing the mirror blank into the mirror frame and closing gap; respectively making marks on the centre of one side of mirror frame and correspondent position of mirror blank; polishing initial spherical surface of mirror blank; setting detection optical circuit, regulating angle and position of rectangular mirror frame relatively to parallel optical beam, making mirror surface angle meet requirements for off-axis quantity; using knife-edge shadow method to detect mirror surface; according to the shadow picture of 2-D knofe-edge regrinding mirror surface, repeatedly detecting and regrinding until the shaow picture is uniform in light and shade, so that the required off-axis paraboloid mirror can be obtained.

The invention provides a method for detecting hydroxyl radicals. The method comprises the following steps of: in an aqueous solution, oxidizing night blue by hydroxyl radicals so as to ensure that the night blue fades; selectively absorbing visible light with a wavelength of 618nm; and obtaining the concentration of the hydroxyl radicals in the solution through measuring the absorbancy of the solution. The method can detect the hydroxyl radicals within a concentration range of 0.039-0.379mg / L, has the advantages of simple instrument, low detecting cost, simple and quick operation, high sensitivity, and the like; and metered by the concentration of hydrogen peroxide, the molar absorption coefficient is up to 3.6*104L.mol-1.cm-1.

The invention discloses a method for measuring nitrites by surface enhanced Raman spectroscopy (SERS). The method comprises the following steps of: 1. preparing an analytic solution of nitrates with known concentrations, and measuring the SER scattering peak intensity value at 1506cm<-1> of the analytic solution to be I; 2. preparing a blank control system excluding nitrates, and measuring the SER scattering peak intensity value of the system to be I0; 3. computing delta I equal to I0 minus I; 4. formulating a working curve of the concentrations of nitrates based on delta I; 5. preparing an analytic solution of tested samples, measuring the SER scattering peak intensity value of the analytic solution to be the I sample, and computing the delta I sample equal to I0 minus I sample; and 6. computing the concentrations of nitrates in the tested samples according to the working curve. Compared with the existing methods, the method has the advantages that the method is simple and convenient to operate, and the reagent is accessible and has high sensitivity and good selectivity.

The invention discloses a method for simply and quickly measuring mercury ions based on gold-size nanoprobe which is dressed by trithiocyanuric acid, which is characterized in that: trithiocyanuric acid is adopted as a recognition element of the mercury ions, and gold-size nanoparticles which are dressed by the trithiocyaunuric acid as a probe to realize the colorimetric determination of content of mercury ions in a water solution. By utilizing the method for measuring the mercury ions, no pollution is caused on the environment, selectivity on the mercury ions is good, and the interference of a majority of metal ions can be avoided; and the required instrument is simple, the price is low, and the method is suitable for being used in simple and crude fields such as countryside and the like.

The invention provides a vertical graphene nanoribbon and preparation thereof, and application of the vertical grapheme nanoribbon in preparation of a supercapacitor, belonging to the technical field of preparation of carbon nanomaterials. According to the invention, a silicon wafer is located at a bottom layer; the vertical graphene nanoribbon is arranged on the silicon wafer; and the vertical graphene nanoribbon is prepared from unfolded single-walled carbon nanotubes and stays perpendicular to the silicon wafer. The single-walled carbon nanotubes are allowed to grown vertically on the silicon wafer, and then the single-walled carbon nanotubes are unfolded. The vertical graphene nanoribbon is applied to the supercapacitor.

The invention discloses a preparation method of a mesoporous SiO2-based Ag catalyst and application thereof in CO oxidation. The method is characterized by comprising the following steps: preparing an Ag / SiO2 catalyst by a one-step method by using lauryl amine as a template agent; dissolving the lauryl amine in absolute ethyl alcohol, vigorously stirring in a water bath kettle at a certain temperature to enable the lauryl amine to be completely dissolved; adding silicon sources, de-ionized water, formaldehyde and silver nitrate according to a certain ratio, sequence and time interval, continuing stirring for a period of time, washing with de-ionized water, leaching, drying, and calcining to obtain the Ag / SiO2 catalyst with a mesoporous structure; and using the Ag / SiO2 catalyst in a CO low-temperature catalytic oxidation reaction. The preparation method can prepare the Ag / SiO2 catalyst with a mesoporous structure and a high specific surface area, and the prepared Ag / SiO2 catalyst has high CO low-temperature oxidation catalysis activity and huge application prospects.

The invention relates to a deep processing method of sea cucumber, concretely a preparation method of sea cucumber active polypeptide under ultrasonic extraction. The preparation method comprises thefollowing steps: A preparing fresh paste of sea cucumber; B extracting sea cucumber polypeptide using ultrasonic wave; C purifying sea cucumber polypeptide using ultra-micro film; D performing concentration at low temperature; E centrifugally spray-drying. The positive effects of the invention are that the sea cucumber polypeptide is extracted using the ultrasonic wave technology to increase the deep processing rate of the sea cucumber, effectively keep the active component in the sea cucumber, thus the collagen of the sea cucumber is not invariant and the helical state of the collagen is keptand the activity is added and the active polypeptide of the sea cucumber is uniformly distributed under purification of ultra-micro film; the extraction process does not need high temperature, thereby preventing decomposing the polypeptide, polysaccharide, saponin molecular structure of the sea cucumber due to high temperature and conquering the defects of the conventional extraction that the polypeptide, polysaccharide molecular structure is broken and the activity is reduced; the instrument is simple, the operation is convenient and the autopadicity of the ultrasonoscope is very high and the preparation method is convenient for industrialization. The sea cucumber active polypeptide can be prepared into capsules or tablets capable of invigorating the kidney and supporting yang, benefiting vital energy and invigorating yin, producing and enriching the blood, moistening the intestines and removing the dryness and depressing tumor diffusion.

The invention discloses an efficient liquid chromatogram-atomic fluorescence spectrum method for measuring mercury forms, and belongs to environment analysis technology. The efficient liquid chromatogram-flame atomization-atomic fluorescence spectrum-based method for measuring the mercury forms of methyl mercury, bivalent inorganic mercury, ethyl mercury and the like comprises that: after multiple mercury forms are separated by efficient liquid chromatogram, the mercury is mixed with acid and hydroboron in turn, the organic mercury is converted into hydrogenated organic mercury, the hydrogenated organic mercury is subjected to gas-liquid separation, mercury atoms are further converted in the argon-hydrogen flame, and the mercury is measured by adopting an atomic fluorescence spectroscope. A device for implementing the method comprises a liquid chromatogram pump, a chromatogram column, a peristaltic pump, a three-way valve, a gas-liquid separator, a flame atomizer, the atomic fluorescence spectroscope and a polytetrafluoroethylene tube. The method does not need to use strong oxidant such as potassium sulfate and the like after column treatment or use degradation equipment of ultraviolet, microwave and the like, has the advantages of simple instruments, convenience for operation and environment friendliness, and is suitable for form analysis of the mercury in environments and biological samples.

The invention discloses a red mud adsorbent for removing organic dye pollutants, and a preparation method of the adsorbent. The preparation method comprises the steps: taking red mud and water, and stirring to form a red mud water solution; adding hydrochloric acid into the red mud water solution to obtain a red mud turbid solution; then taking polystyrene microspheres and an ethanol water solution at the mass ratio of 1:25, and evenly mixing under the magnetic stirring, to form a polystyrene microspheres-ethanol water solution; taking the red mud dirty solution and the polystyrene microspheres-ethanol water solution at the volume ratio of 1:2, and performing magnetic stirring to obtain a mixed solution I; and aging for 0.5-1hour, and performing suction filtration on the aged mixture; filtering, washing, drying and grinding a solid product after the suction filtration is carried out, thus obtaining the red mud adsorbent. The preparation method is simple to operate, low in cost, and capable of solving the red mud pollution problem; the prepared red mud adsorbent is a graded porous material, has a spherical large hole and wormhole-shaped mesoporous structure, the aperture of each large hole is 350nm, the average aperture of each mesopore is 4nm, the specific surface area is 232m<2> / g, and the red mud adsorbent has higher organic dye pollutant adsorption property.

The invention discloses a method for acquisition of multi-bud paris polyphylla seedlings, and relates to the technical field of the seed biology. The method comprises the following steps: collecting mature single-bud paris polyphylla fruits and placing until the scarfskin is corrupted, removing the testa, performing the variable temperature sand storage treatment to seeds of which the testa is removed, taking out after the seed germination, performing the asepsis under the aseptic condition, shifting to a medium of 1 / 2 MS + 0.5 mg / L of gibberellins, cultivating in 22 DEG C under the conditions of constant temperature and darkness, and cultivating under the conditions of 500 lx of light intensity, 12 h illumination / 12 h darkness, and 22 DEG C of the constant temperature, seeding in a seedbed after the germination, backfilling with soil, covering by pine needles, and constructing a sunshade net. The method is capable of combining the undiscovered method for changing the germination characters of the seeds with the traditional method for promoting the germination of the paris polyphylla seeds, and changing the single-bud paris polyphylla seeds to the multi-bud paris polyphylla seedlings through the different treatment conditions. The success rate is up to more than 95%.

The qualitative cell analysis method based on microfluid control chip features the single channel microfluid control chip as platform, the sample pond and the buffering pond on two ends of the channel and microscope coupled in any position to the channel. The analysis process includes: feeding cell liquid into the buffering pond and buffering liquid into the waste liquid pond and regulating the liquid surface height in the buffering pond to make cells inside the visual field still; applied voltage across the buffering pond and the waste liquid pond, recording with CCD on the microscope the directional shift speed of each cell and calculating the electrophoresis mobility of each cell; drawing the cell number- electrophoresis mobility curve of the tested cells; and comparing the curve with standard curves to obtain the qualitative cell analysis result. The method of the present invention has less interference and no need of separation or sorting of cell groups.

The invention belongs to the field of deep processing of garlic, and particularly discloses a method for purifying alliinase by double-water-phase separation. The method comprises the following steps: (1) peeling and precooling garlic squamous bulbs, immersing in a precooled extract, homogenizing, centrifuging, and discarding garlic slag, thereby obtaining the supernate crude enzyme solution; (2) dissolving ammonium sulfate, sodium dihydrogen phosphate or trisodium citrate in a Britton-Robinson buffer solution, oscillating to completely dissolve, adding a PEG (polyethylene glycol) solution, evenly mixing by oscillation, and standing for phase splitting, thereby obtaining a double-water-phase extractant; (3) adding the crude enzyme solution into the double-water-phase extractant, oscillating, centrifuging, and standing for phase splitting; and (4) ultrafiltering the lower phase of the enriched alliinase, and carrying out vacuum freeze drying on the trapped fluid to obtain the alliinase solid. The method is simple to operate, has the advantages of simple apparatuses, time saving, low cost, and higher alliinase purification multiple and enzyme activity recovery rate than the prior art, and can easily implement scale-up production.

The invention belongs to the field of the chemistry and chemical industry, specifically relates to a method for identifying sodium carbonate and sodium hydrogen carbonate solution. The invention provides a method for identifying sodium carbonate and sodium hydrogen carbonate solution, which is based on the prior art and is different from prior art. The method uses Cl- ion modulated HgCl2 solution to identify Na2CO3 and NaHCO3 solution at macroconcentration, Na2CO3 solution can produce red brown deposition in Na2CO3 solution after mixed with modulated HgCl2 solution, and NaHCO3 solution can not produce this phenomenon. The method has the merits of simple instrument and convenient operation, common medicine and short time consumption, obvious phenomenon which is easy to detect, high success rate and good effect.

The present invention discloses a resonance scattering spectrometry for measuring ammonia nitrogen in water rapidly, the measurement steps is that 1. a standard system of ammonium muriate is prepared, and a scattering intensity I is mensurated at a point of 447m; 2. a blank system without ammonium muriate is prepared, and a scattering intensity I0 is mensurated; 3. calculation of Delta I447m = I-I0; 4. a working curve is made according to the concentration relation of the Delta I447m to the ammonium muriate; 5. a detection system is prepared by adding a measuring object containing ammonium muriate with ignorance concentration, and Delta I447M of the measuring system is obtained; 6. according to the working curve, the ammonium muriate concentration of the measuring object is calculated. Comparing with the existing method, the instrument of the method of the invention is simple, operation is easy, reagent is obtained easily, sensitivity is high, measurement range is wide, and cost is low.

The invention discloses a preparation method for synthesizing a photo-catalytic material by using a MOF as a precursor, and belongs to the field of catalysts. The method adopts a manner that the MOF is used as the precursor to synthesize the photo-catalytic material. Firstly, a metal organic framework MOF of bismuth (such as CAU-17) with uniform morphology and stable structure is synthesized, theMOF and a metal salt aqueous solution are stirred under a water bath condition, a mixed solution formed after stirring is subjected to centrifugal drying, and calcination is carried out at a certain temperature to obtain the photo-catalytic material which can maintain a MOF appearance frame. The method has the advantages of short cycle, large output, simple process operation, stable product performance, good catalytic performance, and being not easy to cause secondary pollution. The method is suitable for large-scale mass production, and has a relatively broad scientific research and practicalvalue.

The invention relates to a preparation method of a high internal phase Pickering emulsion with stable egg white protein, which comprises the following steps: stirring egg white at a medium speed, filtering to obtain an egg white protein solution, and carrying out ultrasonic treatment to obtain a modified egg white protein solution; carrying out high-speed shearing on the gel obtained by egg whiteprotein heat treatment, carrying out pre-homogenization, and carrying out ultrasonic crushing by using an ultrasonic probe to form egg white protein microgel particle dispersion liquid; the dispersionliquid and vegetable oil are homogenized under the condition of stirring for 2-4 min at the speed of 12,000 rpm to 15,000 rpm, and stable high internal-phase Pickering emulsion is obtained; the egg white protein microgel particles have good emulsibility, and the prepared high internal-phase emulsion has high safety and good stability and is still stable after being stored at 4 DEG C for more than30 days. The method can be applied to the fields of food, cosmetics and medicines.

The invention provides a chemiluminescence immune detection kit for detecting a pro-gastrin-releasing peptide; the chemiluminescence immune detection kit is composed of a non-transparent white elisa plate coated with the pro-gastrin-releasing peptide, a pro-gastrin-releasing peptide standard, a pro-gastrin-releasing peptide-peroxidase labelled antibody working liquid, a luminous substrate liquid, a concentrated sample diluent and a concentrated detergent. A pro-gastrin-releasing peptide protein conjugate is obtained by coupling the pro-gastrin-releasing peptide with a carrier protein by a mixed anhydride method or a carbodiimide method, and the concentrated detergent contains 0.05% of Twain-20. Compared with a conventional enzyme-linked immunosorbent assay, the kit has higher sensitivity, is short in detection time and low in cost, and can be used for detection of the pro-gastrin-releasing peptide in human blood or serum.

The invention discloses a preparation method of a compound glass nanopore and application of the compound glass nanopore to biomolecular detection. The preparation method comprises the following specific operation steps of 1, preparing a glass nanopore; 2, modifying phospholipids on the glass nanopore prepared in the step 1; 3, preparing the compound glass nanopore; and 4, measuring current data between electrodes with the glass nanopore modified with the phospholipids provided with SWCNT in an inserted manner through a picoammeter and a patch clamp and recording the current data. According tothe preparation method, the glass nanopore is pulled by utilizing a glass puller, and the phospholipids provided with the SWCNT in an inserted manner are modified in the glass nanopore through the capillary phenomenon of nanopores. The operations of ultrasonically cutting the long-time purified SWCNT and utilizing a microinjection probe to insert the SWCNT into bimolecular layers of the phospholipids are not required, so that the method is simple. The current signal changes of different biomacromolecules can be measured through a specific pore of the compound glass nanopore, so that the compound glass nanopore is suitable for detecting the other different target molecules, has universality and is beneficial for application and popularization.